1887

Abstract

Peripheral blood leukocytes (PBLs) and tissue samples from 36 sheep were examined for jaagsiekte sheep retrovirus (JSRV) by hemi-nested PCR. Animals were classified according to the status of sheep pulmonary adenomatosis (SPA), which was confirmed by pathological examination, as follows: (i) sheep with classical SPA (cSPA, =10), (ii) sheep with atypical SPA (aSPA, =6), (iii) non-affected sheep from SPA-affected flocks (in-contact, =10) and (iv) non-affected sheep from SPA-free flocks (control, =10). JSRV proviral DNA was detected in the PBLs of 10/10 cSPA, 5/6 aSPA, 4/10 in-contact and 0/10 control sheep. Lung tumours and lymphoid organs were also found to be JSRV-positive. The number of positive PCR results was greater for sheep in the cSPA group than for those in the aSPA and in-contact groups. For the first time, it is concluded that JSRV can be detected in naturally infected sheep before the onset of clinical disease and even before the development of discernible tumours.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-6-1355
2001-06-01
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/6/0821355a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-6-1355&mimeType=html&fmt=ahah

References

  1. Callahan, R. & Smith, G. H. ( 2000; ). MMTV-induced mammary tumorigenesis: gene discovery, progression to malignancy and cellular pathways. Oncogene 19, 992-1001.[CrossRef]
    [Google Scholar]
  2. Garcı́a-Goti, M. (1999). Estudios sobre la patogenia y diagnóstico de la adenomatosis pulmonar ovina. PhD thesis, University of Zaragoza, Spain. Edited by Servicio Central de Publicaciones del Gobierno Vasco, Vitoria, Spain. D. L. VI 245–2000.
  3. Garcı́a-Goti, M., González, L., Cousens, C., Cortabarrı́a, N., Extramiana, A. B., Minguijón, E., Ortı́n, A., de las Heras, M. & Sharp, J. M. ( 2000; ). Sheep pulmonary adenomatosis: characterization of two pathological forms associated with jaagsiekte retrovirus. Journal of Comparative Pathology 122, 55-65.[CrossRef]
    [Google Scholar]
  4. González, L. (1990). El Maedi o neumonı́a progresiva en el conjunto de las enfermedades respiratorias crónicas del ganado ovino en la Comunidad Autónoma Vasca. PhD thesis, Universidad de Zaragoza, Spain. Edited by Servicio Central de Publicaciones del Gobierno Vasco, Vitoria, Spain. D. L. VI 546–1990.
  5. Holland, M. J., Palmarini, M., Garcı́a-Goti, M., González, L., McKendrick, I., De las Heras, M. & Sharp, J. M. ( 1999; ). Jaagsiekte retrovirus is widely distributed both in T and B lymphocytes and in mononuclear phagocytes of sheep with naturally and experimentally acquired pulmonary adenomatosis. Journal of Virology 73, 4004-4008.
    [Google Scholar]
  6. Ortı́n, A., Minguijón, E., Dewar, P., Garcı́a, M., Palmarini, M., González, L., Sharp, J. M. & De las Heras, M. ( 1998; ). Lack of specific immunoresponse against recombinant capsid antigen of the sheep pulmonary adenomatosis retrovirus in sheep and goats naturally affected by enzootic intranasal tumour or jaagsiekte. Veterinary Immunology and Immunopathology 61, 229-237.[CrossRef]
    [Google Scholar]
  7. Palmarini, M., Dewar, P., De las Heras, M., Inglis, N. F., Dalziel, R. G. & Sharp, J. M. ( 1995; ). Epithelial tumour cells in the lungs of sheep with pulmonary adenomatosis are major sites of replication for Jaagsiekte retrovirus. Journal of General Virology 76, 2731-2737.[CrossRef]
    [Google Scholar]
  8. Palmarini, M., Holland, J. M., Cousens, C., Dalziel, R. G. & Sharp, J. M. ( 1996; ). Jaagsiekte retrovirus establishes a disseminated infection of the lymphoid tissues of sheep affected by pulmonary adenomatosis. Journal of General Virology 77, 2991-2998.[CrossRef]
    [Google Scholar]
  9. Palmarini, M., Sharp, J. M., De las Heras, M. & Fan, H. ( 1999; ). Jaagsiekte sheep retrovirus is necessary and sufficient to induce a contagious lung cancer of sheep. Journal of Virology 73, 6964-6972.
    [Google Scholar]
  10. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  11. Sharp, J. M. & Herring, A. J. ( 1983; ). Sheep pulmonary adenomatosis: demonstration of a protein which cross-reacts with the major core proteins of Mason–Pfizer monkey virus and mouse mammary tumour virus. Journal of General Virology 64, 2323-2327.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-6-1355
Loading
/content/journal/jgv/10.1099/0022-1317-82-6-1355
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error