1887

Abstract

Potyviruses, such as potato virus Y and tobacco etch virus, as well as cucumber mosaic cucumovirus, interfere with post-transcriptional gene silencing (PTGS). When RedStar-type cultivars, whose flowers have alternating white and pigmented sectors, were infected with these viruses, each virus induced a different pattern of restoration of floral anthocyanin pigmentation. Local reversion to coloured phenotypes in the white sectors, which occurred through interference with PTGS of the chalcone synthase A () gene, was correlated with locally increased levels of mRNA and virus concentration. Our results show that virus infection can interfere with PTGS of a native plant gene, and that this can have profound effects on symptom expression.

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2001-05-01
2024-04-19
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References

  1. Boerjan W., Bauw G., van Montagu M., Inzé D. 1994; Distinct phenotypes generated by overexpression and suppression of S-adenosyl-l-methionine synthetase reveal developmental patterns of gene silencing in tobacco. Plant Cell 6:1401–1414
    [Google Scholar]
  2. Carrère I., Tepfer M., Jacquemond M. 1998; Recombinants of cucumber mosaic virus (CMV): determinants of host range and symptomatology. Archives of Virology 144:365–79
    [Google Scholar]
  3. Chrzanowska M. 1991; New isolates of the necrotic strain of potato virus Y (PVYN) found in Poland. Potato Research 34:179–182
    [Google Scholar]
  4. Fire A. 1999; RNA-triggered gene silencing. Trends in Genetics 5:358–363
    [Google Scholar]
  5. Höfgen R., Axelsen K. B., Kannangara C. G., Schütte I., Pohlenz H. D., Willmitzer L., Grimm B., von Wettstein D. 1994; A visible marker for antisense mRNA expression in plants: inhibition of chlorophyll synthesis with a glutamate-1 semialdehyde aminotransferase antisense gene. Proceedings of the National Academy of Sciences, USA 91:1726–1730
    [Google Scholar]
  6. Jorgensen R. A. 1995; Cosuppression, flower color patterns, and metastable gene expression states. Science 268:686–691
    [Google Scholar]
  7. Kinney A. J. 1997; Development of genetically engineered soybean oils for food applications. Journal of Food Lipids 3:273–292
    [Google Scholar]
  8. Kooter J. M., Matzke M. A., Meyer P. 1999; Listening to the silent genes: transgene silencing, gene regulation and pathogen control. Trends in Plant Sciences 4:340–347
    [Google Scholar]
  9. Kunz C., Schöb H., Stam M., Kooter J. M., Meins F. 1996; developmentally regulated silencing and reactivation of tobacco chitinase transgene expression. Plant Journal 10:437–450
    [Google Scholar]
  10. Legnani R., Gognalons P., Gébré-Sélassié K., Marchoux G., Moretti A., Laterrot H. 1996; Identification and characterization of resistance to tobacco etch virus in Lycopersicon species. Plant Disease 80:306–309
    [Google Scholar]
  11. Llave C., Kasschau K. D., Carrington J. C. 2000; Virus-encoded suppressor of posttranscriptional gene silencing targets a maintenance step in the silencing pathway. Proceedings of the National Academy of Sciences, USA 97:13401–13406
    [Google Scholar]
  12. Loebenstein G., Cohen J., Shabtai S., Coutts R. H. A., Wood K. R. 1977; Distribution of cucumber mosaic virus in systemically infected tobacco leaves. Virology 81:117–125
    [Google Scholar]
  13. Mäki-Valkama T., Valkonen J. P. T., Kreuze J. F., Pehu E. 2000; Transgenic resistance to PVYo associated with post-transcriptional silencing of P1 transgene is overcome by PVYn strains that carry highly homologous P1 sequences and recover transgene expression at infection. Molecular Plant–Microbe Interactions 13:366–373
    [Google Scholar]
  14. Metzlaff M., O’Dell M., Cluster P. D., Flavell R. B. 1997; RNA-mediated RNA degradation and chalcone synthase A silencing in petunia. Cell 88:845–854
    [Google Scholar]
  15. Napoli C., Lemieux C., Jorgensen R. 1990; Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans . Plant Cell 2:279–289
    [Google Scholar]
  16. Palauqui J.-C., Elmayan T., Dorlhac de Borne F., Crété P., Charles C., Vaucheret H. 1996; Frequencies, timing, and spatial patterns of co-suppression of nitrate reductase and nitrite reductase in transgenic tobacco plants. Plant Physiology 112:1447–1456
    [Google Scholar]
  17. Palauqui J. C., Elmayan T., Pollien J. M., Vaucheret H. 1997; Systemic acquired silencing: transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions. EMBO Journal 16:4738–4745
    [Google Scholar]
  18. Ratcliff F. G., McFarlane S. A., Baulcombe D. C. 1999; Gene silencing without DNA: RNA-mediated cross protection between viruses. Plant Cell 11:1207–1215
    [Google Scholar]
  19. van Blokland R., van der Geest N., Mol J. N. M., Kooter J. M. 1994; Transgene-mediated suppression of chalcone synthase expression in Petunia hybrida results from an increase in RNA turnover. Plant Journal 6:861–877
    [Google Scholar]
  20. van der Krol A. R., Mur L. A., Beld M., Mol J. N. M., Stuitje A. R. 1990; Flavonoid genes in Petunia : addition of a limited number of gene copies may lead to a suppression of gene expression. Plant Cell 2:291–299
    [Google Scholar]
  21. Voinnet O., Pinto Y., Baulcombe D. C. 1999; Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants. Proceedings of the National Academy of Sciences, USA 96:14147–14152
    [Google Scholar]
  22. Waterhouse P. M., Smith N. A., Wang M. B. 1999; Virus resistance and gene silencing: killing the messenger. Trends in Plant Sciences 4:452–457
    [Google Scholar]
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