1887

Abstract

The molecular basis of pathogenicity of the chicken anaemia virus (CAV) needs to be clarified in order to develop a safe, live virus vaccine. In this study, several high- and low-pathogenic infectious DNA clones were obtained from field virus samples after 12 or 38 passages in MDCC-MSB1 cells. The high-pathogenic clones induced a low haematocrit, low weight gain and high mortality. Nucleotide sequence analyses identified one amino acid, at residue 394 of the VP1 capsid protein, as a major determinant of pathogenicity. To determine the role of this amino acid in pathogenicity, chimeric infectious DNA clones and point-mutated clones were used for chicken pathogenicity tests. These analyses clearly demonstrated that residue 394 of VP1 was crucial for the pathogenicity of CAV; all of the cloned viruses with glutamine at this position were highly pathogenic, whereas those with histidine had low pathogenicity. Low-pathogenic CAV, based on an infectious DNA clone, is a candidate for a genetically homogeneous and stable CAV live vaccine.

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2001-05-01
2024-11-12
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References

  1. Akiyama Y., Kato S. 1974; Two cell lines from lymphomas of Marek’s disease. Biken Journal 17:105–116
    [Google Scholar]
  2. Chettle N. J., Eddy R. K., Wyeth P. J., Lister S. A. 1989; An outbreak of disease due to chicken anaemia agent in broiler chickens in England. Veterinary Record 124:211–215
    [Google Scholar]
  3. Claessens J. A. J., Schrier C. C., Mockett A. P. A., Jagt E. H. J. M., Sondermeijer P. J. A. 1991; Molecular cloning and sequence analysis of the genome of chicken anaemia agent. Journal of General Virology 72:2003–2006
    [Google Scholar]
  4. Danen-Van Oorschot A. A., Fischer D. F., Grimbergen J. M., Klein B., Zhuang S., Falkenburg J. H., Backendorf C., Quax P. H., van der Eb A. J., Noteborn M. H. M. 1997; Apoptin induces apoptosis in human transformed and malignant cells but not in normal cells. Proceedings of the National Academy of Sciences, USA 94:5843–5847
    [Google Scholar]
  5. Goryo M., Sugimura H., Matsumoto S., Umemura T., Itakura C. 1985; Isolation of an agent inducing chicken anaemia. Avian Pathology 14:483–496
    [Google Scholar]
  6. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  7. Hoop R. K. 1992; Persistence and vertical transmission of chicken anaemia agent in experimentally infected laying hens. Avian Pathology 21:493–501
    [Google Scholar]
  8. Imai K., Yuasa N. 1990; Development of a microtest method for serological and virological examinations of chicken anemia agent. Japanese Journal of Veterinary Science 52:873–875
    [Google Scholar]
  9. McNulty M. S. 1991; Chicken anaemia agent: a review. Avian Pathology 20:187–203
    [Google Scholar]
  10. McNulty M. S., McIlroy S. G., Bruce D. W., Todd D. 1991; Economic effects of subclinical chicken anemia agent infection in broiler chickens. Avian Diseases 35:263–268
    [Google Scholar]
  11. Meehan B. M., Todd D., Creelan J. L., Connor T. J., McNulty M. S. 1997; Investigation of the attenuation exhibited by a molecularly cloned chicken anemia virus isolate by utilizing a chimeric virus approach. Journal of Virology 71:8362–8367
    [Google Scholar]
  12. Noteborn M. H. M., Koch G. 1995; Chicken anaemia virus infection: molecular basis of pathogenicity. Avian Pathology 24:11–31
    [Google Scholar]
  13. Noteborn M. H. M., de Boer G. F., van Roozelaar D. J., Karreman C., Kranenburg O., Vos J. G., Jeurissen S. H. M., Hoeben R. C., Zantema A., Koch G., Ormondt H., van der Eb A. J. 1991; Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle. Journal of Virology 65:3131–3139
    [Google Scholar]
  14. Noteborn M. H. M., Todd D., Verschueren C. A. J., de Gauw H. W. F. M., Curran W. L., Veldkamp S., Douglas A. J., McNulty M. S., van der Eb A. J., Koch G. 1994a; A single chicken anemia virus protein induces apoptosis. Journal of Virology 68:346–351
    [Google Scholar]
  15. Noteborn M. H. M., Verschueren C. A. J., Zantema A., Koch G., van der Eb A. J. 1994b; Identification of the promoter region of chicken anemia virus (CAV) containing a novel enhancer-like element. Gene 150:313–318
    [Google Scholar]
  16. Noteborn M. H. M., Verschueren C. A. J., van Ormondt H., van der Eb A. J. 1998; Chicken anemia virus strains with a mutated enhancer/promoter region share reduced virus spread and cytopathogenicity. Gene 223:165–172
    [Google Scholar]
  17. Otaki Y., Nunoya T., Tajima M., Tamada H., Nomura Y. 1987; Isolation of chicken anaemia agent and Marek’s disease virus from chickens vaccinated with turkey herpesvirus and lesions induced in chicks by inoculating both agents. Avian Pathology 16:291–306
    [Google Scholar]
  18. Pietersen A. M., van der Eb M. M., Rademaker H. J., van den Wollenberg D. J. M., Rabelink M. J. W. E., Kuppen P. J. K., van Dierendonck J. H., van Ormondt H., Masman D., Van de Velde C. J. H., van der Eb A. J., Hoeben R. C., Noteborn M. H. M. 1999; Specific tumor-cell killing with adenovirus vectors containing the apoptin gene. Gene Therapy 6:882–892
    [Google Scholar]
  19. Rosenberger J. K., Cloud S. S. 1989; The effects of age, route of exposure, and coinfection with infectious bursal disease virus on the pathogenicity and transmissibility of chicken anemia agent (CAA). Avian Diseases 33:753–759
    [Google Scholar]
  20. Scott A. N. J., Connor T. J., Creelan J. L., McNulty M. S., Todd D. 1999; Antigenicity and pathogenicity characteristics of molecularly cloned chicken anaemia virus isolates obtained after multiple cell culture passages. Archives of Virology 144:1961–1975
    [Google Scholar]
  21. Studdert M. J. 1993; Circoviridae: new viruses of pigs, parrots and chickens. Australian Veterinary Journal 70:121–122
    [Google Scholar]
  22. Takagi S., Goryo M., Okada K. 1996; Pathogenicity for chicks of six isolates of chicken anemia virus (CAV) isolated in the past two years (‘93 ‘94). Journal of the Japanese Society on Poultry Diseases 32:90–97 (in Japanese
    [Google Scholar]
  23. Taniguchi T., Yuasa N., Maeda M., Horiuchi T. 1982; Hematopathological changes in dead and moribund chicks induced by chicken anemia agent. National Institute of Animal Health Quarterly (Tokyo) 22:61–69
    [Google Scholar]
  24. Todd D., Connor T. J., Calvert V. M., Creelan J. L., Meehan B. M., McNulty M. S. 1995; Molecular cloning of an attenuated chicken anaemia virus isolate following repeated cell culture passage. Avian Pathology 24:171–187
    [Google Scholar]
  25. Todd D., Connor T. J., Creelan J. L., Borghmans B. J., Calvert V. M., McNulty M. S. 1998; Effect of multiple cell culture passages on the biological behaviour of chicken anaemia virus. Avian Pathology 27:74–79
    [Google Scholar]
  26. Yamaguchi S., Kaji N., Munang’andu H. M., Kojima C., Mase M., Tsukamoto K. 2000; Quantification of chicken anaemia virus by competitive polymerase chain reaction. Avian Pathology 29:305–310
    [Google Scholar]
  27. Yuasa N., Imai K. 1986; Pathogenicity and antigenicity of eleven isolates of chicken anaemia agent (CAA). Avian Pathology 15:639–645
    [Google Scholar]
  28. Yuasa N., Taniguchi T., Yoshida I. 1979; Isolation and some characteristics of an agent inducing anemia in chicks. Avian Diseases 23:366–385
    [Google Scholar]
  29. Yuasa N., Noguchi T., Furuta K., Yoshida I. 1980; Maternal antibody and its effect on the susceptibility of chicks to chicken anemia agent. Avian Diseases 24:197–202
    [Google Scholar]
  30. Yuasa N., Imai K., Watanabe K., Saito F., Abe M., Komi K. 1987; Aetiological examination of an outbreak of haemorrhagic syndrome in a broiler flock in Japan. Avian Pathology 16:521–526
    [Google Scholar]
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