Demonstration by single-cell PCR that Reed–Sternberg cells and bystander B lymphocytes are infected by different Epstein–Barr virus strains in Hodgkin’s disease Free

Abstract

Epstein–Barr virus (EBV) is associated with Hodgkin’s disease (HD). However, EBV-positive Reed–Sternberg (RS) cells and EBV-positive B lymphocytes co-exist in the same EBV-positive lymph node affected by HD. In a previous report, using total lymph node DNA, the presence of two distinct EBV strains was demonstrated, but their cellular localization (i.e. RS cells vs B lymphocytes) could not be determined. To address this question, three patients with EBV-associated HD were selected in the present study and single-cell PCR of the latent membrane protein-1 () gene from isolated RS cells was performed. In one case, it was clear that RS cells and B lymphocytes were infected by different EBV strains. In the two remaining cases, only one band was detected from total lymph node DNA. However, single-cell PCR showed that RS cells in each sample were infected by single EBV strains, which were different from those detected in lymphoblastoid cell lines derived from EBV-positive B lymphocytes of lymph node cell suspensions from these two patients.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-5-1169
2001-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/5/0821169a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-5-1169&mimeType=html&fmt=ahah

References

  1. Anagnostopoulos I., Herbst H., Niedobitek G., Stein H. 1989; Demonstration of monoclonal EBV genomes in Hodgkin’s disease and Ki-1-positive anaplastic large cell lymphoma by combined Southern blot and in situ hybridization. Blood 74:810–816
    [Google Scholar]
  2. Baer R., Bankier A. T., Biggin M. D., Deininger P. L., Farrell P. J., Gibson T. J., Hatfull G., Hudson G. S., Satchwell S. C., Seguin C. and others 1984; DNA sequence and expression of the B95-8 Epstein–Barr virus genome. Nature 310:207–211
    [Google Scholar]
  3. Baichwal V. R., Sugden B. 1988; Transformation of Balb 3T3 cells by the BNLF-1 gene of Epstein–Barr virus. Oncogene 2:461–467
    [Google Scholar]
  4. Berger C., van Baarle D., Kersten M. J., Klein M. R., Al-Homsi A. S., Dunn B., McQuain C., van Oers R., Knecht H. 1999; Carboxy terminal variants of Epstein–Barr virus-encoded latent membrane protein 1 during long-term human immunodeficiency virus infection: reliable markers for individual strain identification. Journal of Infectious Diseases 179:240–244
    [Google Scholar]
  5. Brousset P., Meggetto F., Chittal S., Bibeau F., Arnaud J., Rubin B., Delsol G. 1993; Assessment of the methods for the detection of Epstein–Barr virus nucleic acids and related gene products in Hodgkin’s disease. Laboratory Investigation 69:483–490
    [Google Scholar]
  6. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. 1984; Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes. Journal of Histochemistry and Cytochemistry 32:219–229
    [Google Scholar]
  7. Delsol G., Brousset P., Chittal S., Rigal-Huguet F. 1992; Correlation of the expression of Epstein–Barr virus latent membrane protein and in situ hybridization with biotinylated Bam HI-W probes in Hodgkin’s disease. American Journal of Pathology 140:247–253
    [Google Scholar]
  8. Gravel S., Delsol G., Al Saati T. 1998; Single-cell analysis of the t(14; 18)(q32; q21) chromosomal translocation in Hodgkin’s disease demonstrates the absence of this translocation in neoplastic Hodgkin and Reed–Sternberg cells. Blood 91:2866–2874
    [Google Scholar]
  9. Herbst H., Niedobitek G., Kneba M., Hummel M., Finn T., Anagnostopoulos I., Bergholz M., Krieger G., Stein H. 1990; High incidence of Epstein–Barr virus genomes in Hodgkin’s disease. American Journal of Pathology 137:13–18
    [Google Scholar]
  10. Herbst H., Dallenbach F., Hummel M., Niedobitek G., Pileri S., Muller-Lantzsch N., Stein H. 1991; Epstein–Barr virus latent membrane protein expression in Hodgkin and Reed–Sternberg cells. Proceedings of the National Academy of Sciences, USA 88:4766–4770
    [Google Scholar]
  11. Hummel M., Anagnostopoulos I., Dallenbach F., Korbjuhn P., Dimmler C., Stein H. 1992; EBV infection patterns in Hodgkin’s disease and normal lymphoid tissue: expression and cellular localization of EBV gene products. British Journal of Haematology 82:689–694
    [Google Scholar]
  12. Izumi K. M., Kieff E. D. 1997; The Epstein–Barr virus oncogene product latent membrane protein 1 engages the tumor necrosis factor receptor-associated death domain protein to mediate B lymphocyte growth transformation and activate NF-κB. Proceedings of the National Academy of Sciences, USA 94:12592–12597
    [Google Scholar]
  13. Kaye K. M., Devergne O., Harada J. N., Izumi K. M., Yalamanchili R., Kieff E., Mosialos G. 1996; Tumor necrosis factor receptor associated factor 2 is a mediator of NF-κB activation by latent infection membrane protein 1, the Epstein–Barr virus transforming protein. Proceedings of the National Academy of Sciences, USA 93:11085–11090
    [Google Scholar]
  14. Knecht H., Bachmann E., Brousset P., Sandvej K., Nadal D., Bachmann F., Odermatt B. F., Delsol G., Pallesen G. 1993; Deletions within the LMP1 oncogene of Epstein–Barr virus are clustered in Hodgkin’s disease and identical to those observed in nasopharyngeal carcinoma. Blood 82:2937–2942
    [Google Scholar]
  15. Kuppers R., Hansmann M. L., Rajewsky K. 1998; Clonality and germinal centre B-cell derivation of Hodgkin/Reed–Sternberg cells in Hodgkin’s disease. Annals of Oncology 9:S17–S20
    [Google Scholar]
  16. Li S. N., Chang Y. S., Liu S. T. 1996; Effect of a 10-amino acid deletion on the oncogenic activity of latent membrane protein 1 of Epstein–Barr virus. Oncogene 12:2129–2135
    [Google Scholar]
  17. Meggetto F., Muller C., Henry S., Selves J., Mariame B., Brousset P., Al Saati T., Delsol G. 1996; Epstein–Barr virus (EBV)-associated lymphoproliferations in severe combined immunodeficient mice transplanted with Hodgkin’s disease lymph nodes: implications of EBV-positive bystander B lymphocytes rather than EBV-infected Reed–Sternberg cells. Blood 87:2435–2442
    [Google Scholar]
  18. Meggetto F., Brousset P., Selves J., Delsol G., Mariame B. 1997; Reed–Sternberg cells and ‘bystander’ lymphocytes in lymph nodes affected by Hodgkin’s disease are infected with different strains of Epstein–Barr virus. Journal of Virology 71:2547–2549
    [Google Scholar]
  19. Mehl A. M., Fischer N., Rowe M., Hartmann F., Daus H., Trumper L., Pfreundschuh M., Muller-Lantzsch N., Grasser F. A. 1998; Isolation and analysis of two strongly transforming isoforms of the Epstein–Barr-virus (EBV)-encoded latent membrane protein-1 (LMP1) from a single Hodgkin’s lymphoma. International Journal of Cancer 76:194–200
    [Google Scholar]
  20. Raab-Traub N. 1989; The human DNA tumor viruses: human papilloma virus and Epstein–Barr virus. Cancer Treatment and Research 47:285–302
    [Google Scholar]
  21. Sato H., Takimoto T., Tanaka S., Tanaka J., Raab-Traub N. 1990; Concatameric replication of Epstein–Barr virus: structure of the termini in virus-producer and newly transformed cell lines. Journal of Virology 64:5295–5300
    [Google Scholar]
  22. Sixbey J. W., Shirley P., Chesney P. J., Buntin D. M., Resnick L. 1989; Detection of a second widespread strain of Epstein–Barr virus. Lancet ii:761–765
    [Google Scholar]
  23. Stein H., Hummel M. 1999; Cellular origin and clonality of classic Hodgkin’s lymphoma: immunophenotypic and molecular studies. Seminars in Hematology 36:233–241
    [Google Scholar]
  24. Wang D., Liebowitz D., Kieff E. 1985; An EBV membrane protein expressed in immortalized lymphocytes transforms established rodent cells. Cell 43:831–840
    [Google Scholar]
  25. Weiss L. M., Strickler J. G., Warnke R. A., Purtilo D. T., Sklar J. 1987; Epstein–Barr viral DNA in tissues of Hodgkin’s disease. American Journal of Pathology 129:86–91
    [Google Scholar]
  26. Weiss L. M., Movahed L. A., Warnke R. A., Sklar J. 1989; Detection of Epstein–Barr viral genomes in Reed–Sternberg cells of Hodgkin’s disease. New England Journal of Medicine 320:502–506
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-5-1169
Loading
/content/journal/jgv/10.1099/0022-1317-82-5-1169
Loading

Data & Media loading...

Most cited Most Cited RSS feed