1887

Journal of General Virology header logo

Volume 82, Issue 4

Towards a protein interaction map of potyviruses: protein interaction matrixes of two potyviruses based on the yeast two-hybrid system Free

Abstract

A map for the interactions of the major proteins from (PVA) and (PSbMV) (members of the genus , family ) was generated using the yeast two-hybrid system (YTHS). Interactions were readily detected with five PVA protein combinations (HC–HC, HC–CI, VPg–VPg, NIa–NIb and CP–CP) and weak but reproducible interactions were detected for seven additional combinations (P1–CI, P3–NIb, NIaPro–NIb, VPg–NIa, VPg–NIaPro, NIaPro–NIa and NIa–NIa). In PSbMV, readily detectable interactions were found in five protein combinations (HC–HC, VPg–VPg, VPg–NIa, NIa–NIa and NIa–NIb) and weaker but reproducible interactions were detected for three additional combinations (P3–NIa, NIa–NIaPro and CP–CP). The self-interactions of HC, VPg, NIa and CP and the interactions of VPg–NIa, NIa–NIaPro and NIa–NIb were, therefore, common for the two potyviruses. The multiple protein interactions revealed in this study shed light on the co-ordinated functions of potyviral proteins involved in virus movement and replication.

  • Received:
  • Accepted:
  • Published Online:
© Microbiology Society
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-4-935
2001-04-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/4/0820935a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-4-935&mimeType=html&fmt=ahah

References

  1. Arbatova J., Lehto K., Pehu E., Pehu T. 1998; Localization of the P1 protein of potato Y potyvirus in association with cytoplasmic inclusion bodies and in the cytoplasm of infected cells. Journal of General Virology 79:2319–2323
     [Google Scholar]
  2. Bartel P., Chien C. T., Sternglanz R., Fields S. 1993; Elimination of false positives that arise in using the two-hybrid system. Biotechniques 14:920–924
     [Google Scholar]
  3. Bartel P. L., Roecklein J. A., SenGupta D., Fields S. 1996; A protein linkage map of Escherichia coli bacteriophage T7. Nature Genetics 12:72–77
     [Google Scholar]
  4. Berger P. H., Wyatt S. D., Shiel P. J., Silbernagel M. J., Druffel K., Mink G. I. 1997; Phylogenetic analysis of the Potyviridae with emphasis on legume-infecting potyviruses. Archives of Virology 142:1979–1999
     [Google Scholar]
  5. Carrington J. C., Jensen P. E., Schaad M. C. 1998; Genetic evidence for an essential role for potyvirus CI protein in cell-to-cell movement. Plant Journal 14:393–400
     [Google Scholar]
  6. Choi I. R., Stenger D. C., French R. 2000; Multiple interactions among proteins encoded by the mite-transmitted wheat streak mosaic tritimovirus. Virology 267:185–198
     [Google Scholar]
  7. Cronin S., Verchot J., Haldeman-Cahill R., Schaad M. C., Carrington J. C. 1995; Long-distance movement factor: a transport function of the potyvirus helper component proteinase. Plant Cell 7:549–559
     [Google Scholar]
  8. Cuconati A., Xiang W., Lahser F., Pfister T., Wimmer E. 1998; A protein linkage map of the P2 nonstructural proteins of poliovirus. Journal of Virology 72:1297–1307
     [Google Scholar]
  9. Daros J. A., Schaad M. C., Carrington J. C. 1999; Functional analysis of the interaction between VPg-proteinase (NIa) and RNA polymerase (NIb) of tobacco etch potyvirus, using conditional and suppressor mutants. Journal of Virology 73:8732–8740
     [Google Scholar]
  10. Estojak J., Brent R., Golemis E. A. 1995; Correlation of two-hybrid affinity data with in vitro measurements. Molecular and Cellular Biology 10:5820–5829
     [Google Scholar]
  11. Fellers J., Wan J., Hong Y., Collins G. B., Hunt A. G. 1998; In vitro interactions between a potyvirus-encoded, genome-linked protein and RNA-dependent RNA polymerase. Journal of General Virology 79:2043–2049
     [Google Scholar]
  12. Fields S., Song O. 1989; A novel genetic system to detect protein–protein interactions. Nature 340:245–246
     [Google Scholar]
  13. Fromont-Racine M., Rain J. C., Legrain P. 1997; Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens. Nature Genetics 16:277–282
     [Google Scholar]
  14. Guo D., Merits A., Saarma M. 1999; Self-association and mapping of interaction domains of helper component-proteinase of potato A potyvirus. Journal of General Virology 80:1127–1131
     [Google Scholar]
  15. Hollenberg S. M., Sternglanz R., Cheng P. F., Weintraub H. 1995; Identification of a new family of tissue-specific basic helix-loop-helix proteins with a two-hybrid system. Molecular and Cellular Biology 15:3813–3822
     [Google Scholar]
  16. Hong Y., Levay K., Murphy J. F., Klein P. G., Shaw J. G., Hunt A. G. 1995; A potyvirus polymerase interacts with the viral coat protein and VPg in yeast cells. Virology 214:159–166
     [Google Scholar]
  17. Ito T., Tashiro K., Muta S., Ozawa R., Chiba T., Nishizawa M., Yamamoto K., Kuhara S., Sakaki Y. 2000; Toward a protein–protein interaction map of the budding yeast: a comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins. Proceedings of the National Academy of Sciences, USA 97:1143–1147
     [Google Scholar]
  18. Kasschau K. D., Cronin S., Carrington J. C. 1997; Genome amplification and long-distance movement functions associated with the central domain of tobacco etch potyvirus helper component-proteinase. Virology 228:251–262
     [Google Scholar]
  19. Li X. H., Valdez P., Olvera R. E., Carrington J. C. 1997; Functions of the tobacco etch virus RNA polymerase (NIb): subcellular transport and protein–protein interaction with VPg/proteinase (NIa). Journal of Virology 71:1598–1607
     [Google Scholar]
  20. Luciano C. S., Murphy J. F., Rhoads R. E., Shaw J. G. 1991; VPg-mediated aggregation of potyviral RNA. Journal of General Virology 72:205–208
     [Google Scholar]
  21. Mendelsohn A. R., Brent R. 1999; Protein interaction methods–toward an endgame. Science 284:1948–1950
     [Google Scholar]
  22. Merits A., Guo D., Saarma M. 1998; VPg, coat protein and five non-structural proteins of potato A potyvirus bind RNA in a sequence-unspecific manner. Journal of General Virology 79:3123–3127
     [Google Scholar]
  23. Merits A., Guo D., Järvekülg L., Saarma M. 1999; Biochemical and genetic evidence for interactions between potato A potyvirus-encoded proteins P1 and P3 and proteins of the putative replication complex. Virology 263:15–22
     [Google Scholar]
  24. O’Reilly E. K., Tang N., Ahlquist P., Kao C. C. 1995; Biochemical and genetic analyses of the interaction between the helicase-like and polymerase-like proteins of the brome mosaic virus. Virology 214:59–71
     [Google Scholar]
  25. Oruetxebarria I., Guo D., Merits A., Mäkinen K., Saarma M., Valkonen J. P. T. 2001; Identification of the genome-linked protein of a Potato virus A , with comparison to other members of genus Potyvirus. Virus Research 73:103–112
     [Google Scholar]
  26. Phizicky E. M., Fields S. 1995; Protein–protein interactions: methods for detection and analysis. Microbiological Reviews 59:94–123
     [Google Scholar]
  27. Revers F., Le Gall O., Candresse T., Maule A. J. 1999; New advances in understanding the molecular biology of plant/potyvirus interactions. Molecular Plant–Microbe Interactions 12:367–376
     [Google Scholar]
  28. Riechmann J. L., Lain S., Garcia J. A. 1992; Highlights and prospects of potyvirus molecular biology. Journal of General Virology 73:1–16
     [Google Scholar]
  29. Rojas M. R., Zerbini F. M., Allison R. F., Gilbertson R. L., Lucas W. J. 1997; Capsid protein and helper component-proteinase function as potyvirus cell-to-cell movement proteins. Virology 237:283–295
     [Google Scholar]
  30. Schiestl R. H., Gietz R. D. 1989; High efficiency transformation of intact yeast cells using single-stranded nucleic acids as a carrier. Current Genetics 16:339–346
     [Google Scholar]
  31. Uetz P., Giot L., Cagney G., Mansfield T. A., Judson R. S., Knight J. R., Lockshon D., Narayan V., Srinivasan M., Pochart P., Qureshi-Emili A., Li Y., Godwin B., Conover D., Kalbfleisch T., Vijayadamodar G., Yang M., Johnston M., Fields S., Rothberg J. M. 2000; A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae. Nature 403:623–627
     [Google Scholar]
  32. Urcuqui-Inchima S., Walter J., Drugeon G., German-Retana S., Haenni A. L., Candresse T., Bernardi F., Le Gall O. 1999; Potyvirus helper component-proteinase self-interaction in the yeast two-hybrid system and delineation of the interaction domain involved. Virology 258:95–99
     [Google Scholar]
  33. Vidal M., Legrain P. 1999; Yeast forward and reverse ‘n’-hybrid systems. Nucleic Acids Research 27:919–929
     [Google Scholar]
  34. Xiang W., Cuconati A., Hope D., Kirkegaard K., Wimmer E. 1998; Complete protein linkage map of poliovirus P3 proteins: interaction of polymerase 3Dpol with VPg and with genetic variants of 3AB. Journal of Virology 72:6732–6741
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-4-935
Loading
Towards a protein interaction map of potyviruses: protein interaction matrixes of two potyviruses based on the yeast two-hybrid system
J. Gen. Virol. 82, 935 (2001); https://doi.org/10.1099/0022-1317-82-4-935
/content/journal/jgv/10.1099/0022-1317-82-4-935
/content/journal/jgv/10.1099/0022-1317-82-4-935
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error