1887

Abstract

The population structure and genetic variation of (CYSDV) isolates were estimated by single-strand conformation polymorphism and nucleotide sequence analyses of the CYSDV coat protein gene. Analysis of 71 isolates collected from Spain, Jordan, Turkey, Lebanon, Saudi Arabia and North America showed that, from a genetic viewpoint, these isolates could be divided into two diverged subpopulations: an Eastern subpopulation composed of Saudi Arabian isolates and a Western subpopulation containing the rest of the CYSDV isolates. The genetic variation within the Western subpopulation was very small (nucleotide identity >99%) in spite of the extensive and discontinuous geographical distribution and different years of collection. We also estimated the within-isolate genetic structure and variation of three CYSDV isolates by analysing 30 clones per isolate. Our results showed that these CYSDV isolates had a quasispecies structure.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-4-929
2001-04-01
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/4/0820929a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-4-929&mimeType=html&fmt=ahah

References

  1. Abou-Jawdah, Y., Sobh, H., Fayad, A., Lecoq, H., Del colle, B. & Trad-Ferr , J. ( 2000; ). Cucurbit yellow stunting disorder virus – a new threat to cucurbits in Lebanon. Journal of Plant Pathology 82, 55–60.
    [Google Scholar]
  2. Alicai, T., Fenby, N. S., Gibson, R. W., Adipala, E., Vetten, H. J., Foster, G. D. & Seal, S. E. ( 1999; ). Occurrence of two serotypes of sweet potato chlorotic stunt virus in East Africa and their associated differences in coat protein and HSP70 homologue gene sequences. Plant Pathology 48, 718-726.[CrossRef]
    [Google Scholar]
  3. Berdiales, B., Bernal, J. J., Sáez, E., Woudt, B. & Rodrı́guez-Cerezo, E. ( 1999; ). Occurrence of cucurbit yellow stunting disorder virus (CYSDV) and beet pseudo-yellows virus in cucurbit crops in Spain and transmission of CYSDV by two biotypes of Bemisia tabaci. European Journal of Plant Pathology 105, 211-215.[CrossRef]
    [Google Scholar]
  4. Brown, J. K., Frohlich, D. R. & Rosell, R. C. ( 1995; ). The sweet potato or silverleaf whiteflies: biotypes of Bemisia tabaci or a species complex? Annual Review of Entomology 40, 511-534.[CrossRef]
    [Google Scholar]
  5. Célix, A., López-Sesé, A., Almarza, N., Gómez-Guillamón, M. L. & Rodrı́guez-Cerezo, E. ( 1996; ). Characterization of cucurbit yellow stunting disorder virus, a Bemisia tabaci-transmitted closterovirus. Phytopathology 86, 1370-1376.
    [Google Scholar]
  6. Domingo, E. & Holland, J. J. ( 1997; ). RNA virus mutations and fitness for survival. Annual Review of Microbiology 51, 151-178.[CrossRef]
    [Google Scholar]
  7. Enomoto, N., Kurosaki, M., Tanaka, Y., Marumo, F. & Sato, C. ( 1994; ). Fluctuation of hepatitis C virus quasispecies in persistent infection and interferon treatment revealed by single-strand conformation polymorphism analysis. Journal of General Virology 75, 1361-1369.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1989; ). PHYLIP: Phylogenetic Inference Package (version 3.2). Cladistics 5, 164-166.
    [Google Scholar]
  9. Fraile, A., Malpica, J. M., Aranda, M. A., Rodrı́guez-Cerezo, E. & Garcı́a-Arenal, F. ( 1996; ). Genetic diversity in tobacco mild green mosaic tobamovirus infecting the wild plant Nicotiana glauca. Virology 223, 148-155.[CrossRef]
    [Google Scholar]
  10. Hassan, A. A. & Duffus, J. E. ( 1991; ). A review of a yellowing and stunting disorder in the United Arab Emirates. Emirates Journal of Agricultural Sciences 2, 1-16.
    [Google Scholar]
  11. Holland, J. & Domingo, E. ( 1998; ). Origin and evolution of viruses. Virus Genes 16, 13-21.[CrossRef]
    [Google Scholar]
  12. Holland, J. J., Spindler, K., Horodyski, F., Grabau, E., Nichol, S. & VandePol, S. ( 1982; ). Rapid evolution of RNA genomes. Science 215, 1577-1582.[CrossRef]
    [Google Scholar]
  13. Kao, J., Jia, L., Tian, T., Rubio, L. & Falk, B. W. ( 2000; ). First report of Cucurbit yellow stunting disorder virus (Genus Crinivirus) in North America. Plant Disease 84, 101.
    [Google Scholar]
  14. Kong, P., Rubio, L., Polek, M. & Falk, B. W. ( 2000; ). Population structure and genetic diversity of California citrus tristeza virus (CTV) field isolates. Virus Genes 21, 139-145.[CrossRef]
    [Google Scholar]
  15. Li, W.-H. ( 1993; ). Unbiased estimation of the rates of synonymous and nonsynonymous substitutions. Journal of Molecular Evolution 36, 96-99.[CrossRef]
    [Google Scholar]
  16. Livieratos, I. C., Avgelis, A. D. & Coutts, R. H. A. ( 1999; ). Molecular characterization of the cucurbit yellow stunting disorder virus coat protein gene. Phytopathology 89, 1050-1055.[CrossRef]
    [Google Scholar]
  17. Lynch, M. & Crease, T. J. ( 1990; ). The analysis of population survey data on DNA sequence variation. Molecular Biology and Evolution 7, 377-394.
    [Google Scholar]
  18. Martelli, G. P., Agranovsky, A. A., Bar-Joseph, M., Boscia, D., Candresse, T., Coutts, R. H. A., Dolja, V. V., Duffus, J. E., Falk, B. W., Gonsalves, D., Jelkmann, W., Karasev, A. V., Minafra, A., Murant, A., Namba, S., Niblett, C. L., Vetten, H. J. & Yoshikawa, N. ( 2000; ). Family Closteroviridae. In Seventh Report of the International Committee on Taxonomy of Viruses , pp. 943-952. Edited by M. H. V. Van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. New York & San Diego: Academic Press.
  19. Moya, A., Rodrı́guez-Cerezo, E. & Garcı́a-Arenal, F. ( 1993; ). Genetic structure of natural populations of the plant RNA virus tobacco mild green mosaic virus. Molecular Biology and Evolution 10, 449-456.
    [Google Scholar]
  20. Orita, M., Suzuki, Y., Sekiya, T. & Hayashi, K. ( 1989; ). Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5, 874-879.[CrossRef]
    [Google Scholar]
  21. Pamilo, P. & Bianchi, N. O. ( 1993; ). Evolution of the Zfx and Zfy genes: rates and interdependence between the genes. Molecular Biology and Evolution 10, 271-281.
    [Google Scholar]
  22. Rodrı́guez-Cerezo, E., Moya, A. & Garcı́a-Arenal, F. ( 1989; ). Variability and evolution of the plant RNA virus pepper mild mottle virus. Journal of Virology 63, 2198-2203.
    [Google Scholar]
  23. Roossinck, M. J. ( 1997; ). Mechanisms of plant virus evolution. Annual Review of Phytopathology 35, 1953-1965.
    [Google Scholar]
  24. Rubio, L., Ayllón, M. A., Guerri, J., Pappu, H., Niblett, C. & Moreno, P. ( 1996; ). Differentiation of citrus tristeza closterovirus (CTV) isolates by single-strand conformation polymorphism analysis of the coat protein gene. Annals of Applied Biology 129, 479-489.[CrossRef]
    [Google Scholar]
  25. Rubio, L., Soong, J., Kao, J. & Falk, B. W. ( 1999; ). Geographic distribution and molecular variation of isolates of three whitefly-borne closteroviruses of cucurbits: lettuce infectious yellows virus, cucurbit yellow stunting disorder virus, and beet pseudo-yellows virus. Phytopathology 89, 707-711.[CrossRef]
    [Google Scholar]
  26. Rubio, L., Guerri, J. & Moreno, P. (2000). Characterization of citrus tristeza virus isolates by single strand conformation polymorphism analysis of DNA complementary to their RNA population. In Proceedings of the 14th Conference of the International Organization of Citrus Virologists. Edited by R. H. Yokomi, J. V. da Graca & R. F. Lee. Riverside, California: IOCV (in press).
  27. Schneider, W. L. & Roossinck, M. J. ( 2000; ). Evolutionary related Sindbis-like plant viruses maintain different levels of population diversity in a common host. Journal of Virology 74, 3130-3134.[CrossRef]
    [Google Scholar]
  28. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673-4680.[CrossRef]
    [Google Scholar]
  29. Wisler, G. C., Duffus, J. E., Liu, H. Y. & Li, R. H. ( 1998; ). Ecology and epidemiology of whitefly-transmitted Closteroviruses. Plant Disease 82, 270-280.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-4-929
Loading
/content/journal/jgv/10.1099/0022-1317-82-4-929
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error