1887

Journal of General Virology header logo

Volume 82, Issue 4

A novel variant genotype C of hepatitis B virus identified in isolates from Australian Aborigines: complete genome sequence and phylogenetic relatedness Free

Abstract

There have been no reports of DNA sequences of hepatitis B virus (HBV) strains from Australian Aborigines, although the hepatitis B surface antigen (HBsAg) was discovered among them. To investigate the characteristics of DNA sequences of HBV strains from Australian Aborigines, the complete nucleotide sequences of HBV strains were determined and subjected to molecular evolutionary analysis. Serum samples positive for HBsAg were collected from five Australian Aborigines. Phylogenetic analysis of the five complete nucleotide sequences compared with DNA sequences of 54 global HBV isolates from international databases revealed that three of the five were classified into genotype D and were most closely related in terms of evolutionary distance to a strain isolated from a healthy blood donor in Papua New Guinea. Two of the five were classified into a novel variant genotype C, which has not been reported previously, and were closely related to a strain isolated from Polynesians, particularly in the X and Core genes. These two strains of variant genotype C differed from known genotype C strains by 5·9–7·4% over the complete nucleotide sequence and 4·0–5·6% in the small-S gene, and had residues Arg, Thr and Lys, characteristic of serotype , which have not been reported previously in genotype C. In conclusion, this is the first report of the characteristics of complete nucleotide sequences of HBV from Australian Aborigines. These results contribute to the investigation of the worldwide spread of HBV, the relationship between serotype and genotype and the ancient common origin of Australian Aborigines.

  • Received:
  • Accepted:
  • Published Online:
© Microbiology Society
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-4-883
2001-04-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/4/0820883a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-4-883&mimeType=html&fmt=ahah

References

  1. Alexopoulou A., Karayiannis P., Hadziyannis S. J., Hou J., Pickering J., Luo K., Thomas H. C. 1996; Whole genome analysis of hepatitis B virus from four cases with fulminant hepatitis: genetic variability and its potential role in disease pathogenicity. Journal of Viral Hepatitis 3:173–181
     [Google Scholar]
  2. Asahina Y., Enomoto N., Ogura Y., Sakuma I., Kurosaki M., Izumi N., Marumo F., Sato C. 1996; Complete nucleotide sequences of hepatitis B virus genomes associated with epidemic fulminant hepatitis. Journal of Medical Virology 48:171–178
     [Google Scholar]
  3. Blumberg B. S., Alter H. J., Visnich S. 1965; A ‘new’ new antigen in leukemia sera. Journal of the American Medical Association 191:541–546
     [Google Scholar]
  4. Campbell D. H., Sargent J. W., Plant A. J. 1989; The prevalence of markers of infection with hepatitis B virus in a mixed-race Australian community. Medical Journal of Australia 150:489–492
     [Google Scholar]
  5. Couroucé A. M., Holland P. V., Muller J. Y., Soulier J. P. 1976; HBs antigen subtypes. Bibliotheca Haematologica 42:1
     [Google Scholar]
  6. Couroucé-Pauty A.-M., Lemaire J. M., Roux J. F. 1978; New hepatitis B surface antigen subtypes inside the ad category. Vox Sanguinis 35:304–308
     [Google Scholar]
  7. Galibert F., Mandart E., Fitoussi F., Tiollais P., Charnay P. 1979; Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature 281:646–650
     [Google Scholar]
  8. Hasegawa K., Huang J., Rogers S. A., Blum H. E., Liang T. J. 1994; Enhanced replication of a hepatitis B virus mutant associated with an epidemic of fulminant hepatitis. Journal of Virology 68:1651–1659
     [Google Scholar]
  9. Holman C. D. J., Bucen M. R., Quadros C. F., Reid P. M. 1987; Occurrence and distribution of hepatitis B infection in the aboriginal population of Western Australia. Australia and New Zealand Journal of Medicine 17:518–525
     [Google Scholar]
  10. Horikita M., Itoh S., Yamamoto K., Shibayama T., Tsuda F., Okamoto H. 1994; Differences in the entire nucleotide sequence between hepatitis B virus genomes from carriers positive for antibody to hepatitis B e antigen with and without active disease. Journal of Medical Virology 44:96–103
     [Google Scholar]
  11. Ina Y. 1994; ODEN: a program package for molecular evolutionary analysis and database search of DNA and amino acid sequences. Computer Applications in the Biosciences 10:11–12
     [Google Scholar]
  12. Kim K. T., Hyun S. W., Kim Y. S., Rho H. M. 1988; Complete nucleotide sequence of hepatitis B virus (subtype adr). Korean Journal of Biochemistry 21:319–331
     [Google Scholar]
  13. Lee W. M. 1997; Hepatitis B virus infection. New England Journal of Medicine 337:1733–1745
     [Google Scholar]
  14. Magnius L. O., Norder H. 1995; Subtypes, genotypes and molecular epidemiology of the hepatitis B virus as reflected by sequence variability of the S-gene. Intervirology 38:24–34
     [Google Scholar]
  15. Monkongdee P., Boonchird C., Balachandra K., Thawaranantha D., Watanaseree J., Pantuwatana S. 1998; Cloning and sequence analysis of hepatitis B virus genome of adr subtype isolated in Thailand. Journal of the Science Society of Thailand 24:155–167
     [Google Scholar]
  16. Norder H., Hammas B., Löfdahl S., Couroucé A.-M., Magnius L. O. 1992; Comparison of the amino acid sequences of nine different serotypes of hepatitis B surface antigen and genomic classification of the corresponding hepatitis B virus strains. Journal of General Virology 73:1201–1208
     [Google Scholar]
  17. Norder H., Hammas B., Lee S.-D., Bile K., Couroucé A.-M., Mushahwar I. K., Magnius L. O. 1993; Genetic relatedness of hepatitis B viral strains of diverse geographical origin and natural variations in the primary structure of the surface antigen. Journal of General Virology 74:1341–1348
     [Google Scholar]
  18. Norder H., Couroucé A.-M., Magnius L. O. 1994; Complete genomes, phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology 198:489–503
     [Google Scholar]
  19. Okamoto H., Imai M., Shimozaki M., Hoshi Y., Iizuka H., Gotanda T., Tsuda F., Miyakawa Y., Mayumi M. 1986; Nucleotide sequence of a cloned hepatitis B virus genome, subtype ayr : comparison with genomes of the other three subtypes. Journal of General Virology 67:2305–2314
     [Google Scholar]
  20. Okamoto H., Tsuda F., Sakugawa H., Sastrosoewignjo R. I., Imai M., Miyakawa Y., Mayumi M. 1988; Typing hepatitis B virus by homology in nucleotide sequence: comparison of surface antigen subtypes. Journal of General Virology 69:2575–2583
     [Google Scholar]
  21. Patterson F., Bumak J., Batey R. 1993; Changing prevalence of hepatitis B virus in urbanized Australian aborigines. Journal of Gastroenterology and Hepatology 8:410–413
     [Google Scholar]
  22. Roberts R. G., Jones R., Smith M. A. 1990; Thermoluminescence dating of a 50,000-year-old human occupation site in northern Australia. Nature 345:153–156
     [Google Scholar]
  23. Roberts-Thomson J. M., Martinson J. J., Norwich J. T., Harding R. M., Clegg J. B., Boettcher B. 1996; An ancient common origin of aboriginal Australians and New Guinea highlanders is supported by α-globin haplotype analysis. American Journal of Human Genetics 58:1017–1024
     [Google Scholar]
  24. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406–425
     [Google Scholar]
  25. Stoll-Becker S., Repp R., Glebe D., Schaefer S., Kreuder J., Kann M., Lampert F., Gerlich W. H. 1997; Transcription of hepatitis B virus in peripheral blood mononuclear cells from persistently infected patients. Journal of Virology 71:5399–5407
     [Google Scholar]
  26. Stuyver L., De Gendt S., Van Geyt C., Zoulim F., Fried M., Schinazi R. F., Rossau R. 2000; A new genotype of hepatitis B virus: complete genome and phylogenetic relatedness. Journal of General Virology 81:67–74
     [Google Scholar]
  27. Takahashi K., Akahane Y., Hino K., Ohta Y., Mishiro S. 1998; Hepatitis B virus genomic sequence in the circulation of hepatocellular carcinoma patients: comparative analysis of 40 full-length isolates. Archives of Virology 143:2313–2326
     [Google Scholar]
  28. Tong S. P., Li J. S., Vitvitski L., Trepo C. 1990; Active hepatitis B virus replication in the presence of anti-HBe is associated with viral variants containing an inactive pre-C region. Virology 176:596–603
     [Google Scholar]
  29. White P. J., O’Connell J. F. 1982 A Prehistory of Australia, New Guinea and Sahul Sydney: Academic Press;
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-4-883
Loading
A novel variant genotype C of hepatitis B virus identified in isolates from Australian Aborigines: complete genome sequence and phylogenetic relatedness
J. Gen. Virol. 82, 883 (2001); https://doi.org/10.1099/0022-1317-82-4-883
/content/journal/jgv/10.1099/0022-1317-82-4-883
/content/journal/jgv/10.1099/0022-1317-82-4-883
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error