1887

Abstract

Human T-cell leukaemia/lymphotropic virus type 1 (HTLV-1), aetiologically linked to lymphoproliferative as well as inflammatory diseases, infects and activates CD4 helper T-cells and thus alters immunoregulatory pathways. The viral regulatory Tax protein has been shown previously to induce the expression of vascular cell adhesion molecule-1 (VCAM-1) by T-cells. To determine the functional role of this adhesion molecule, Jurkat T-cells stably expressing either Tax or both Tax and Rex (another viral regulatory protein) were used in binding and coculture assays performed with either control Jurkat cells or primary human T-lymphocytes. Evidence was provided that VCAM-1 acting in synergy with leucocyte function-associated antigen-3 promotes T-cell–T-cell interactions and increases T-cell proliferation. Interestingly, Rex was found to modulate these events. These data establish that VCAM-1 induced by Tax on T-cells thus contributes to the immunopathological process triggered by HTLV-1 infection.

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2001-04-01
2019-10-17
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References

  1. Al-Fahim, A., Cabre, P., Kastrukoff, L., Dorovini-Zis, K. & Oger, J. ( 1999; ). Blood mononuclear cells in patients with HTLV-I-associated myelopathy: lymphocytes are highly activated and adhesion to endothelial cells is increased. Cellular Immunology 198, 1-10.[CrossRef]
    [Google Scholar]
  2. Daenke, S., McCracken, S. A. & Booth, S. ( 1999; ). Human T-cell leukaemia/lymphoma virus type 1 syncytium formation is regulated in a cell-specific manner by ICAM-1, ICAM-3 and VCAM-1 and can be inhibited by antibodies to integrin β2 or β7. Journal of General Virology 80, 1429-1436.
    [Google Scholar]
  3. Dhawan, S., Weeks, B. S., Abbasi, F., Gralnick, H. R., Notkins, A. L., Klotman, M. E., Yamada, K. M. & Klotman, P. E. ( 1993; ). Increase expression of alpha4-beta1 and alpha5-beta1 integrins on HTLV-I-infected lymphocytes. Virology 197, 778-781.[CrossRef]
    [Google Scholar]
  4. Hildreth, J. E., Subramanium, A. & Hampton, R. A. ( 1997; ). Human T-cell lymphotropic virus type 1 (HTLV-1)-induced syncytium formation mediated by vascular cell adhesion molecule-1: evidence for involvement of cell adhesion molecules in HTLV-1 biology. Journal of Virology 71, 1173-1180.
    [Google Scholar]
  5. Höllsberg, P. ( 1999; ). Mechanisms of T-cell activation by human T-cell lymphotropic virus type I. Microbiology and Molecular Biology Reviews 63, 308-333.
    [Google Scholar]
  6. Huet, S., Wakasugi, H., Sterkers, G., Gilmour, J., Tursz, T., Boumsell, L. & Bernard, A. ( 1986; ). T cell activation via CD2 (T, gp50): the role of accessory cells in activating resting T cells via CD2. Journal of Immunology 137, 1420-1428.
    [Google Scholar]
  7. Ichinose, K., Nakamura, T., Kawakami, A., Eguchi, K., Nagasato, K., Shibayama, K., Tsujihata, M. & Nagataki, S. ( 1992; ). Increased adherence of T cells to human endothelial cells in patients with human T-cell lymphotropic virus type I-associated myelopathy. Archives of Neurology 49, 74-76.[CrossRef]
    [Google Scholar]
  8. Ichinose, K., Nakamura, T., Nishiura, Y., Nagasato, K., Ohishi, K., Watanabe, H., Fujita, A., Kurouji, K.-i., Tsujihata, M. & Nagataki, S. ( 1994; ). Characterization of adherent T cells to human endothelial cells in patients with HTLV-I-associated myelopathy. Journal of the Neurological Sciences 122, 204-209.[CrossRef]
    [Google Scholar]
  9. Ishikawa, T., Imura, A., Tanaka, K., Shirane, H., Okuma, M. & Uchiyama, T. ( 1993; ). E-selectin and vascular cell adhesion molecule-1 mediate adult T-cell leukemia cell adhesion to endothelial cells. Blood 82, 1590-1598.
    [Google Scholar]
  10. Kambara, C., Nakamura, T., Furuya, T., Migita, K., Ida, H., Kawakami, A., Shirabe, S., Nakane, S., Kinoshita, I. & Eguchi, K. ( 1999; ). Vascular cell adhesion molecule-1-mediated matrix metalloproteinase-2 induction in peripheral blood T cells is up-regulated in patients with HTLV-I-associated myelopathy. Journal of Neuroimmunology 99, 242-247.[CrossRef]
    [Google Scholar]
  11. Kanamori, H., Suzuki, N., Siomi, H., Nosaka, T., Sato, A., Sabe, H., Hatanaka, M. & Honjo, T. ( 1990; ). HTLV-1 p27rex stabilizes human interleukin-2 receptor alpha chain mRNA. EMBO Journal 9, 4161-4166.
    [Google Scholar]
  12. Lemasson, I., Robert-Hebmann, V., Hamaia, S., Duc Dodon, M., Gazzolo, L. & Devaux, C. ( 1997; ). Transrepression of lck gene expression by human T-cell leukemia virus type 1-encoded p40tax. Journal of Virology 71, 1975-1983.
    [Google Scholar]
  13. McGuire, K. L., Curtiss, V. E., Larson, E. L. & Haseltine, W. A. ( 1993; ). Influence of human T-cell leukemia virus type 1 tax and rex on interleukin-2 gene expression. Journal of Virology 67, 1590-1599.
    [Google Scholar]
  14. Tanaka, Y., Fukudome, K., Hayashi, M., Takagi, S. & Yoshie, O. ( 1995; ). Induction of ICAM-1 and LFA-3 by Tax1 of human T-cell leukemia virus type 1 and mechanism of down-regulation of ICAM-1 or LFA-1 in adult-T-cell-leukemia cell lines. International Journal of Cancer 60, 554-561.[CrossRef]
    [Google Scholar]
  15. Uchiyama, T. ( 1997; ). Human T cell leukemia virus type I (HTLV-I) and human diseases. Annual Review of Immunology 15, 15-37.[CrossRef]
    [Google Scholar]
  16. Uchiyama, T., Ishikawa, T. & Imura, A. ( 1996; ). Cell adhesion molecules in HTLV-I infection. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology 13 (Suppl. 1), S114–S118.[CrossRef]
    [Google Scholar]
  17. Valentin, H., Lemasson, I., Hamaia, S., Casse, H., König, S., Devaux, C. & Gazzolo, L. ( 1997; ). Transcriptional activation of the vascular cell adhesion molecule-1 gene in T lymphocytes expressing human T-cell leukemia virus type 1 Tax protein. Journal of Virology 71, 8522-8530.
    [Google Scholar]
  18. Wake, A., Tanaka, Y., Nakatsuka, K., Misago, M., Oda, S., Morimoto, I. & Eto, S. ( 1995; ). Calcium-dependent homotypic adhesion through leukocyte function-associated antigen-1/intracellular adhesion molecule-1 induces interleukin-1 and parathyroid hormone-related protein production on adult T-cell leukemia cells in vitro. Blood 86, 2257-2267.
    [Google Scholar]
  19. Weil, R., Levraud, J. P., Dodon, M. D., Bessia, C., Hazan, U., Kourilsky, P. & Israel, A. ( 1999; ). Altered expression of tyrosine kinases of the Src and Syk families in human T-cell leukemia virus type 1-infected T-cell lines. Journal of Virology 73, 3709-3717.
    [Google Scholar]
  20. Weston, S. A. & Parish, C. R. ( 1990; ). New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. Journal of Immunological Methods 133, 87-97.[CrossRef]
    [Google Scholar]
  21. Wucherpfennig, K. W., Höllsberg, P., Richardson, J. H., Benjamin, D. & Hafler, D. A. ( 1992; ). T-cell activation by autologous human T-cell leukemia virus type-I-infected T-cell clones. Proceedings of the National Academy of Sciences, USA 89, 2110-2114.[CrossRef]
    [Google Scholar]
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