1887

Abstract

Infectious pancreatic necrosis virus (IPNV), a member of the with two double-stranded RNA genome segments, encodes five proteins designated VP1 to VP5. To study the function of the 17 kDa nonstructural protein VP5 during virus replication several mutated IPNV genome segments A were constructed and included in a reverse genetics system for IPNV to obtain recombinant virus. Mutations between nt 68 and 85 or nt 94 and 103 in the noncoding region failed to yield viable virus. Only mutations located between nt 86 and 92 and downstream of nt 104 were tolerated, and viable virus could be generated. All IPNV generated showed no difference in replication compared with the wild-type IPNV, indicating that the absence of expression of VP5 did not influence virus growth . Furthermore, the results presented here indicate that initiation of translation of VP5 occurs at position 113, the second in-frame start codon.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-4-805
2001-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/4/0820805a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-4-805&mimeType=html&fmt=ahah

References

  1. Birghan C., Mundt E., Gorbalenya A. E. 2000; A non-canonical Lon proteinase deficient of the ATPase domain employs the Ser–Lys catalytic dyad to impose broad control over the life cycle of a double-stranded RNA virus. EMBO Journal 19:114–123
    [Google Scholar]
  2. Boot H. J., ter Huurne A. A., Peeters B. P., Gielkens A. L. 1999; Efficient rescue of infectious bursal disease virus from cloned cDNA: evidence for involvement of the 3′-terminal sequence in genome replication. Virology 265:330–341
    [Google Scholar]
  3. Cashdollar L. W., Esparza J., Hudson G. R., Chmelo R., Lee P. W. K., Joklik W. K. 1982; Cloning of double-stranded RNA genes of reovirus: sequence of the cloned S2 gene. Proceedings of the National Academy of Sciences, USA 79:7644–7648
    [Google Scholar]
  4. Castric J. 1997; Viral diseases in fish mariculture. Bulletin of the European Association of Fish Pathologists 17:197–200
    [Google Scholar]
  5. Conzelmann K.-K., Meyers G. 1996; Genetic engineering of animal RNA viruses. Trends in Microbiology 4:386–393
    [Google Scholar]
  6. Dobos P. 1995; The molecular biology of infectious pancreatic necrosis virus (IPNV). Annual Review of Fish Diseases 5:24–54
    [Google Scholar]
  7. Duncan R., Dobos P. 1986; The nucleotide sequence of infectious pancreatic necrosis virus (IPNV) dsRNA segment A reveals one large ORF encoding a precursor polyprotein. Nucleic Acids Research 14:5934
    [Google Scholar]
  8. Duncan R., Nagy E., Krell P. J., Dobos P. 1987; Synthesis of the infectious pancreatic necrosis virus polyprotein, detection of a virus encoded protease, and fine structure mapping of genome segment A coding regions. Journal of Virology 61:3655–3664
    [Google Scholar]
  9. Duncan R., Mason R. L., Nagy E., Leong J. A., Dobos P. 1991; Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: a putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motif. Virology 181:541–552
    [Google Scholar]
  10. Frohman M. A., Dush M. K., Martin G. R. 1988; Rapid production of full-length cDNA from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proceedings of the National Academy of Sciences, USA 85:8998–9002
    [Google Scholar]
  11. Heppell J., Tarrab E., Berthiaume L., Lecomte J., Arella M. 1995; Characterization of the small open reading frame on genome segment A of infectious pancreatic necrosis virus. Journal of General Virology 76:2091–2096
    [Google Scholar]
  12. Hill B. J., Way K. 1995; Serological classification of infectious pancreatic necrosis (IPN) virus: a comparison with other birnaviruses. Annual Review of Fish Diseases 5:55–77
    [Google Scholar]
  13. Jarp J., Gjevre A. G., Olsen A. B., Bruheim T. 1994; Risk factors for furunculosis, infectious pancreatic necrosis and mortality in post-smolt of Atlantic salmon, Salmo salar L. Journal of Fish Diseases 18:67–78
    [Google Scholar]
  14. Kordyban S., Magyar G., Chung H. K., Dobos P. 1997; Incomplete dsRNA genomes in purified infectious pancreatic necrosis virus. Virology 239:62–70
    [Google Scholar]
  15. Kozak M. 1986; Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:283–292
    [Google Scholar]
  16. Kozak M. 1995; Adherence to the first-AUG rule when a second AUG codon follows closely upon the first. Proceedings of the National Academy of Sciences, USA 92:2662–2666
    [Google Scholar]
  17. Kozak M. 1997; Recognition of AUG and alternative initiator codons is augmented by G in position +4 but is not generally affected by the nucleotides in positions +5 and +6. EMBO Journal 16:2482–2492
    [Google Scholar]
  18. Kunkel T. A., Roberts J. D., Zakour R. A. 1987; Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods in Enzymology 154:367–382
    [Google Scholar]
  19. Leong J. C., Brown D., Dobos P., Kibenge F. S. B., Ludert J. E., Müller H., Mundt E., Nicholson B. 2000; Family Birnaviridae. Virus Taxonomy: Classification and Nomenclature of Viruses. Seventh Report of the International Committee on Taxonomy of Viruses pp 481–490 Edited by van Regenmortel M. H. V., Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., McGeoch D. J., Maniloff J., Mayo M. A., Pringle C. R., Wickner R. B. San Diego: Academic Press;
    [Google Scholar]
  20. Magyar G., Dobos P. 1994; Evidence for the detection of the infectious pancreatic necrosis virus polyprotein and the 17-kDa polypeptide in infected cells and of the NS protease in purified virus. Virology 204:580–589
    [Google Scholar]
  21. Mandl C. W., Holzmann H., Meixner T., Rauscher S., Stadler P. F., Allison S. L., Heinz F. X. 1998; Spontaneous and engineered deletions in the 3′ noncoding region of tick-borne encephalitis virus: construction of highly attenuated mutants of a flavivirus. Journal of Virology 72:2132–2140
    [Google Scholar]
  22. Müller H., Lange H., Becht H. 1986; Formation, characterization and interfering capacity of a small plaque mutant and of incomplete virus particles of infectious bursal disease virus. Virus Research 4:297–309
    [Google Scholar]
  23. Mundt E., Müller H. 1995; Complete nucleotide sequences of 5′- and 3′-noncoding regions of both genome segments of different strains of infectious bursal disease virus. Virology 209:209–218
    [Google Scholar]
  24. Mundt E., Vakharia V. N. 1996; Synthetic transcripts of double-stranded birnavirus genome are infectious. Proceedings of the National Academy of Sciences, USA 93:11131–11136
    [Google Scholar]
  25. Mundt E., Beyer J., Müller H. 1995; Identification of a novel viral protein in infectious bursal disease virus infected cells. Journal of General Virology 76:437–443
    [Google Scholar]
  26. Mundt E., Köllner B., Kretzschmar D. 1997; VP5 of infectious bursal disease virus is not essential for viral replication in cell culture. Journal of Virology 71:5647–5651
    [Google Scholar]
  27. Petit S., Lejal N., Huet J. C., Delmas B. 2000; Active residues and viral substrate cleavage sites of the protease of the birnavirus infectious pancreatic necrosis virus. Journal of Virology 74:2057–2066
    [Google Scholar]
  28. Rodger H. D., Frerichs G. N. 1997; Clinical infectious pancreatic necrosis virus infection in farmed halibut in the United Kingdom. Veterinary Record 140:401–402
    [Google Scholar]
  29. Wallner G., Mandl C. W., Kunz C., Heinz F. X. 1995; The flavivirus 3′-noncoding region: extensive size heterogeneity independent of evolutionary relationships among strains of tick-borne encephalitis virus. Virology 213:169–178
    [Google Scholar]
  30. Wolf K. 1988 Fish Viruses and Fish Viral Diseases Ithaca, NY: Canstock Publishing Associates/Cornell University Press;
  31. Yao K., Vakharia V. N. 1998; Generation of infectious pancreatic necrosis virus from cloned cDNA. Journal of Virology 72:8913–8920
    [Google Scholar]
  32. Yao K., Goodwin M. A., Vakharia V. N. 1998; Generation of a mutant infectious bursal disease virus that does not cause bursal lesions. Journal of Virology 72:2647–2657
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-4-805
Loading
/content/journal/jgv/10.1099/0022-1317-82-4-805
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error