RT Journal Article SR Electronic(1) A1 Karger, Axel A1 Schmidt, Ulrike A1 Buchholz, Ursula J.YR 2001 T1 Recombinant bovine respiratory syncytial virus with deletions of the G or SH genes: G and F proteins bind heparin JF Journal of General Virology, VO 82 IS 3 SP 631 OP 640 DO https://doi.org/10.1099/0022-1317-82-3-631 PB Microbiology Society, SN 1465-2099, AB Bovine respiratory syncytial virus (BRSV) encodes three transmembrane envelope glycoproteins, namely the small hydrophobic (SH) protein, the attachment glycoprotein (G) and the fusion glycoprotein (F). The BRSV reverse genetics system has been used to generate viable recombinant BRSV lacking either the G gene or the SH gene or both genes. The deletion mutants were fully competent for multicycle growth in cell culture, proving that, of the BRSV glycoprotein genes, the SH and G genes are non-essential. Virus morphogenesis was not impaired by either of the deletions. The deletion mutants were used to study the role of the F glycoprotein and the contributions of SH and G with respect to virus attachment. Attachment mediated by the F protein alone could be blocked by soluble heparin, but not by chondroitin sulphate. Heparin affinity chromatography revealed that both the BRSV G and F glycoproteins have heparin-binding activity, with the affinity of the F glycoprotein being significantly lower than that of G. Therefore, the roles of the BRSV glycoproteins in virus attachment and receptor binding have to be reconsidered., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-82-3-631