1887

Abstract

From October 1997 to January 1998, highly pathogenic H5N2 avian influenza viruses caused eight outbreaks of avian influenza in northern Italy. A nonpathogenic H5N9 influenza virus was also isolated during the outbreaks as a result of virological and epidemiological surveillance to control the spread of avian influenza to neighbouring regions. Antigenic analysis showed that the Italian H5N2 isolates were antigenically similar to, although distinguishable from, A/HK/156/97, a human influenza H5N1 virus isolated in Hong Kong in 1997. Phylogenetic analysis of the haemagglutinin (HA) genes showed that the highly pathogenic Italian viruses clustered with the Hong Kong strains, whereas the nonpathogenic H5N9 virus, despite its epidemiological association with the highly pathogenic Italian isolates, was most closely related to the highly pathogenic A/Turkey/England/91 (H5N1) strain. Like the HA phylogenetic tree, the nonstructural (NS) phylogenetic tree showed that the H5N2 Italian virus genes are clearly separate from those of the H5N9 strain. In contrast, results of the phylogenetic analysis of nucleoprotein (NP) genes indicated a closer genetic relationship between the two Italian virus groups, a finding suggesting a common progenitor. Comparison of the HA, NS and NP genes of the Italian H5 strains with those of the H5N1 viruses simultaneously circulating in Hong Kong revealed that the two groups of viruses do not share a recent common ancestor. No virological and serological evidence of bird-to-human transmission of the Italian H5N2 influenza viruses was found.

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2001-03-01
2019-10-13
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References

  1. Alexander, D. J. ( 2000; ). A review of avian influenza in different bird species. Veterinary Microbiology 74, 3-13.[CrossRef]
    [Google Scholar]
  2. Bean, W. J., Kawaoka, Y., Wood, J. M., Pearson, J. E. & Webster, R. G. ( 1985; ). Characterization of virulent and avirulent A/chicken/Pennsylvania/83 influenza A viruses: potential role of defective interfering RNAs in nature. Journal of Virology 54, 151-160.
    [Google Scholar]
  3. Campitelli, L., Donatelli, I., Foni, E., Castrucci, M. R., Fabiani, C., Kawaoka, Y., Krauss, S. & Webster, R. G. ( 1997; ). Continued evolution of H1N1 and H3N2 influenza viruses in pigs in Italy. Virology 232, 310-318.[CrossRef]
    [Google Scholar]
  4. Capua, I., Marangon, S., Selli, L., Alexander, D. J., Swayne, D. E., Dalla Pozza, M., Parenti, E. & Cancellotti, F. M. ( 1999; ). Outbreaks of highly pathogenic avian influenza (H5N2) in Italy during October 1997 to January 1998. Avian Pathology 28, 455-460.[CrossRef]
    [Google Scholar]
  5. CEC (1992). Council directive 92/40/EEC of 19th May 1992 introducing community measures for the control of avian influenza. Official Journal of the European Communities L167, 1–15.
  6. de Jong, J. C., Claas, E. C., Osterhaus, A. D., Webster, R. G. & Lim, W. L. ( 1997; ). A pandemic warning? Nature 389, 554.
    [Google Scholar]
  7. Garcı́a, M., Crawford, J. M., Latimer, J. W., Rivera-Cruz, E.& Perdue, M. L. ( 1996; ). Heterogeneity in the haemagglutinin gene and emergence of the highly pathogenic phenotype among recent H5N2 avian influenza viruses from Mexico. Journal of General Virology 77, 1493-1504.[CrossRef]
    [Google Scholar]
  8. Guan, Y., Shortridge, K. F., Krauss, S. & Webster, R. G. ( 1999; ). Molecular characterization of H9N2 influenza viruses: were they the donors of the ‘internal’ genes of H5N1 viruses in Hong Kong? Proceedings of the National Academy of Sciences, USA 96, 9363-9367.[CrossRef]
    [Google Scholar]
  9. Guo, Y. J., Krauss, S., Senne, D. A., Mo, I. P., Lo, K. S., Xiong, X. P., Norwood, M., Shortridge, K. F., Webster, R. G. & Guan, Y. ( 2000; ). Characterization of the pathogenicity of members of the newly established H9N2 influenza virus lineages in Asia. Virology 267, 279-288.[CrossRef]
    [Google Scholar]
  10. Horimoto, T., Rivera, E., Pearson, J., Senne, D., Krauss, S., Kawaoka, Y. & Webster, R. G. ( 1995; ). Origin and molecular changes associated with emergence of a highly pathogenic H5N2 influenza virus in Mexico. Virology 213, 223-230.[CrossRef]
    [Google Scholar]
  11. Kawaoka, Y., Nestorowicz, A., Alexander, D. J. & Webster, R. G. ( 1987; ). Molecular analyses of the hemagglutinin genes of H5 influenza viruses: origin of a virulent turkey strain. Virology 158, 218-227.[CrossRef]
    [Google Scholar]
  12. Lu, B.-L., Webster, R. G. & Hinshaw, V. S. ( 1982; ). Failure to detect hemagglutination-inhibiting antibodies with intact avian influenza virions. Infection and Immunity 38, 530-535.
    [Google Scholar]
  13. Mancini, G., Donatelli, I., ArangioRuiz, G. & Rozera, C. ( 1984; ). Detection of antibodies to strain-specific and cross-reactive antigenic determinants on the haemagglutinin of influenza viruses A/Texas/1/77 and A/Bangkok/1/79 in human sera. Brief Report. Archives of Virology 79, 117-121.[CrossRef]
    [Google Scholar]
  14. Matrosovich, M., Zhou, N., Kawaoka, Y. & Webster, R. G. ( 1999; ). The surface glycoproteins of H5 influenza viruses isolated from humans, chickens, and wild aquatic birds have distinguishable properties. Journal of Virology 73, 1146-1155.
    [Google Scholar]
  15. Murphy, T. M. (1986). In Acute Virus Infections of Poultry: A Seminar in the CEC Agricultural Research Programme, p. 23. Edited by J. B. McFerran & M. S. McNulty. Dordrecht: Martinus Nijhoff.
  16. Perdue, M. L., Garcı́a, M., Senne, D. & Fraire, M. ( 1997; ). Virulence-associated sequence duplication at the hemagglutinin cleavage site of avian influenza viruses. Virus Research 49, 173-186.[CrossRef]
    [Google Scholar]
  17. Röhm, C., Horimoto, T., Kawaoka, Y., Süss, J. & Webster, R. G. ( 1995; ). Do hemagglutinin genes of highly pathogenic avian influenza viruses constitute unique phylogenetic lineages? Virology 209, 664-670.[CrossRef]
    [Google Scholar]
  18. Rowe, T., Abernathy, R. A., Hu-Primmer, J., Thompson, W. W., Lu, X., Lim, W., Fukuda, K., Cox, N. J. & Katz, J. M. ( 1999; ). Detection of antibody to avian influenza A (H5N1) virus in human serum by using a combination of serologic assays. Journal of Clinical Microbiology 37, 937-943.
    [Google Scholar]
  19. Senne, D. A., Panigrahy, B., Kawaoka, Y., Pearson, J. E., Süss, J., Lipkind, M., Kida, H. & Webster, R. G. ( 1996; ). Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Diseases 40, 425-437.[CrossRef]
    [Google Scholar]
  20. Shortridge, K. F., Zhou, N. N., Guan, Y., Gao, P., Ito, T., Kawaoka, Y., Kodihalli, S., Krauss, S., Markwell, D., Murti, K. G., Norwood, M., Senne, D., Sims, L., Takada, A. & Webster, R. G. ( 1998; ). Characterization of avian H5N1 influenza viruses from poultry in Hong Kong. Virology 252, 331-342.[CrossRef]
    [Google Scholar]
  21. Suarez, D. L., Perdue, M. L., Cox, N., Rowe, T., Bender, C., Huang, J. & Swayne, D. E. ( 1998; ). Comparisons of highly virulent H5N1 influenza A viruses isolated from humans and chickens from Hong Kong. Journal of Virology 72, 6678-6688.
    [Google Scholar]
  22. Subbarao, K., Klimov, A., Katz, J., Regnery, H., Lim, W., Hall, H., Perdue, M., Swayne, D., Bender, C., Huang, J., Hemphill, M., Rowe, T., Shaw, M., Xu, X., Fukuda, K. & Cox, N. ( 1998; ). Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness. Science 279, 393-396.[CrossRef]
    [Google Scholar]
  23. US Department of Health & Human Services (1982). Concepts and procedures for laboratory-based influenza surveillance. Washington, DC: DHHS, Public Health Service, Centers for Disease Control.
  24. Webster, R. G., Bean, W. J., Gorman, O. T., Chambers, T. M. & Kawaoka, Y. ( 1992; ). Evolution and ecology of influenza A viruses. Microbiological Reviews 56, 152-179.
    [Google Scholar]
  25. Wood, G. W., McCauley, J. W., Bashiruddin, J. B. & Alexander, D. J. ( 1993; ). Deduced amino acid sequences at the haemagglutinin cleavage site of avian influenza A viruses of the H5 and H7 subtypes. Archives of Virology 130, 209-217.[CrossRef]
    [Google Scholar]
  26. Xu, X., Subbarao, K., Cox, N. K. & Guo, Y. ( 1999; ). Genetic characterization of the pathogenic influenza A/Goose/Guangdong/1/96 (H5N1) virus: similarity of its hemagglutinin gene to those of H5N1 viruses from the 1997 outbreaks in Hong Kong. Virology 261, 15-19.[CrossRef]
    [Google Scholar]
  27. Yuen, K. Y., Chan, P. K., Peiris, M., Tsang, D. N., Que, T. L., Shortridge, K. F., Cheung, P. T., To, W. K., Ho, E. T., Sung, R., Cheng, A. F. & Members of the H5N1 Study Group ( 1998; ). Clinical features and rapid viral diagnosis of human disease associated with avian influenza A H5N1 virus. Lancet 351, 467–471.[CrossRef]
    [Google Scholar]
  28. Zhou, N. N., He, S., Zhang, T., Zou, W., Shu, L., Sharp, G. B. & Webster, R. G. ( 1996; ). Influenza infection in humans and pigs in southeastern China. Archives of Virology 141, 649-661.[CrossRef]
    [Google Scholar]
  29. Zhou, N. N., Shortridge, K. F., Claas, E. C. J., Krauss, S. L. & Webster, R. G. ( 1999; ). Rapid evolution of H5N1 influenza viruses in chickens in Hong Kong. Journal of Virology 73, 3366-3374.
    [Google Scholar]
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