1887

Journal of General Virology header logo

Volume 82, Issue 3

Adaptation of primate cell-adapted hepatitis A virus strain HM175 to growth in guinea pig cells is independent of mutations in the 5′ nontranslated region Free

Abstract

Previous studies of hepatitis A virus (HAV) genotypes after adaptation of wild-type virus to growth in cell cultures of primate origin identified determinants for growth in cell culture in the viral 2B and 2C protein-coding regions of the genome and demonstrated that an increased growth efficiency in a particular cell line was achieved by subsequent mutations in the 5′ nontranslated region (5′NTR). The results reported in this study demonstrate that the passage of HAV adapted to primate BS-C-1 cells in guinea pig cells resulted in increased growth efficiency in the rodent cells and decreased growth efficiency in BS-C-1 cells. This adaptation occurred without mutation in the 5′NTR, but the viral 2B and 2C proteins seem to play a role during adaptation to the new environment, as one mutation occurred in each protein. Although the data presented here do not clearly identify which region of the viral genome underwent mutations to improve the interaction of the viruses with guinea pig proteins, they do confirm that the 5′NTR is not the only region responsible for providing host cell-specific information.

  • Received:
  • Accepted:
  • Published Online:
© Microbiology Society
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-3-597
2001-03-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/3/0820597a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-3-597&mimeType=html&fmt=ahah

References

  1. Brack K., Frings W., Dotzauer A., Vallbracht A. 1998; A cytopathogenic, apoptosis-inducing variant of hepatitis A virus. Journal of Virology 72:3370–3376
     [Google Scholar]
  2. Brown E. A., Day S. P., Jansen R. W., Lemon S. M. 1991; The 5′ nontranslated region of hepatitis A virus RNA: secondary structure and elements required for translation in vitro . Journal of Virology 65:5828–5838
     [Google Scholar]
  3. Chang K. H., Brown E. A., Lemon S. M. 1993; Cell type-specific proteins which interact with the 5′ nontranslated region of hepatitis A virus RNA. Journal of Virology 67:6716–6725
     [Google Scholar]
  4. Cromeans T., Sobsey M. D., Fields H. A. 1987; Development of a plaque assay for a cytopathic, rapidly replicating isolate of hepatitis A virus. Journal of Medical Virology 22:45–56
     [Google Scholar]
  5. Day S. P., Murphy P., Brown E. A., Lemon S. M. 1992; Mutations within the 5′ nontranslated region of hepatitis A virus RNA which enhance replication in BS-C-1 cells. Journal of Virology 66:6533–6540
     [Google Scholar]
  6. Dotzauer A., Feinstone S. M., Kaplan G. 1994; Susceptibility of nonprimate cell lines to hepatitis A virus infection. Journal of Virology 68:6064–6068
     [Google Scholar]
  7. Emerson S. U., McRill C., Rosenblum B., Feinstone S. M., Purcell R. H. 1991; Mutations responsible for adaptation of hepatitis A virus to efficient growth in cell culture. Journal of Virology 65:4882–4886
     [Google Scholar]
  8. Emerson S. U., Huang Y. K., McRill C., Lewis M., Purcell R. H. 1992; Mutations in both the 2B and 2C genes of hepatitis A virus are involved in adaptation to growth in cell culture. Journal of Virology 66:650–654
     [Google Scholar]
  9. Funkhouser A. W., Purcell R. H., D’Hondt E., Emerson S. U. 1994; Attenuated hepatitis A virus: genetic determinants of adaptation to growth in MRC-5 cells. Journal of Virology 68:148–157
     [Google Scholar]
  10. Funkhouser A. W., Raychaudhuri G., Purcell R. H., Govindarajan S., Elkins R., Emerson S. U. 1996; Progress toward the development of a genetically engineered attenuated hepatitis A virus vaccine. Journal of Virology 70:7948–7957
     [Google Scholar]
  11. Graff J., Normann A., Feinstone S. M., Flehmig B. 1994; Nucleotide sequence of wild-type hepatitis A virus GBM in comparison to two cell culture-adapted variants. Journal of Virology 68:548–554
     [Google Scholar]
  12. Graff J., Normann A., Flehmig B. 1997; Influence of the 5′ noncoding region of hepatitis A virus strain GBM on its growth in different cell lines. Journal of General Virology 78:1841–1849
     [Google Scholar]
  13. Graff J., Cha J., Blyn L. B., Ehrenfeld E. 1998; Interaction of poly(rC)-binding protein 2 with the 5′ noncoding region of hepatitis A virus RNA and its effects on translation. Journal of Virology 72:9668–9675
     [Google Scholar]
  14. Hornei B., Kämmerer R., Moubayed P., Frings W., Gauss-Müller V., Dotzauer A. 2001; Experimental hepatitis A virus infection in guinea pigs. Journal of Medical Virology (in press)
    [Google Scholar]
  15. Jansen R. W., Newbold J. E., Lemon S. M. 1988; Complete nucleotide sequence of a cell culture-adapted variant of hepatitis A virus: comparison with wild-type virus with restricted capacity for in vitro replication. Virology 163:299–307
     [Google Scholar]
  16. Jecht M., Probst C., Gauss-Müller V. 1998; Membrane permeability induced by hepatitis A virus proteins 2B and 2BC and proteolytic processing of HAV 2BC. Virology 252:218–227
     [Google Scholar]
  17. Lemon S. M., Binn L. N., Marchwicki R. H. 1983; Radioimmunofocus assay for quantitation of hepatitis A virus in cell cultures. Journal of Clinical Microbiology 17:834–839
     [Google Scholar]
  18. Morace G., Pisani G., Beneduce F., Divizia M., Panà A. 1993; Mutations in the 3A genomic region of two cytopathic strains of hepatitis A virus isolated in Italy. Virus Research 28:187–194
     [Google Scholar]
  19. Paul A. V., Tada H., von der Helm K., Wissel T., Kiehn R., Wimmer E., Deinhardt F. 1987; The entire nucleotide sequence of the genome of hepatitis A virus (isolate MBB). Virus Research 8:153–171
     [Google Scholar]
  20. Pestova T. V., Hellen C. U. T., Shatsky I. N. 1996; Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry. Molecular and Cellular Biology 16:6859–6869
     [Google Scholar]
  21. Porter A. G. 1993; Picornavirus nonstructural proteins: emerging roles in virus replication and inhibition of host cell functions. Journal of Virology 67:6917–6921
     [Google Scholar]
  22. Ross B. C., Anderson B. N., Edwards P. C., Gust I. D. 1989; Nucleotide sequence of high-passaged hepatitis A virus strain HM175: comparison with wild-type and cell culture-adapted strains. Journal of General Virology 70:2805–2810
     [Google Scholar]
  23. Schultz D. E., Hardin C. C., Lemon S. M. 1996; Specific interaction of glyceraldehyde 3-phosphate dehydrogenase with the 5′-nontranslated RNA of hepatitis A virus. Journal of Biological Chemistry 271:14134–14142
     [Google Scholar]
  24. Siegl G., Lemon S. M. 1990; Recent advancement in hepatitis A vaccine development. Virus Research 17:75–92
     [Google Scholar]
  25. Tedeschi V., Purcell R. H., Emerson S. U. 1993; Partial characterisation of hepatitis A viruses from three intermediate passage levels of a series resulting in adaptation to growth in cell culture and attenuation of virulence. Journal of Medical Virology 39:16–22
     [Google Scholar]
  26. Yi M., Schultz D. E., Lemon S. M. 2000; Functional significance of the interaction of hepatitis A virus RNA with glyceraldehyde 3-phosphate dehydrogenase (GAPDH): opposing effects of GAPDH and polypyrimidine tract binding protein on internal ribosome entry site function. Journal of Virology 74:6459–6468
     [Google Scholar]
  27. Zhang H., Chao S.-F., Ping L.-H., Grace K., Clarke B., Lemon S. M. 1995; An infectious cDNA clone of a cytopathic hepatitis A virus: genomic regions associated with rapid replication and cytopathic effect. Virology 212:686–697
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-3-597
Loading
Adaptation of primate cell-adapted hepatitis A virus strain HM175 to growth in guinea pig cells is independent of mutations in the 5′ nontranslated region
J. Gen. Virol. 82, 597 (2001); https://doi.org/10.1099/0022-1317-82-3-597
/content/journal/jgv/10.1099/0022-1317-82-3-597
/content/journal/jgv/10.1099/0022-1317-82-3-597
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error