1887

Abstract

Human endogenous retrovirus (HERV) sequences represent about 0·5% of the human genome. The only HERV known to express virus particles is human teratocarcinoma-derived virus (HTDV), which is now termed HTDV/HERV-K. Between 25 and 50 different copies of HERV-K are present in the human genome, three of which contain full-length genes for viral structural proteins. To determine whether genes of different HERV-K proviruses can be expressed, the morphologies and protein expression patterns of HTDV/HERV-K produced by various human teratocarcinoma cell lines were compared. Three different types of retrovirus-like particles were observed, showing differences in the presence of viral surface proteins and the existence of free mature virions. These distinct morphological features between virion types were in accordance with the results of immunoblotting analyses that revealed differences in the cleavage of a viral Gag protein precursor and the presence of a putative Env protein. These data suggest that different HERV-K proviruses are transcribed in human teratocarcinoma cell lines.

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2001-03-01
2019-12-09
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References

  1. Andrews, P. W., Bronson, D. L., Benham, F., Strickland, S. & Knowles, B. B. ( 1980; ). A comparative study of eight cell lines derived from human testicular teratocarcinoma. International Journal of Cancer 26, 269-280.[CrossRef]
    [Google Scholar]
  2. Berkhout, B., Jebbink, M. & Zsiros, J. ( 1999; ). Identification of an active reverse transcriptase enzyme encoded by a human endogenous HERV-K retrovirus. Journal of Virology 73, 2365-2375.
    [Google Scholar]
  3. Boller, K., Frank, H., Löwer, J., Löwer, R. & Kurth, R. ( 1983; ). Structural organization of unique retrovirus-like particles budding from human teratocarcinoma cell lines. Journal of General Virology 64, 2549-2559.[CrossRef]
    [Google Scholar]
  4. Boller, K., König, H., Sauter, M., Mueller-Lantzsch, N., Löwer, R., Löwer, J. & Kurth, R. ( 1993; ). Evidence that HERV-K is the endogenous retrovirus sequence that codes for the human teratocarcinoma-derived retrovirus HTDV. Virology 196, 349-353.[CrossRef]
    [Google Scholar]
  5. Boller, K., Janssen, O., Schuldes, H., Tönjes, R. R. & Kurth, R. ( 1997; ). Characterization of the antibody response specific for the human endogenous retrovirus HTDV/HERV-K. Journal of Virology 71, 4581-4588.
    [Google Scholar]
  6. Bronson, D. L., Fraley, E. E., Fogh, J. & Kalter, S. S. ( 1979; ). Induction of retrovirus particles in human testicular tumor (Tera-1) cell cultures: an electron microscopic study. Journal of the National Cancer Institute 63, 337-339.
    [Google Scholar]
  7. Bronson, D. L., Saxinger, W. C., Ritzi, D. M. & Fraley, E. E. ( 1984; ). Production of virions with retrovirus morphology by human embryonal carcinoma cells in vitro. Journal of General Virology 65, 1043-1051.[CrossRef]
    [Google Scholar]
  8. Conrad, B., Weissmahr, R. N., Boni, J., Arcari, R., Schupbach, J. & Mach, B. ( 1997; ). A human endogenous retroviral superantigen as candidate autoimmune gene in type I diabetes. AIDS Research and Human Retroviruses 90, 303-313.
    [Google Scholar]
  9. Fogh, J. & Trempe, G. (1975). In Human Tumor Cells In Vitro, pp. 115–138. Edited by J. Fogh. New York: Plenum Press.
  10. Frank, H., Schwarz, H., Graf, T. & Schafer, W. ( 1978; ). Properties of mouse leukemia viruses. XV. Electron microscopic studies on the organization of Friend leukemia virus and other mammalian C-type viruses. Zeitschrift für Naturforschung Section C Biosciences 33, 124-138.
    [Google Scholar]
  11. Harris, J. R. ( 1998; ). Placental endogenous retrovirus (ERV): structural, functional, and evolutionary significance. Bioessays 20, 307-316.[CrossRef]
    [Google Scholar]
  12. Kalter, S. S., Helmke, R. J., Heberling, R. L., Panigel, M., Fowler, A. K., Strickland, J. E. & Hellman, A. ( 1973; ). Brief communication: C-type particles in normal human placentas. Journal of the National Cancer Institute 50, 1081-1084.
    [Google Scholar]
  13. Kurth, R., Löwer, R., Löwer, J., Harzmann, R., Pfeiffer, R., Schmidt, C. G., Fogh, J. & Frank, H. (1980). In Cold Spring Harbor Conferences On Cell Proliferation, pp. 835–846. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  14. Li, M. D., Bronson, D. L., Lemke, T. D. & Faras, A. J. ( 1995; ). Restricted expression of new HERV-K members in human teratocarcinoma cells. Virology 208, 733-741.[CrossRef]
    [Google Scholar]
  15. Löwer, R. ( 1999; ). The pathogenic potential of endogenous retroviruses: facts and fantasies. Trends in Microbiology 7, 350-356.[CrossRef]
    [Google Scholar]
  16. Löwer, R., Löwer, J., Frank, H., Harzmann, R. & Kurth, R. ( 1984; ). Human teratocarcinomas cultured in vitro produce unique retrovirus-like viruses. Journal of General Virology 65, 887-898.[CrossRef]
    [Google Scholar]
  17. Löwer, R., Boller, K., Hasenmaier, B., Korbmacher, C., Mueller-Lantzsch, N., Löwer, J. & Kurth, R. ( 1993; ). Identification of human endogenous retroviruses with complex mRNA expression and particle formation. Proceedings of the National Academy of Sciences, USA 90, 4480-4484.[CrossRef]
    [Google Scholar]
  18. Löwer, R., Löwer, J. & Kurth, R. ( 1996; ). The viruses in all of us: characteristics and biological significance of human endogenous retrovirus sequences. Proceedings of the National Academy of Sciences, USA 93, 5177-5184.[CrossRef]
    [Google Scholar]
  19. Löwer, R., Tönjes, R. R., Boller, K., Denner, J., Kaiser, B., Phelps, R. C., Löwer, J., Kurth, R., Badenhoop, K., Donner, H., Usadel, K. H., Miethke, T., Lapatschek, M. & Wagner, H. ( 1998; ). Development of insulin-dependent diabetes mellitus does not depend on specific expression of the human endogenous retrovirus HERV-K. AIDS Research and Human Retroviruses 95, 11-14.
    [Google Scholar]
  20. Medstrand, P., Lindeskog, M. & Blomberg, J. ( 1992; ). Expression of human endogenous retroviral sequences in peripheral blood mononuclear cells of healthy individuals. Journal of General Virology 73, 2463-2466.[CrossRef]
    [Google Scholar]
  21. Sauter, M., Schommer, S., Kremmer, E., Remberger, K., Dolken, G., Lemm, I., Buck, M., Best, B., Neumann-Haefelin, D. & Mueller-Lantzsch, N. ( 1995; ). Human endogenous retrovirus K10: expression of Gag protein and detection of antibodies in patients with seminomas. Journal of Virology 69, 414-421.
    [Google Scholar]
  22. Schommer, S., Sauter, M., Kräusslich, H.-G., Best, B. & Mueller-Lantzsch, N. ( 1996; ). Characterization of the human endogenous retrovirus K proteinase. Journal of General Virology 77, 375-379.[CrossRef]
    [Google Scholar]
  23. Teshima, S., Shimosato, Y., Hirohashi, S., Tome, Y., Hayashi, I., Kanazawa, H. & Kakizoe, T. ( 1988; ). Four new human germ cell tumor cell lines. Laboratory Investigation 59, 328-336.
    [Google Scholar]
  24. T njes, R. R., L wer, R., Boller, K., Denner, J., Hasenmaier, B., Kirsch, H., K nig, H., Korbmacher, C., Limbach, C., Lugert, R., Phelps, R. C., Scherer, J., Thelen, K., L wer, J. & Kurth, R. ( 1996; ). HERV-K: the biologically most active human endogenous retrovirus family. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology 13 (Suppl. 1), S261–267.[CrossRef]
    [Google Scholar]
  25. Tönjes, R. R., Czauderna, F. & Kurth, R. ( 1999; ). Genome-wide screening, cloning, chromosomal assignment, and expression of full-length human endogenous retrovirus type K. Journal of Virology 73, 9187-9195.
    [Google Scholar]
  26. Urnovitz, H. B. & Murphy, W. H. ( 1996; ). Human endogenous retroviruses: nature, occurrence, and clinical implications in human disease. Clinical Microbiology Reviews 9, 72-99.
    [Google Scholar]
  27. Venables, P. J., Brookes, S. M., Griffiths, D., Weiss, R. A. & Boyd, M. T. ( 1995; ). Abundance of an endogenous retroviral envelope protein in placental trophoblasts suggests a biological function. Virology 211, 589-592.[CrossRef]
    [Google Scholar]
  28. Vernon, M. L., McMahon, J. M. & Hackett, J. J. ( 1974; ). Additional evidence of type-C particles in human placentas. Journal of the National Cancer Institute 52, 987-989.
    [Google Scholar]
  29. Zeuthen, J., Norgaard, J. O., Avner, P., Fellous, M., Wartiovaara, J., Vaheri, A., Rosen, A. & Giovanella, B. C. ( 1980; ). Characterization of a human ovarian teratocarcinoma-derived cell line. International Journal of Cancer 25, 19-32.[CrossRef]
    [Google Scholar]
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