1887

Abstract

Rat virus (RV) is an important infectious agent of laboratory rats because of its high prevalence and capacity to disrupt research. Additionally, RV infection serves as a model for characterizing virus–host interactions during acute, persistent and prenatal infection. Our research has examined the pathogenesis of two RV strains, RV-UMass and RV-Y. RV-UMass is more pathogenic, causes a higher level of persistent infection and transmits to the foetus after oronasal inoculation of the pregnant dam. To determine distinctions between the strains that may account for these differences and to provide a benchmark for characterizing virus replication , synchronized replication of both RV strains was defined and compared. The results demonstrated that RV replication has replicative intermediates, virus transcripts and proteins similar to those reported for the prototype parvovirus, minute virus of mice. However, the replicative cycle of RV-UMass was 12 h compared with 24 h for RV-Y, and RV-UMass and RV-Y differed in kinetics of virus DNA replication, transcription and protein accumulation. Additionally, analysis correlated well with kinetics data as determined by Southern and Northern blot analysis. Sequence comparisons between the strains also determined coding differences that may contribute to phenotypic differences.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-3-537
2001-03-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/3/0820537a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-3-537&mimeType=html&fmt=ahah

References

  1. Agbandje-McKenna M., Llamas-Saiz A. L., Wang F., Tattersall P., Rossmann M. G. 1998; Functional implications of the structure of the murine parvovirus, minute virus of mice. Structure 6:1369–1381
    [Google Scholar]
  2. Antonietti J.-P., Sahli R., Beard P., Hirt B. 1988; Characterization of the cell type-specific determinant in the genome of minute virus of mice. Journal of Virology 62:552–557
    [Google Scholar]
  3. Astell C. R., Chow M. B., Ward D. C. 1985; Sequence analysis of the termini of virion and replicative forms of minute virus of mice suggests a modified rolling hairpin model for autonomous parvovirus DNA replication. Journal of Virology 54:171–177
    [Google Scholar]
  4. Astell C. R., Gardiner E. M., Tattersall P. 1986; DNA sequence of the lymphotropic variant of minute virus of mice, MVM(i), and comparison with the DNA sequence of the fibrotropic prototype strain. Journal of Virology 57:656–659
    [Google Scholar]
  5. Ball-Goodrich L. J., Johnson E. 1994; Molecular characterization of a newly recognized mouse parvovirus. Journal of Virology 68:6476–6486
    [Google Scholar]
  6. Ball-Goodrich L. J., Tattersall P. 1992; Two amino acid substitutions within the capsid are co-ordinately required for the acquisition of fibrotropism by the lymphotropic strain of minute virus of mice. Journal of Virology 66:3415–3423
    [Google Scholar]
  7. Ball-Goodrich L. J., Leland S. E., Johnson E. A., Paturzo F. X., Jacoby R. O. 1998; Rat parvovirus type 1: the prototype for a new rodent parvovirus serogroup. Journal of Virology 72:3289–3299
    [Google Scholar]
  8. Brownstein D. G., Smith A. L., Johnson E. A., Pintel D. J., Naeger L. K., Tattersall P. 1992; The pathogenesis of infection with minute virus of mice depends on expression of the small nonstructural protein NS2 and on the genotype of the allotropic determinants VP1 and VP2. Journal of Virology 66:3118–3124
    [Google Scholar]
  9. Chang S.-F., Sgro J.-Y., Parrish C. R. 1992; Multiple amino acids in the capsid structure of canine parvovirus coordinately determine the canine host range and specific antigenic and hemagglutination properties. Journal of Virology 66:6858–6867
    [Google Scholar]
  10. Clemens K. E., Cerutis D. R., Burger L. R., Yang C. Q., Pintel D. J. 1990; Cloning of minute virus of mice cDNAs and preliminary analysis of individual viral proteins expressed in murine cells. Journal of Virology 64:3967–3973
    [Google Scholar]
  11. Cotmore S. F., Tattersall P. 1987; The autonomously replicating parvoviruses of vertebrates. Advances in Virus Research 33:91–174
    [Google Scholar]
  12. Cotmore S. F., Tattersall P. 1989; A genome-linked copy of the NS-1 polypeptide is located on the outside of infectious parvovirus particles. Journal of Virology 63:3902–3911
    [Google Scholar]
  13. Cotmore S. F., Tattersall P. 1990; Alternate splicing in a parvoviral nonstructural gene links a common amino-terminal sequence to downstream domains which confer radically different localization and turnover characteristics. Virology 177:477–487
    [Google Scholar]
  14. Cotmore S. F., Tattersall P. 1996; Parvovirus DNA replication. In DNA Replication in Eukaryotic Cells pp 799–813 Edited by DePamphilis M. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Cotmore S. F., Sturzenbecker L. J., Tattersall P. 1983; The autonomous parvovirus MVM encodes two nonstructural proteins in addition to its capsid polypeptides. Virology 129:333–343
    [Google Scholar]
  16. Cotmore S. F., Gunther M., Tattersall P. 1989; Evidence for a ligation step in the DNA replication of the autonomous parvovirus minute virus of mice. Journal of Virology 63:1002–1006
    [Google Scholar]
  17. Faust E. A., Gloor G. 1984; Characterization of a metastable, partially replicated dimeric intermediate of minute virus of mice. Journal of Virology 49:621–625
    [Google Scholar]
  18. Gaertner D. J., Smith A. L., Jacoby R. O. 1996; Efficient induction of persistent and prenatal parvovirus infection in rats. Virus Research 44:67–78
    [Google Scholar]
  19. Gaillard R. K., Joklik W. K. 1985; The relative translation efficiencies of reovirus messenger RNAs. Virology 147:336–348
    [Google Scholar]
  20. Gardiner E. M., Tattersall P. 1988a; Evidence that developmentally regulated control of gene expression by a parvoviral allotropic determinant is particle mediated. Journal of Virology 62:1713–1722
    [Google Scholar]
  21. Gardiner E. M., Tattersall P. 1988b; Mapping of the fibrotropic and lymphotropic host range determinants of the parvovirus minute virus of mice. Journal of Virology 62:2605–2613
    [Google Scholar]
  22. Hotchin J., Kinch W., Benson L. 1971; Lytic and turbid plaque-type variants of lymphocytic choriomeningitis virus as a cause of neurological disease or persistent infection. Infection and Immunity 4:281–286
    [Google Scholar]
  23. Hotchin J., Kinch W., Benson L., Sikora E. 1975; Role of substrains in persistent lymphocytic choriomeningitis virus infection. Bulletin of the World Health Organization 52:457–463
    [Google Scholar]
  24. Jacoby R. O., Ball-Goodrich L. J. 1995; Parovirus infections of mice and rats. Seminars in Virology 6:329–337
    [Google Scholar]
  25. Jacoby R. O., Gaertner D. J., Bhatt P. N., Paturzo F. X., Smith A. L. 1988; Transmission of experimentally induced rat virus infection. Laboratory Animal Science 38:11–14
    [Google Scholar]
  26. Jacoby R. O., Johnson E. A., Paturzo F. X., Gaertner D. J., Brandsma J. L., Smith A. L. 1991; Persistent rat parvovirus infection in individually housed rats. Archives of Virology 117:193–205
    [Google Scholar]
  27. Jacoby R. O., Johnson E. A., Paturzo F. X., Ball-Goodrich L. 2000; Persistent rat virus infection in smooth muscle of euthymic and athymic rats. Journal of Virology 74:11841–11848
    [Google Scholar]
  28. Legrand C., Rommelaere J., Caillet-Fauquet P. 1993; MVM(p) NS-2 protein expression is required with NS-1 for maximal cytotoxicity in human transformed cells. Virology 195:149–155
    [Google Scholar]
  29. Mitra S., Snyder C. E., Bates R. C., Banerjee P. T. 1982; Comparative physicochemical and biological properties of two strains of Kilham rat virus, a non-defective parvovirus. Journal of General Virology 61:43–54
    [Google Scholar]
  30. Mitra R., Wali T., Vincent V., Fabisch P., Salzman L. A. 1983; Transcription and translation in the autonomous parvovirus KRV. Virology 125:349–360
    [Google Scholar]
  31. Moody M. D., Joklik W. K. 1989; The function of reovirus proteins during the reovirus multiplication cycle: analysis using monoreassortants. Virology 173:437–446
    [Google Scholar]
  32. Paradiso P. R. 1984; Identification of multiple forms of the noncapsid parvovirus protein NCVP1 in H-1 parvovirus-infected cells. Journal of Virology 52:82–87
    [Google Scholar]
  33. Pintel D., Dadachanji D., Astell C. R., Ward D. C. 1983; The genome of minute virus of mice, an autonomous parvovirus, encodes two overlapping transcription units. Nucleic Acids Research 11:1019–1038
    [Google Scholar]
  34. Sahli R., McMaster G. K., Hirt B. 1985; DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice. Nucleic Acids Research 13:3617–3633
    [Google Scholar]
  35. Salzman L. A., Fabisch P. 1978; Studies on the replication of KRV single-stranded linear DNA. Journal of General Virology 39:571–574
    [Google Scholar]
  36. Salzman L. A., Redler B. 1974; Synthesis of viral-specific RNA in cells infected with the parvovirus, Kilham Rat Virus. Journal of Virology 14:434–440
    [Google Scholar]
  37. Salzman L. A., Fabisch P., Mitra R., Wali T. 1982; Synthesis of mRNA by the parvovirus KRV in isolated nuclei. Biken Journal 25:177–183
    [Google Scholar]
  38. Tullis G. E., Burger L. R., Pintel D. J. 1992; The trypsin-sensitive RVER domain in the capsid proteins of minute virus of mice is required for efficient cell binding and viral infection but not for proteolytic processing in vivo . Virology 191:846–857
    [Google Scholar]
  39. Tullis G., Schoborg R. V., Pintel D. J. 1994; Characterization of the temporal accumulation of minute virus of mice replicative intermediates. Journal of General Virology 75:1633–1646
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-3-537
Loading
/content/journal/jgv/10.1099/0022-1317-82-3-537
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error