1887

Abstract

After infection of CEM174.T2 cells [deficient for the transporter of antigen presentation (TAP)] with measles virus (MV) the nucleocapsid protein is recognized by L-restricted cytotoxic T cells in a TAP-independent, chloroquine-sensitive fashion. Presentation via the TAP-independent pathway requires virus replication. During MV infection of the cell the nucleocapsid as well as the matrix protein enter the endolysosomal compartment as indicated by colocalization with the lysosomal-associated membrane protein 1 (LAMP-1). Similarly, the nucleocapsid protein of canine distemper virus (CDV) is recognized in a TAP-independent fashion. In addition, a recombinant MV expressing bacterial β-galactosidase protein is able to introduce the recombinant antigen into the TAP-independent pathway whereas a vaccinia virus expressing β-galactosidase is not. These data and a report about TAP-independent recognition of parainfluenza virus type 1 suggest that members of the family regularly introduce viral proteins into the TAP-independent antigen-processing pathway.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-2-441
2001-02-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/2/0820441a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-2-441&mimeType=html&fmt=ahah

References

  1. Bachmann, M. F., Oxenius, A., Pricher, H., Hengartner, H., Ashton-Richardt, P. A., Tonegawa, S. & Zinkernagel, R. M. ( 1995; ). TAP1-independent loading of class I molecules by exogenous viral proteins. European Journal of Immunology 25, 1739-1743.[CrossRef]
    [Google Scholar]
  2. Beauverger, P., Buckland, R. & Wild, F. T. ( 1993; ). Measles virus antigens induce both type-specific and canine distemper virus cross-reactive cytotoxic T lymphocytes in mice: localization of a common Ld-restricted nucleoprotein epitope. Journal of General Virology 74, 2357-2363.[CrossRef]
    [Google Scholar]
  3. Cathomen, T., Mrkic, B., Spehner, D., Drillien, R., Naef, R., Pavlovic, J., Aguzzi, A., Billeter, M. A. & Cattaneo, R. ( 1998; ). A matrix-less measles virus is infectious and elicits extensive cell fusion: consequences for propagation in the brain. EMBO Journal 17, 3899-3908.[CrossRef]
    [Google Scholar]
  4. Fujihashi, K., McGhee, J. R., Beagley, K. W., McPherson, D. T., McPherson, S. A., Huang, C. M. & Kiyono, H. ( 1993; ). Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells. Journal of Immunological Methods 160, 181-189.[CrossRef]
    [Google Scholar]
  5. Gavin, M. A., Gilbert, M. J., Riddell, S. R., Greenberg, P. D. & Bevan, M. J. ( 1993; ). Alkalis hydrolysis of recombinant proteins allows for the rapid identification of class I MHC-restricted CTL epitopes. Journal of Immunology 151, 3971-3980.
    [Google Scholar]
  6. Gromme, M., Uytdehaag, F. G. C. M., Janssen, H., Calafat, J., van Binnendijk, R. S., Kenter, M. J. H., Tulp, A., Verwoerd, D. & Neefjes, J. ( 1999; ). Recycling MHC class I molecules and endosomal peptide loading. Proceedings of the National Academy of Sciences, USA 96, 10326-10331.[CrossRef]
    [Google Scholar]
  7. Jondal, M., Schirmbeck, R. & Reimann, J. ( 1996; ). MHC class I-restricted CTL responses to exogenous antigens. Immunity 5, 295-302.[CrossRef]
    [Google Scholar]
  8. Kupfer, J. M., Ruan, X. M., Liu, G., Matloff, J., Forrester, J. & Chaux, A. ( 1994; ). High-efficiency gene transfer to autologous rabbit jugular vein grafts using adenovirus-transferrin/polylysine-DNA complexes. Human Gene Therapy 5, 1437-1443.[CrossRef]
    [Google Scholar]
  9. Liu, T., Zhou, X., Örvell, C., Lederer, E., Ljunggren, H.-G. & Jondal, M. ( 1995; ). Heat-inactivated Sendai virus can enter multiple MHC class I processing pathways and generate cytotoxic T lymphocyte responses in vivo. Journal of Immunology 154, 3147-3155.
    [Google Scholar]
  10. Nanan, R., Carstens, C. & Kreth, H. W. ( 1995; ). Demonstration of virus-specific CD8+ memory T cells in measles-seropositive individuals by in vitro peptide stimulation. Clinical and Experimental Immunology 102, 40-45.
    [Google Scholar]
  11. Narang, H. K. ( 1973; ). Electron-microscopic study of measles virus in lymphocytes of affected children. Journal of Hygiene 71, 447-451.[CrossRef]
    [Google Scholar]
  12. Neumeister, C. & Niewiesk, S. ( 1998; ). Recognition of measles virus-infected cells by CD8+ T cells depends on the H-2 molecule. Journal of General Virology 79, 2583-2591.
    [Google Scholar]
  13. Niewiesk, S., Brinckmann, U., Bankamp, B., Sirak, S., Liebert, U. G. & ter Meulen, V. ( 1993; ). Susceptibility to measles virus-induced encephalitis in mice correlates with impaired antigen presentation to cytotoxic T lymphocytes. Journal of Virology 67, 75-81.
    [Google Scholar]
  14. Rammensee, H. G., Schild, H. S. & Theopold, U. ( 1989; ). Protein-specific cytotoxic T lymphocytes. Recognition of transfectants expressing intracellular, membrane-associated or secreted forms of beta-galactosidase. Immunogenetics 30, 296-302.[CrossRef]
    [Google Scholar]
  15. Sarawar, S. R. & Doherty, P. C. ( 1994; ). Concurrent production of interleukin-2, interleukin-10, and gamma interferon in the regional lymph nodes of mice with influenza pneumonia. Journal of Virology 68, 3112-3119.
    [Google Scholar]
  16. Schirmbeck, R. & Reimann, J. ( 1996; ). ′Empty’ Ld molecules capture peptides from endocytosed hepatitis B surface antigen particles for major histocompatibility complex class I restricted presentation. European Journal of Immunology 26, 2812-2822.[CrossRef]
    [Google Scholar]
  17. Schirmbeck, R., Melber, K. & Reimann, J. ( 1995; ). Hepatitis B virus small surface antigen particles are processed in a novel endosomal pathway for major histocompatibility complex class I-restricted epitope presentation. European Journal of Immunology 25, 1063-1070.[CrossRef]
    [Google Scholar]
  18. Schirmbeck, T., Thoma, S. & Reimann, J. ( 1997; ). Processing of exogenous hepatitis B surface antigen particles for Ld-restricted epitope presentation depends on exogenous β2-microglobulin. European Journal of Immunology 27, 3471-3484.[CrossRef]
    [Google Scholar]
  19. Sigal, L. J., Crotty, S., Andino, R. & Rock, K. L. ( 1999; ). Cytotoxic T-cell immunity to virus-infected non-haematopoietic cells requires presentation of exogenous antigen. Nature 398, 77-80.[CrossRef]
    [Google Scholar]
  20. Singh, M., Cattaneo, R. & Billeter, M. A. ( 1999; ). A recombinant measles virus expressing hepatitis B virus surface antigen induces humoral immune responses in genetically modified mice. Journal of Virology 73, 4823-4828.
    [Google Scholar]
  21. ter Meulen, V., Löffler, S., Carter, M. J. & Stephenson, J. R. ( 1981; ). Antigenic characterization of measles and SSPE virus haemagglutinin by monoclonal antibodies. Journal of General Virology 57, 357-364.[CrossRef]
    [Google Scholar]
  22. Zhou, X., Glass, R., Liu, T., Ljunggren, H.-G. & Jondal, M. ( 1993; ). Antigen processing mutant T2 cells present viral antigen restricted through H-2Kb. European Journal of Immunology 23, 1802-1808.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-2-441
Loading
/content/journal/jgv/10.1099/0022-1317-82-2-441
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error