1887

Abstract

The genome of an Australian strain of duck hepatitis B virus (AusDHBV) was cloned from a pool of congenitally DHBV-infected-duck serum, fully sequenced and found by phylogenetic analyses to belong to the ‘Chinese’ DHBV branch of the avian hepadnaviruses. Sequencing of the Pre-S/S gene of four additional AusDHBV clones demonstrated that the original clone (pBL4.8) was representative of the virus present in the pool, and a head-to-tail dimer of the clone was infectious when inoculated into newly hatched ducks. When the published sequences of 20 avian hepadnaviruses were compared, substitutions or deletions in the polymerase (POL) gene were most frequent in the 500 nt segment encoding the ‘spacer’ domain that overlaps with the Pre-S domain of the Pre-S/S gene in a different reading frame. In contrast, substitutions and deletions were rare within the adjacent segment that encodes the reverse transcriptase domain of the POL protein and the S domain of the envelope protein, presumably because they are more often deleterious.

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2001-02-01
2020-01-28
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References

  1. Anderson, D. A., Grgacic, E. V., Luscombe, C. A., Gu, X. & Dixon, R. ( 1997; ). Quantification of infectious duck hepatitis B virus by radioimmunofocus assay. Journal of Medical Virology 52, 354-361.[CrossRef]
    [Google Scholar]
  2. Chang, S. F., Netter, H. J., Bruns, M., Schneider, R., Froelich, K. & Will, H. ( 1999; ). A new avian hepadnavirus infecting snow geese (Anser caerulescens) produces a significant fraction of virions containing single-stranded DNA. Virology 262, 39-54.[CrossRef]
    [Google Scholar]
  3. Huson, D. H. ( 1998; ). Splitstree: analyzing and visualizing evolutionary data. Bioinformatics 14, 68-73.[CrossRef]
    [Google Scholar]
  4. Ishikawa, T. & Ganem, D. ( 1995; ). The pre-S domain of the large viral envelope protein determines host range in avian hepatitis B viruses. Proceedings of the National Academy of Sciences, USA 92, 6259-6263.[CrossRef]
    [Google Scholar]
  5. Jilbert, A. R., Miller, D. S., Scougall, C. A., Turnbull, H. & Burrell, C. J. ( 1996; ). Kinetics of duck hepatitis B virus infection following low dose virus inoculation: one virus DNA genome is infectious in neonatal ducks. Virology 226, 338-345.[CrossRef]
    [Google Scholar]
  6. Kumar, S., Tamura, K. & Nei, M. ( 1994; ). MEGA: molecular evolutionary genetics analysis software for microcomputers. Computer Applications in the Biosciences 10, 189-195.
    [Google Scholar]
  7. Mandart, E., Kay, A. & Galibert, F. ( 1984; ). Nucleotide sequence of a cloned duck hepatitis virus genome: comparison with woodchuck and human hepatitis B virus sequences. Journal of Virology 49, 782-792.
    [Google Scholar]
  8. Mason, W. S., Seal, G. & Summers, J. ( 1980; ). Virus of Pekin ducks with structural and biological relatedness to human hepatitis B virus. Journal of Virology 36, 829-836.
    [Google Scholar]
  9. Mattes, F., Tong, S., Teubner, K. & Blum, H. E. ( 1990; ). Complete nucleotide sequence of a German duck hepatitis B virus. Nucleic Acids Research 18, 6140.[CrossRef]
    [Google Scholar]
  10. Netter, H. J., Chassot, S., Chang, S.-F., Cova, L. & Will, H. ( 1997; ). Sequence heterogeneity of heron hepatitis B virus genomes determined by full-length DNA amplification and direct sequencing reveals novel and unique features. Journal of General Virology 78, 1707-1718.
    [Google Scholar]
  11. Orito, E., Mizokami, M., Ina, Y., Moriyama, E. N., Kameshima, N., Yamamoto, M. & Gojobori, T. ( 1989; ). Host-independent evolution and a genetic classification of the hepadnavirus family based on nucleotide sequences. Proceedings of the National Academy of Sciences, USA 86, 7059-7062.[CrossRef]
    [Google Scholar]
  12. Page, R. D. M. ( 1996; ). TREEVIEW: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 22, 357-358.
    [Google Scholar]
  13. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences, USA 85, 2444-2448.[CrossRef]
    [Google Scholar]
  14. Schlicht, H. J., Salfeld, J. & Schaller, H. ( 1987; ). The duck hepatitis B virus pre-C region encodes a signal sequence which is essential for synthesis and secretion of processed core proteins but not for virus formation. Journal of Virology 61, 3701-3709.
    [Google Scholar]
  15. Sprengel, R., Kuhn, C., Will, H. & Schaller, H. ( 1985; ). Comparative sequence analysis of duck and human hepatitis B virus genome. Journal of Medical Virology 15, 323-333.[CrossRef]
    [Google Scholar]
  16. Sprengel, R., Kaleta, E. F. & Will, H. ( 1988; ). Isolation and characterization of a hepatitis B virus endemic in herons. Journal of Virology 62, 3832-3839.
    [Google Scholar]
  17. Sprengel, R., Schneider, R., Marion, P. L., Fernholz, D., Wildner, G. & Will, H. ( 1991; ). Comparative sequence analysis of defective and infectious avian hepadnaviruses. Nucleic Acids Research 19, 4289.[CrossRef]
    [Google Scholar]
  18. Tagawa, M., Yokosuka, O., Imazeki, F., Ohto, M. & Omata, M. ( 1996; ). Gene expression and active virus replication in the liver after injection of duck hepatitis B virus DNA into the peripheral vein of ducklings. Journal of Hepatology 24, 328-334.[CrossRef]
    [Google Scholar]
  19. Tamura, K. & Nei, M. ( 1993; ). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10, 512-526.
    [Google Scholar]
  20. Tong, S., Mattes, F., Teubner, K. & Blum, H. E. ( 1990; ). Complete nucleotide sequence of a Chinese duck hepatitis B virus (DHBV S18-B). Nucleic Acids Research 18, 6139.[CrossRef]
    [Google Scholar]
  21. Uchida, M., Esumi, M. & Shikata, T. ( 1989; ). Molecular cloning and sequence analysis of duck hepatitis B virus genome of a new variant isolated from Shanghai ducks. Virology 173, 600-606.[CrossRef]
    [Google Scholar]
  22. Wildner, G., Fernholz, D., Sprengel, R., Schneider, R. & Will, H. ( 1991; ). Characterization of infectious and defective cloned avian hepadnavirus genomes. Virology 185, 345-353.[CrossRef]
    [Google Scholar]
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