1887

Abstract

The persistence-associated transcript 1 (PAT1) is actively expressed during persistent infection with Hz-1 virus, while transcription of the rest of the viral genes is shut down. Previously, results of a series deletion of the PAT1 promoter suggested that the regions from nucleotides −312 to −212 and nucleotides −158 to −90 negatively regulate the promoter activity. Here, the negative regulatory effect of the −312/−90 fragment was confirmed using a heterologous IE0 promoter of Autographa californica multiple nucleopolyhedrovirus. Further, the negative regulation of the −312 to −212 region was orientation-independent. The results of electrophoresis mobility shift assays showed that cellular protein(s) bind specifically to DNA fragments −312/−212 and −158/−90. In each of these fragments, a GATA element was identified by computer-assisted analysis. Mutating both GATA elements in the −312/−90 fragment completely eliminated its negative effect on IE0 promoter activity, while mutating only one of these elements had little or no effect. Together, these results suggest that the GATA element has a negative regulatory role on the IE0 and PAT1 promoters.

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2001-02-01
2019-10-20
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References

  1. Amrolia, P. J., Cunninghan, J. M., Ney, P., Nienhuis, A. W. & Jane, S. M. ( 1995; ). Identification of two novel regulatory elements within the 5′-untranslated region of the human Aγ-globin gene. Journal of Biological Chemistry 270, 12892-12898.[CrossRef]
    [Google Scholar]
  2. Chao, Y.-C., Hamblin, M. & Wood, H. A. ( 1990; ). Physical map of Hz-1 baculovirus genome from standard and defective interfering particles. Journal of General Virology 71, 1265-1270.[CrossRef]
    [Google Scholar]
  3. Chao, Y. C., Wood, H. A., Chang, C. Y., Lee, H. J., Shen, W. C. & Lee, H. T. ( 1992; ). Differential expression of Hz-1 baculovirus genes during productive and persistent viral infections. Journal of Virology 66, 1442-1448.
    [Google Scholar]
  4. Chao, Y.-C., Lee, S. T., Chang, M. C., Chen, H. H., Chen, S. S., Wu, T. Y., Liu, F. H., Hsu, E. L. & Hou, R. F. ( 1998; ). A 2·9 kb non-coding nuclear RNA functions in the establishment of persistent Hz-1 viral infection. Journal of Virology 72, 2233-2245.
    [Google Scholar]
  5. Drevet, J. R., Skeiky, Y. A. W. & Iatrous, K. ( 1994; ). GATA type zinc finger motif-containing sequences and chorion gene transcription factors of the silkworm Bombyx mori. Journal of Biological Chemistry 269, 10660-10667.
    [Google Scholar]
  6. Drevet, J. R., Swevers, L. & Iatrous, K. ( 1995; ). Developmental regulation of a silkworm gene encoding multiple GATA-type transcription factors by alternative splicing. Journal of Molecular Biology 246, 43-53.[CrossRef]
    [Google Scholar]
  7. Frumkin, A., Haffiner, R., Shapira, E., Tarcic, N., Gruenbaum, Y. & Fainsod, A. ( 1993; ). The chicken CdxA homeobox gene and axial positioning during gastrulation. Development 118, 553-562.
    [Google Scholar]
  8. Granados, R. R. & Williams, K. A. ( 1986; ). In vivo infection and replication of baculoviruses. In The Biology of Baculoviruses , pp. 159-175. Edited by R. R. Granados & B. A. Federici. Boca Raton:CRC Press.
  9. Granados, R. R., Nguyen, T. & Cato, B. ( 1978; ). An insect cell line persistently infected with a baculovirus-like particle. Intervirology 10, 309-317.[CrossRef]
    [Google Scholar]
  10. Huang, Y. S., Hedberg, M. & Kawanish, C. Y. ( 1982; ). Characterization of the DNA of a nonoccluded baculovirus, Hz-1 V. Journal of Virology 43, 174-181.
    [Google Scholar]
  11. Inoue, N., Dambaugh, T. R., Rapp, J. C. & Pellett, P. E. ( 1994; ). Alphaherpesvirus origin-binding protein homolog encoded by human herpesvirus 6B, a betaherpesvirus, binds to nucleotide sequences that are similar to ori regions of alphaherpesvirus. Journal of Virology 68, 4126-4136.
    [Google Scholar]
  12. Kogan, P. H. & Blissard, G. W. ( 1994; ). A baculovirus gp64 early promoter is activated by host transcription factor binding to CACGTG and GATA elements. Journal of Virology 68, 813-822.
    [Google Scholar]
  13. Kovacs, G. R., Guarino, L. A. & Summers, M. D. ( 1991; ). Novel regulatory properties of the IE1 and IE0 transactivators encoded by the baculovirus Autographa californica multicapsid nuclear polyhedrosis virus. Journal of Virology 65, 5281-5288.
    [Google Scholar]
  14. Krappa, R., Behn-Krappa, A., Jahnel, F., Doerfler, W. & Knebel-Morsdorf, D. ( 1992; ). Differential factor binding at the promoter of early baculovirus gene PE38 during viral infection: GATA motif is recognized by an insect protein. Journal of Virology 66, 3494-3503.
    [Google Scholar]
  15. Lee, S. T., Yu, S. M., Hsu, E. L. & Chao, Y. C. ( 1995; ). Identification of a very early promoter of insect Hz-1 virus using a novel dual-expression shuttle vector. Nucleic Acids Research 23, 4683-4689.[CrossRef]
    [Google Scholar]
  16. Lin, W. H., Huang, L. H., Yeh, J. Y., Lehrach, H., Sun, Y. H. & Tsai, S. F. ( 1995; ). Expression of a Drosophila GATA transcription factor in multiple tissues in the developing embryos. Journal of Biological Chemistry 270, 25150-25158.[CrossRef]
    [Google Scholar]
  17. Lin, C. L., Lee, J. C., Chen, S. S., Wood, H. A., Li, M. L., Li, C. F. & Chao, Y. C. ( 1999; ). Persistent Hz-1 virus infection in insect cells: evidence for insertion of viral DNA into host chromosomes and viral infection in a latent status. Journal of Virology 73, 128-139.
    [Google Scholar]
  18. Lossky, M. & Wensink, P. C. ( 1995; ). Regulation of Drosophila yolk protein genes by an ovary-specific GATA factor. Molecular and Cellular Biology 15, 6943-6952.
    [Google Scholar]
  19. McIntosh, A. H. & Ignoffo, C. M. ( 1981; ). Establishment of a persistent baculovirus infection in a lepidopteran cell line. Journal of Invertebrate Pathology 38, 395-403.[CrossRef]
    [Google Scholar]
  20. Margalit, Y., Yarus, S., Shapira, E., Gruenbaum, Y. & Fainsod, A. ( 1993; ). Isolation and characterization of target sequences of the chicken CdxA homeobox gene. Nucleic Acids Research 21, 4915-4922.[CrossRef]
    [Google Scholar]
  21. Morano, K. A. & Thiele, D. J. ( 1999; ). Heat shock factor function and regulation in response to cellular stress, growth, and differentiation signals. Gene Expression 7, 271-282.
    [Google Scholar]
  22. Nissen, M. S. & Friesen, P. D. ( 1989; ). Molecular analysis of the transcriptional regulatory region of an early baculovirus gene. Journal of Virology 63, 493-503.
    [Google Scholar]
  23. Ogbourne, S. & Antalis, T. M. ( 1999; ). Transcriptional control and the role of silencers in transcriptional regulation in eukaryotes. Biochemistry Journal 331, 1-14.
    [Google Scholar]
  24. Orkin, S. ( 1992; ). GATA-binding transcription factors in hematopoietic cells. Blood 80, 575-581.
    [Google Scholar]
  25. Pullen, S. S. & Friesen, P. D. ( 1995; ). The CAGT motif functions as an initiator element during early transcription of the baculovirus transregulator ie-1. Journal of Virology 69, 3575-3583.
    [Google Scholar]
  26. Raich, N., Clegg, C. H., Grofti, J., Romeo, P. H. & Stamatoyannopoulos, G. ( 1995; ). GATA-1 and YY1 are developmental repressors of the human γ-globin gene. EMBO Journal 14, 801-809.
    [Google Scholar]
  27. Ralston, A. L., Huang, Y. S. & Kawanish, C. Y. ( 1981; ). Cell culture studies with the IMC-Hz-1 nonoccluded virus. Virology 115, 33-44.[CrossRef]
    [Google Scholar]
  28. Ramain, P., Heitzler, P., Haenlin, M. & Simpson, P. ( 1993; ). pannier, a negative regulator of achaete and scute in Drosophila, encodes a zinc finger protein with homology to the vertebrate transcription factor GATA-1. Development 119, 1277-1291.
    [Google Scholar]
  29. Sistonen, L., Sarge, K. D. & Morimoto, R. I. ( 1994; ). Human heat shock factors 1 and 2 are differentially activated and can synergistically induce hsp 70 gene transcription. Molecular and Cellular Biology 14, 2087-2099.
    [Google Scholar]
  30. Turgores, A., Magness, S. T. & Brenner, D. A. ( 1994; ). A single promoter directs both housekeeping and erythroid preferential expression of the human ferrochelatase gene. Journal of Biological Chemistry 269, 30789-30797.
    [Google Scholar]
  31. Vaughn, J. L., Goodwin, R. H., Tompkins, G. J. & McCawley, P. ( 1977; ). The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13, 213-217.[CrossRef]
    [Google Scholar]
  32. Volkman, L. E., Blissard, G. W., Friesen, P. D., Keddie, B. A., Possee, R. D. & Theilmann, D. A. ( 1995; ). Family Baculoviridae. In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses , pp. 104-113. Edited by F. A. Murphy, C. M. Fauquet, D. H. L. Bishop, S. A. Ghabrial, A. W. Javis, G. P. Martelli, M. A. Mayo & M. D. Summers. Vienna & New York:Springer-Verlag.
  33. Yang, H. Y. & Evans, M. T. ( 1995; ). Homotypic interactions of chicken GATA-1 can mediate transcriptional activation. Molecular and Cellular Biology 15, 1353-1363.
    [Google Scholar]
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