1887

Abstract

Previous reports have indicated that () may be essential for the replication of nucleopolyhedrovirus (AcMNPV) because no virus with inactivated was isolated. We have ascertained the requirement for this gene by attempting to inactivate it with a large insertion [the gene encoding GFP (green fluorescent protein)] or by deleting all the conserved domains from the open reading frame (ORF). The gene encoding GFP was inserted into the I site of the ORF and a viral plaque containing the insertion was propagated in SF-21 cells. Similarly, 531 bp (I–I) containing all conserved domains were deleted from the ORF. All mutants were authenticated by PCR amplification, restriction endonuclease analysis, DNA sequencing, and Southern and Northern blot analysis. It was found that inactivation of of AcUW1-LacZ (AcMNPV containing a gene in the locus) had no effect on the biological property of virus, such as virulence and kinetics. These two independent methods showed that is not essential for replication and that this locus could provide another site for the engineering of baculoviruses.

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2001-02-01
2020-01-22
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