1887

Abstract

The complete DNA A of the begomovirus (TbLCZWV) was sequenced: it comprises 2767 nucleotides with six major open reading frames encoding proteins with molecular masses greater than 9 kDa. Full-length TbLCZWV DNA A tandem dimers, cloned in binary vectors (pBin19 and pBI121) and transformed into , were systemically infectious upon agroinoculation of tobacco and tomato. Efforts to identify a DNA B component were unsuccessful. These findings suggest that TbLCZWV is a new member of the monopartite group of begomoviruses. Phylogenetic analysis identified TbLCZWV as a distinct begomovirus with its closest relative being . Abutting primer PCR amplified ca. 1300 bp molecules, and cloning and sequencing of two of these molecules revealed them to be subgenomic defective DNA molecules originating from TbLCZWV DNA A. Variable symptom severity associated with tobacco leaf curl disease and TbLCZWV is discussed.

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2001-12-01
2020-01-26
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References

  1. Berrie, L. C., Rybicki, E. P. & Rey, M. E. C. ( 2001; ). Complete nucleotide sequence and host range of South African cassava mosaic virus: further evidence for recombination amongst begomoviruses. Journal of General of Virology 82, 53-58.
    [Google Scholar]
  2. Bevan, M. ( 1984; ). Binary Agrobacterium vectors for plant transformation. Nucleic Acids Research 12, 711-8721.
    [Google Scholar]
  3. Briddon, R. W., Mansoor, S., Bedford, I. D., Pinner, M. S., Saunders, K., Stanley, J., Zafar, Y., Malik, K. A. & Markham, P. G. ( 2001; ). Identification of DNA components required for induction of cotton leaf curl disease. Virology 285, 234-243.[CrossRef]
    [Google Scholar]
  4. Czosneck, H., Ber, R., Navot, N., Antignus, Y., Cohen, S. & Zamir, D. ( 1989; ). Tomato yellow leaf curl virus DNA forms in the viral capsid, in infected plants and in the insect vector. Journal of Phytopathology 125, 47-54.[CrossRef]
    [Google Scholar]
  5. Doyle, J. J. & Doyle, J. L. ( 1987; ). A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19, 11-15.
    [Google Scholar]
  6. Dry, I. B., Rigden, J. E., Krake, L. R., Mullineaux, P. M. & Rezaian, M. A. ( 1993; ). Nucleotide sequence and genome organization of tomato leaf curl geminivirus. Journal of General Virology 74, 147-151.[CrossRef]
    [Google Scholar]
  7. Fauquet, C. M., Maxwell, D. P., Gronenborn, B. & Stanley, J. ( 2000; ). Revised proposal for naming geminiviruses. Archives of Virology 145, 1743-1761.[CrossRef]
    [Google Scholar]
  8. Frischmuth, T. & Stanley, J. ( 1991; ). African cassava mosaic virus DI DNA interferes with the replication of both genomic components. Virology 183, 539-544.[CrossRef]
    [Google Scholar]
  9. Holsters, M., de Waele, D., Depicker, A., Messens, E., van Montagu, M. & Schell, J. ( 1978; ). Transfection and transformation of Agrobacterium tumefaciens. Molecular and General Genetics 163, 181-187.[CrossRef]
    [Google Scholar]
  10. Idris, A. M. & Brown, J. K. ( 1998; ). Sinaloa tomato leaf curl geminivirus: Biological and molecular evidence for a new subgroup III virus. Phytopathology 88, 648-657.[CrossRef]
    [Google Scholar]
  11. Kheyr-Pour, A., Bendahmane, M., Matzeit, V., Accotto, G. P., Crespi, S. & Gronenborn, B. ( 1991; ). Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted monopartite geminivirus. Nucleic Acids Research 19, 6763-6769.[CrossRef]
    [Google Scholar]
  12. Koncz, C. & Schell, J. ( 1986; ). The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector. Molecular and General Genetics 204, 383-396.[CrossRef]
    [Google Scholar]
  13. Lazarowitz, S. G. ( 1992; ). Geminiviruses: genome structure and gene function. Critical Reviews in Plant Sciences 11(4), 327–349.
    [Google Scholar]
  14. Liu, Y., Robinson, D. J. & Harrison, B. D. ( 1998; ). Defective forms of cotton leaf curl virus DNA-A that have different combinations of sequence deletion, duplication, inversion and rearrangement. Journal of General Virology 79, 1501-1508.
    [Google Scholar]
  15. McClean, A. D. P. ( 1940; ). Some leaf-curl diseases in South Africa. Part I. Leaf-curl disease of tobacco. Science Bulletin, Department of Agriculture and Forestry, South Africa 225, 7-60.
    [Google Scholar]
  16. MacDowell, S. W., Coutts, R. H. A. & Buck, K. W. ( 1986; ). Molecular characterization of subgenomic single-stranded and double-stranded DNA forms isolated from plants infected with tomato golden mosaic virus. Nucleic Acids Research 14, 7967-7984.[CrossRef]
    [Google Scholar]
  17. Navot, N., Pichersky, E., Zeidan, M., Zamir, D. & Czosneck, H. ( 1991; ). Tomato yellow leaf curl virus: a whitefly transmitted geminivirus with a single genomic component. Virology 185, 151-161.[CrossRef]
    [Google Scholar]
  18. Noris, E., Hidalgo, E., Accotto, G. P. & Moriones, E. ( 1994; ). High similarity among the tomato yellow leaf curl virus isolates from the West Mediterranean Basin: the nucleotide sequence of an infectious clone from Spain. Archives of Virology 135, 165-170.[CrossRef]
    [Google Scholar]
  19. Osaki, T. & Inouye, T. (1981). Tobacco leaf curl virus. CMI/AAB Descriptions of Plant Viruses, no. 232.
  20. Paximadis, M. & Rey, M. E. C. ( 1997; ). Aetiology of tobacco leaf curl in southern Africa. Annals of Applied biology 131, 449-457.[CrossRef]
    [Google Scholar]
  21. Paximadis, M., Dusterwald, G., Duyver, L. & Rey, M. E. C. ( 1997; ). Preliminary investigation of the causal agent(s) of a disease causing leaf curl of tobacco in South Africa. Plant Pathology 46, 282-290.[CrossRef]
    [Google Scholar]
  22. Paximadis, M., Idris, A. M., Torres-Jerez, I., Villarreal, A., Rey, M. E. C. & Brown, J. K. ( 1999; ). Characterization of tobacco geminiviruses in the Old and New World. Archives of Virology 144, 703-717.[CrossRef]
    [Google Scholar]
  23. Rey, M. E. C., D’Andrea, E., Calvert-Evers, J., Paximadis, M. & Boccardo, G. ( 1999; ). Evidence for a phytoreovirus associated with tobacco exhibiting leaf curl symptoms in South Africa. Phytopathology 89, 303-307.[CrossRef]
    [Google Scholar]
  24. Rojas, M. R., Gilbertson, R. L., Russel, D. R. & Maxwell, D. P. ( 1993; ). Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Disease 77, 340-347.[CrossRef]
    [Google Scholar]
  25. Rybicki, E. P., Briddon, R. W., Brown, J. K., Fauquet, C. M., Maxwell, D. P., Harrison, B. D., Markham, P. G., Bisaro, D. M., Robinson, D. & Stanley, J. (2000). Family Geminiviridae. In Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses, pp. 285–297. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. San Diego: Academic Press.
  26. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  27. Saunders, K., Bedford, I. D., Briddon, R. W., Markham, P. G., Wong, S. M. & Stanley, J. ( 2000; ). A unique virus complex causes Ageratum yellow vein disease. Proceedings of the National Academy of Sciences, USA 97, 6890-6895.[CrossRef]
    [Google Scholar]
  28. Sharma, A., Murayama, A., Osaki, T. & Ikegami, M. ( 1998; ). Characterization of virus-specific DNA forms from tomato tissues infected by tobacco leaf curl virus: evidence for a single genomic component producing defective DNA molecules. Plant Pathology 47, 787-793.
    [Google Scholar]
  29. Shimizu, S. & Ikegami, M. ( 1999; ). Complete nucleotide sequence and the genome organization of tobacco leaf curl geminivirus from Japan. Microbiology and Immunology 43, 989-992.[CrossRef]
    [Google Scholar]
  30. Stanley, J. & Townsend, R. ( 1985; ). Characterisation of DNA forms associated with cassava latent virus infection. Nucleic Acids Research 13, 2189-2206.[CrossRef]
    [Google Scholar]
  31. Stanley, J., Frischmuth, T. & Ellwood, S. ( 1990; ). Defective viral DNA ameliorates symptoms of geminivirus infection in transgenic plants. Proceedings of the National Academy of Sciences, USA 87, 6291-6295.[CrossRef]
    [Google Scholar]
  32. Stanley, J., Saunders, K., Pinner, M. S. & Wong, S. M. ( 1997; ). Novel defective interfering DNAs associated with Ageratum yellow vein geminivirus infection of Ageratum conyzoides. Virology 239, 87-96.[CrossRef]
    [Google Scholar]
  33. Storey, H. H. ( 1932; ). Leaf curl of tobacco in southern Rhodesia. Rhodesian Agricultural Journal 29, 186-192.
    [Google Scholar]
  34. Wyatt, S. D. & Brown, J. K. ( 1996; ). Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology 86, 1288-1293.[CrossRef]
    [Google Scholar]
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