1887

Abstract

The major structural protein (VP1) of the BK polyomavirus (BKV) was expressed in the recombinant baculovirus expression system. Recombinant BKV VP1 was shown to self-assemble into virus-like particles (VLPs) with a diameter of 45–50 nm. As for other polyomaviruses, BKV VP1 has the capacity to bind to exogenous DNA. Furthermore, the potential of BKV VP1 VLPs was investigated for gene transfer into COS-7 cells using three methods for the formation of pseudo-virions: disassembly/reassembly, osmotic shock and direct interaction between VLPs and reporter plasmid DNA. The latter method was shown to be the most efficient when using linearized plasmid. Gene transfer efficiency with BKV pseudo-virions was of the same order as that observed with human papillomavirus type 16 L1 protein VLPs. In addition, it is demonstrated that cellular entry of BKV pseudo-virions is dependent on cell surface sialic acid.

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2001-12-01
2019-10-16
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References

  1. Arthur, R. R., Shah, K. V., Baust, S. J., Santos, G. W. & Saral, R. ( 1986; ). Association of BK viruria with hemorrhagic cystitis in recipients of bone marrow transplants. New England Journal of Medicine 315, 230-234.[CrossRef]
    [Google Scholar]
  2. Barr, S. M., Keck, K. & Aposhian, H. V. ( 1979; ). Cell-free assembly of a polyoma-like particle from empty capside and DNA. Virology 96, 656-659.[CrossRef]
    [Google Scholar]
  3. Cahan, L. D. & Paulson, J. C. ( 1980; ). Polyoma virus adsorbs to specific sialyloligosaccharide receptors on erythrocytes. Virology 103, 505-509.[CrossRef]
    [Google Scholar]
  4. Chang, D., Cai, X. & Consigli, R. A. ( 1993; ). Characterization of the DNA binding properties of polyomavirus capsid protein. Journal of Virology 67, 6327-6331.
    [Google Scholar]
  5. Chen, M. H. & Benjamin, T. ( 1997; ). Roles of N-glycans with alpha 2,6 as well as alpha 2,3-linked sialic acid in infection by polyomavirus. Virology 233, 440-442.[CrossRef]
    [Google Scholar]
  6. Dhar, R., Lai, C. J. & Khoury, G. ( 1978; ). Nucleotide sequence of the DNA replication origin for human papovavirus BKV: sequence and structural homology with SV40. Cell 13, 345-358.[CrossRef]
    [Google Scholar]
  7. Dörries, K. ( 1998; ). Molecular biology and pathogenesis of human polyomavirus infections. Developments in Biological Standardization 94, 71-79.
    [Google Scholar]
  8. El Mehdaoui, S., Touzé, A., Laurent, S., Sizaret, P. Y., Rasschaert, D. & Coursaget, P. ( 2000; ). Gene transfer using recombinant rabbit hemorrhagic disease virus capsids with genetically modified DNA encapsidation capacity by addition of packaging sequences from L1 or L2 protein of human papillomavirus type 16. Journal of Virology 74, 10332-10340.[CrossRef]
    [Google Scholar]
  9. Flaegstad, T., Andresen, P. A., Johnsen, J. I., Asomano, S. K., Jorgensen, G. E., Vignarajan, S., Kjuul, A., Kogner, P. & Traavik, T. ( 1999; ). A possible contributory role of BK virus infection in neuroblastoma development. Cancer Research 59, 1160-1163.
    [Google Scholar]
  10. Forstova, J., Krauzewicz, N., Sandig, V., Elliot, J., Stauss, M. & Griffin, B. E. ( 1995; ). Polyoma virus pseudocapsids as efficient carriers of heterologous DNA into mammalian cells. Human Gene Therapy 6, 297-306.[CrossRef]
    [Google Scholar]
  11. Fried, H., Cahan, L. & Paulson, J. ( 1981; ). Polyomavirus recognizes specific sialyloligosaccharide receptors on host cells. Virology 109, 188-192.[CrossRef]
    [Google Scholar]
  12. Gillock, E. T., Rottinghaus, S., Chang, D., Cai, X., Smiley, S. A. & Consigli, R. A. ( 1997; ). Polyomavirus major capsid protein VP1 is capable of packaging cellular DNA when expressed in the baculovirus system. Journal of Virology 71, 2857-2865.
    [Google Scholar]
  13. Goldmann, C., Petry, H., Frye, S., Ast, O., Ebitsch, S., Jentsch, K. D., Kaup, F. J., Weber, F., Trebst, C., Nisslein, T., Hunsmann, G., Weber, T. & Luke, W. ( 1999; ). Molecular cloning and expression of major structural protein VP1 of the human polyomavirus JC virus: formation of virus-like particles useful for immunological and therapeutic studies. Journal of Virology 73, 4465-4469.
    [Google Scholar]
  14. Griffith, G. P., Griffith, D. L., Rayment, I., Marakami, W. T. & Caspar, D. L. D. ( 1992; ). Inside polyomavirus at 25 Å resolution. Nature 355, 652-654.[CrossRef]
    [Google Scholar]
  15. Haun, G., Keppler, O. T., Bock, C. T., Herrmann, M., Zentgraf, H. & Pawlita, M. ( 1993; ). The cell surface receptor is a major determinant restricting the host range of the B-Iymphotropic papovavirus. Journal of Virology 67, 7482-7492.
    [Google Scholar]
  16. Ishii, N., Minami, N., Chen, E. Y., Medina, A. L., Chico, M. M. & Kasamatsu, H. ( 1996; ). Analysis of a nuclear localization signal of simian virus 40 major capsid protein Vp1. Journal of Virology 70, 1317-1322.
    [Google Scholar]
  17. Kreiss, P., Cameron, B., Rangara, R., Mailhe, P., Aguerre-Charriol, O., Airiau, M., Scherman, D., Crouzet, J. & Pitard, B. ( 1999; ). Plasmid size does not affect the physicochemical properties of lipoplexes but modulates gene transfer efficiency. Nucleic Acids Research 27, 3792-3798.[CrossRef]
    [Google Scholar]
  18. Lenz, P., Day, P. M., Pang, Y. Y., Frye, S. A., Jensen, P. N., Lowy, D. R. & Schiller, J. T. ( 2001; ). Papillomavirus-like particles induce acute activation of dendritic cells. Journal of Immunology 166, 5346-5355.[CrossRef]
    [Google Scholar]
  19. Liddington, R. C., Yan, Y., Moulai, J., Sahli, R., Benjamin, T. L. & Harrison, S. C. ( 1991; ). Structure of simian virus 40 at 3·8 Å resolution. Nature 354, 278-284.[CrossRef]
    [Google Scholar]
  20. Liu, C. K., Hopa, A. P. & Atwood, W. J. ( 1998; ). The human polyomavirus JCV, does not share receptor specificity with SV40 on human glial cells. Journal of Neurovirology 4, 49-58.[CrossRef]
    [Google Scholar]
  21. Moreland, R. B. & Garcea, R. L. ( 1991; ). Characterization of a nuclear localization sequence in the polyomavirus capsid protein VP1. Virology 185, 513-518.[CrossRef]
    [Google Scholar]
  22. Moreland, R. B., Montross, L. & Garcea, R. L. ( 1991; ). Characterization of the DNA-binding properties of the polyomavirus capsid protein VP1. Journal of Virology 65, 1168-1176.
    [Google Scholar]
  23. Ou, W., Wang, M., Fung, C., Tsai, R., Chao, P., Hseu, T. & Chang, D. ( 1999; ). The major capsid protein, VP1, of human JC virus expressed in Escherichia coli is able to self-assemble into a capsid-like particle and deliver exogenous DNA into human kidney cells. Journal of General Virology 80, 39-46.
    [Google Scholar]
  24. Pawlita, M., Müller, M., Oppenlander, M., Zentgraf, H. & Herrmann, M. ( 1996; ). DNA encapsidation by virus-like particles assembled in insect cells from the major capsid protein VP1 of B-lymphotropic papovavirus. Journal of Virology 70, 7517-7526.
    [Google Scholar]
  25. Roden, R. B. S., Greenstone, H. L., Kirnbauer, R., Booy, F. P., Jessie, J., Lowy, D. R. & Schiller, J. T. ( 1996; ). In vitro generation and type specific neutralization of a human type 16 virion pseudotype. Journal of Virology 70, 5875-5883.
    [Google Scholar]
  26. Sandalon, Z., Dalyot-Herman, N. & Oppenheim, A. ( 1997; ). In vitro assembly of SV40 virions and pseudo-virions: vector for gene therapy. Human Gene Therapy 8, 834-839.
    [Google Scholar]
  27. Schiller, J. T. & Roden, R. B. ( 1995; ). Papillomavirus-like particles. Papillomavirus Report 6, 121-128.
    [Google Scholar]
  28. Seif, I., Khoury, G. & Dhar, R. ( 1979; ). The genome of human papovavirus BK. Cell 18, 963-977.[CrossRef]
    [Google Scholar]
  29. Stokrova, J., Palkova, Z., Fischer, L., Richterova, Z., Korb, J., Griffin, B. E. & Forstova, J. ( 1999; ). Interactions of heterologous DNA with polyomavirus major structural protein, VP1. FEBS Letters 445, 119-125.[CrossRef]
    [Google Scholar]
  30. Touzé, A. & Coursaget, P. ( 1998; ). In vitro gene transfer using human papillomavirus-like particles. Nucleic Acids Research 26, 1317-1323.[CrossRef]
    [Google Scholar]
  31. Touzé, A., El Mehdaoui, S., Sizaret, P. Y., Mougin, C., Muñoz, N. & Coursaget, P. ( 1998; ). The L1 major capsid protein of HPV-16 variants affects the yield of virus-like particles produced in an insect cell expression system. Journal of Clinical Microbiology 36, 2046-2051.
    [Google Scholar]
  32. Unckell, F., Streeck, R. E. & Sapp, M. ( 1997; ). Generation of pseudovirions of human papillomavirus type 33. Journal of Virology 71, 2934-2939.
    [Google Scholar]
  33. Zanta, M. A., Belguise-Valladier, P. & Behr, J. P. ( 1999; ). Gene delivery: a single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus Proceedings of the National Academy of Sciences, USA 96, 91-96.[CrossRef]
    [Google Scholar]
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