1887

Abstract

Full-length DNAs of the Coleman and S7801 strains (pSKY3.0, pSKY5.0) of infectious feline foamy viruses (FFVs) were cloned and sequenced. Parental viruses, designated SKY3.0 and SKY5.0, were secreted following transfection of Crandell feline kidney (CRFK) cells. Production of the rescued parental viruses was enhanced in the presence of trichostatin A. Amino acid sequence similarities between FFV and human foamy virus (HFV) are extremely low for the envelope protein and capsid antigen, as predicted from the two clones. However, a chimeric FFV clone was constructed with the HFV Env substituted for the FFV Env. The chimeric virus (HFFV, SKY4.0) was able to infect and replicate in CRFK cells as well as in peripheral blood mononuclear cells of cats . Consequently, the chimeric HFFV may be useful for the creation of FV vectors for gene transfer strategies.

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/content/journal/jgv/10.1099/0022-1317-82-12-2999
2001-12-01
2024-04-19
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References

  1. Adachi A., Sakai H., Tokunaga K., Kawamura M. 1995; Functional analysis of human spuma retrovirus genome. Virus Genes 11:15–20
    [Google Scholar]
  2. Hatama S., Otake K., Ohta M., Kobayashi M., Imakawa K., Ikemoto A., Okuyama H., Mochizuki M., Miyazawa T., Tohya Y., Fujii Y., Takahashi E. 2001; Reactivation of feline foamy virus from a chronically-infected feline renal cell line by trichostatin A. Virology 283:315–323
    [Google Scholar]
  3. He F., Blair W. S., Fukushima J., Cullen B. R. 1996; The human foamy virus Bel-1 transcription factor is a sequence-specific DNA binding protein. Journal of Virology 70:3902–3908
    [Google Scholar]
  4. Helps C. R., Harbour D. A. 1997; Comparison of the complete sequence of feline spumavirus with those of the primate spumaviruses reveals a shorter gag gene. Journal of General Virology 78:2549–2564
    [Google Scholar]
  5. Hill C. L., Bieniasz P. D., McClure M. O. 1999; Properties of human foamy virus relevant to its development as a vector for gene therapy. Journal of General Virology 80:2003–2009
    [Google Scholar]
  6. Hooks J. J., Gibbs C. J. 1975; The foamy viruses. Bacteriological Reviews 39:169–185
    [Google Scholar]
  7. Ikeda Y., Itagaki S., Tsutsui S., Inoshima Y., Fukasawa M., Tomonaga K., Tohya Y., Maeda K., Mochizuki M., Miyazawa T., Mikami T. 1997; Replication of feline syncytial virus in feline T-lymphoblastoid cells and induction of apoptosis in the cells. Microbiology and Immunology 41:431–435
    [Google Scholar]
  8. Lindemann D., Bock M., Schweizer M., Rethwilm A. 1997; Efficient pseudotyping of murine leukemia virus particles with chimeric human foamy virus envelope proteins. Journal of Virology 71:4815–4820
    [Google Scholar]
  9. Linial M. L. 1999; Foamy viruses are unconventional retroviruses. Journal of Virology 73:1747–1755
    [Google Scholar]
  10. Löchelt M., Zentgraf H., Flügel R. M. 1991; Construction of an infectious DNA clone of the full-length human spumaretrovirus genome and mutagenesis of the bel 1 gene. Virology 184:43–54
    [Google Scholar]
  11. Otake K., Fujii Y., Nakaya T., Nishino Y., Zhong Q., Fujinaga K., Kameoka M., Ohki K., Ikuta K. 1994; The carboxyl-terminal region of HIV-1 Nef protein is a cell surface domain that can interact with CD4+ T cells. Journal of Immunology 153:5826–5837
    [Google Scholar]
  12. Pietschmann T., Heinkelein M., Heldmann M., Zentgraf H., Rethwilm A., Lindemann D. 1999; Foamy virus capsids require the cognate envelope protein for particle export. Journal of Virology 73:2613–2621
    [Google Scholar]
  13. Russell D. W., Miller A. D. 1996; Foamy virus vectors. Journal of Virology 70:217–222
    [Google Scholar]
  14. Winkler I., Bodem J., Haas L., Zemba M., Delius H., Flower R., Flügel R. M., Löchelt M. 1997; Characterization of the genome of feline foamy virus and its proteins shows distinct features different from those of primate spumaviruses. Journal of Virology 71:6727–6741
    [Google Scholar]
  15. Yu S. J., Linial M. L. 1993; Analysis of the role of the bel and bet open reading frames of human foamy virus by using a new quantitative assay. Journal of Virology 67:6618–6624
    [Google Scholar]
  16. Yu S. F., Baldwin D. N., Gwynn S. R., Yendapalli S., Linial M. L. 1996; Human foamy virus replication – a pathway distinct from that of retroviruses and hepadnaviruses. Science 271:1579–1582
    [Google Scholar]
  17. Zemba M., Alke A., Bodem J., Winkler I. G., Flower R. L. P., Pfrepper K.-I., Delius H., Flügel R. M., Löchelt M. 2000; Construction of infectious feline foamy virus genome: cat antisera do not cross-neutralize feline foamy virus chimera with serotype-specific Env sequences. Virology 266:150–156
    [Google Scholar]
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