1887

Abstract

Previous findings indicated that HLA-DR is probably one of the most abundant cellular constituents incorporated within the human immunodeficiency virus type 1 (HIV-1) envelope. Given that the life-cycle of HIV-1 has been reported to be modulated by virion-bound host HLA-DR, an improved version of a virus capture technique was developed to assess the degree of HLA-DR incorporation in several clinical isolates of HIV-1 derived from primary human peripheral blood mononuclear cells (PBMCs) and monocyte-derived macrophages (MDM). Analysis of virus stocks purified from PBMCs and MDM indicated that primary isolates of HIV-1 bearing distinct tropism (i.e. T-, macrophage-, and dual-tropic) all incorporate host cell membrane HLA-DR protein. The amount of incorporated HLA-DR varies among the primary HIV-1 isolates tested. Propagation of some clinical HIV-1 isolates in either autologous PBMCs or MDM resulted in differential incorporation of virion-bound cellular HLA-DR depending on the nature of the virus producer cells. Differences in the degree of HLA-DR incorporation were also noticed when macrophage-tropic isolates of HIV-1 were produced in MDM from different donors. Altogether these data show that the efficiency of HLA-DR incorporation into the envelope of primary isolates of HIV-1 is a multifactorial phenomenon since it is affected by the virus isolate itself, the nature of host cells (i.e. PBMCs or MDM) and the donor source.

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2001-12-01
2020-05-25
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References

  1. Arthur L. O., Bess J. W. J., Sowder R. C.II., Benveniste R. E., Mann D. L., Cherman J.-C., Henderson L. E.. 1992; Cellular proteins bound to immunodeficiency viruses: implication for pathogenesis and vaccines. Science258:1935–1938
    [Google Scholar]
  2. Auger M. J., Ross J. A.. 1992; The biology of the macrophage In The Macrophage: The Natural Immune System. pp1–57 Edited by Lewis C. E., McGee J. O’D. Oxford: IRL Press at Oxford University Press;
  3. Barbeau B., Fortin J.-F., Genois N., Tremblay M. J.. 1998; Modulation of human immunodeficiency virus type 1-induced syncytium formation by the conformational state of LFA-1 determined by a new luciferase-based syncytium quantitative assay. Journal of Virology72:7125–7136
    [Google Scholar]
  4. Blom J., Nielsen C., Rhodes J. M.. 1993; An ultrastructural study of HIV-infected human dendritic cells and monocytes/macrophages. APMIS101:672–680
    [Google Scholar]
  5. Bounou S., Dumais N., Tremblay M. J.. 2001; Attachment of human immunodeficiency virus-1 (HIV-1) particles bearing host-encoded B7-2 proteins leads to nuclear factor-κB- and nuclear factor of activated T cells-dependent activation of HIV-1 long terminal repeat transcription. Journal of Biological Chemistry276:6359–6369
    [Google Scholar]
  6. Bourinbaiar A. S.. 1994; The ratio of defective HIV-1 particles to replication-competent infectious virions. Acta Virologica38:59–61
    [Google Scholar]
  7. Cantin R., Fortin J.-F., Tremblay M.. 1996; The amount of host HLA-DR proteins acquired by HIV-1 is virus strain- and cell type-specific. Virology218:372–381
    [Google Scholar]
  8. Cantin R., Fortin J.-F., Lamontagne G., Tremblay M.. 1997a; The acquisition of host major histocompatibility complex class II glycoproteins by human immunodeficiency virus type 1 accelerates the process of virus entry and infection in human T-lymphoid cells. Blood90:1091–1100
    [Google Scholar]
  9. Cantin R., Fortin J.-F., Lamontagne G., Tremblay M.. 1997b; The presence of host-derived HLA-DR1 on human immunodeficiency virus type 1 increases viral infectivity. Journal of Virology71:1922–1930
    [Google Scholar]
  10. Capobianchi M. R., Fais S., Castilletti C., Gentile M., Ameglio F., Dianzani F.. 1994; A simple and reliable method to detect cell membrane proteins on infectious human immunodeficiency virus type 1 particles. Journal of Infectious Diseases169:886–889
    [Google Scholar]
  11. Fortin J.-F., Cantin R., Lamontagne G., Tremblay M.. 1997; Host-derived ICAM-1 glycoproteins incorporated on human immunodeficiency virus type 1 are biologically active and enhance viral infectivity. Journal of Virology71:3588–3596
    [Google Scholar]
  12. Fortin J.-F., Cantin R., Tremblay M.. 1998; T cells expressing activated LFA-1 are more susceptible to infection with human immunodeficiency virus type 1 particles bearing host-encoded ICAM-1. Journal of Virology72:2105–2112
    [Google Scholar]
  13. Frank I., Stoiber H., Godar S., Stockinger H., Steindl F., Katinger H. W. D., Dierich M. P.. 1996; Acquisition of host cell-surface-derived molecules by HIV-1. AIDS10:1611–1620
    [Google Scholar]
  14. Freed E. O.. 1998; HIV-1 gag proteins: diverse functions in the virus life cycle. Virology251:1–15
    [Google Scholar]
  15. Gay D. P., Maddon P. J., Sékaly R.-P., Talle A., Godfrey M., Long E., Goldstein G., Chess L., Axel R., Kappler J., Marrack P.. 1987; Functional interaction between human T-cell protein CD4 and the major histocompatibility complex HLA-DR antigen. Nature328:626–629
    [Google Scholar]
  16. Gelderblom H. R.. 1991; Assembly and morphology of HIV: potential effect of structure on viral function. AIDS5:617–638
    [Google Scholar]
  17. Guo M. M. L., Hildreth J. E. K.. 1995; HIV acquires functional adhesion receptors from host cells. AIDS Research and Human Retroviruses11:1007–1013
    [Google Scholar]
  18. Henderson L. E., Sowder R., Copeland T. D., Oroszlan S., Arthur L. O., Robey W. G., Fischinger P. J.. 1987; Direct identification of class II histocompatibility DR proteins in preparations of human T-cell lymphotropic virus type III. Journal of Virology61:629–632
    [Google Scholar]
  19. Huby R. D., Dearman R. J., Kimber I.. 1999; Intracellular phosphotyrosine induction by major histocompatibility complex class II requires co-aggregation with membrane rafts. Journal of Biological Chemistry274:22591–22596
    [Google Scholar]
  20. Hwang S. S., Boyle T. J., Lyerly H. K., Cullen B. R.. 1991; Identification of the envelope V3 loop as the primary determinant of cell tropism in HIV-1. Science253:71–74
    [Google Scholar]
  21. Jacobson K., Dietrich C.. 1999; Looking at lipid rafts?. Trends in Cell Biology9:87–91
    [Google Scholar]
  22. Lawn S. D., Roberts B. D., Griffin G. E., Folks T. M., Butera S. T.. 2000; Cellular compartments of human immunodeficiency virus type 1 replication in vivo: determination by presence of virion-associated host proteins and impact of opportunistic infection. Journal of Virology74:139–145
    [Google Scholar]
  23. McKeating J. A., Willey R. L.. 1989; Structure and function of the HIV envelope. AIDS3:s35–s41
    [Google Scholar]
  24. Manie S. N., Debreyne S., Vincent S., Gerlier D.. 2000; Measles virus structural components are enriched into lipid raft microdomains: a potential cellular location for virus assembly. Journal of Virology74:305–311
    [Google Scholar]
  25. Neefjes J.. 1999; CIIV, MIIC and other compartments for MHC class II loading. European Journal of Immunology29:1421–1425
    [Google Scholar]
  26. Nguyen D. H., Hildreth J. E.. 2000; Evidence for budding of human immunodeficiency virus type 1 selectively from glycolipid-enriched membrane lipid rafts. Journal of Virology74:3264–3272
    [Google Scholar]
  27. Orenstein J. M., Meltzer M. S., Phipps T., Gendelman H. E.. 1988; Cytoplasmic assembly and accumulation of human immunodeficiency virus types 1 and 2 in recombinant human colony-stimulating factor-1-treated human monocytes: an ultrastructural study. Journal of Virology62:2578–2586
    [Google Scholar]
  28. Orentas R. J., Hildreth J. E. K.. 1993; Association of host cell surface adhesion receptors and other membrane proteins with HIV and SIV. AIDS Research and Human Retroviruses9:1157–1165
    [Google Scholar]
  29. Paquette J. S., Fortin J. F., Blanchard L., Tremblay M. J.. 1998; Level of ICAM-1 surface expression on virus producer cells influences both the amount of virion-bound host ICAM-1 and human immunodeficiency virus type 1 infectivity. Journal of Virology72:9329–9336
    [Google Scholar]
  30. Poon D. T., Coren L. V., Ott D. E.. 2000; Efficient incorporation of HLA class II onto human immunodeficiency virus type 1 requires envelope glycoprotein packaging. Journal of Virology74:3918–3923
    [Google Scholar]
  31. Roberts B. D., Butera S. T.. 1999; Host protein incorporation is conserved among diverse HIV-1 subtypes. AIDS13:425–427
    [Google Scholar]
  32. Rossio J. L., Bess J., Henderson L. E., Cresswell P., Arthur L. O.. 1995; HLA class II on HIV particles is functional in superantigen presentation to human T cells: implications for HIV pathogenesis. AIDS Research and Human Retroviruses11:1433–1439
    [Google Scholar]
  33. Saarloos M.-N., Sullivan B. L., Czerniewski M. A., Parameswar K. D., Spear G. T.. 1997; Detection of HLA-DR associated with monocytotropic, primary, and plasma isolates of human immunodeficiency virus type 1. Journal of Virology71:1640–1643
    [Google Scholar]
  34. Saifuddin M., Parker C. J., Peeples M. E., Gorny M. K., Zolla-Pazner S., Ghassemi M., Rooney I. A., Atkinson J. P., Spear G. T.. 1995; Role of virion-associated glycosylphosphatidylinositol-linked proteins CD55 and CD59 in complement resistance of cell line-derived and primary isolates of HIV-l. Journal of Experimental Medicine182:501–509
    [Google Scholar]
  35. Tremblay M., Meloche S., Gratton S., Wainberg M. A., Sékaly R. P.. 1994; Association of p56lck with the cytoplasmic domain of CD4 modulates HIV-1 expression. EMBO Journal13:774–783
    [Google Scholar]
  36. Tremblay M. J., Fortin J.-F., Cantin R.. 1998; The acquisition of host-encoded proteins by nascent HIV-1. Immunology Today19:346–351
    [Google Scholar]
  37. Tsai W. P., Kung H. F., Nara P. L.. 1999; The presence and absence of histocompatibility antigens in HIV type 1 produced by autologous blood-derived macrophages and peripheral blood lymphoblasts. AIDS Research and Human Retroviruses15:33–41
    [Google Scholar]
  38. Zhang J., Pekosz A., Lamb R. A.. 2000; Influenza virus assembly and lipid raft microdomains: a role for the cytoplasmic tails of the spike glycoproteins. Journal of Virology74:4634–4644
    [Google Scholar]
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