1887

Abstract

densovirus (DNV-1), on the basis of the previously reported genome sequence, constitutes by itself a separate genus () within the subfamily of parvoviruses. Inconsistencies in the genome organization, however, necessitated its reassessment. The genome sequence of new clones was determined and resulted in a completely different genome organization. The corrected sequence also contained conserved sequence motifs found in other parvoviruses. Some amino acids in the highly conserved domain in the unique region of VP1 were shared by critical amino acids in the catalytic site and Ca-binding loop of secreted phospholipase A, such as from snake and bee venoms. Expression of this domain and determination of enzyme activity demonstrated that capsids have a phospholipase A activity thus far unknown to occur in viruses. This viral phospholipase A, which is required shortly after entry into the cell, showed a substrate preference for phosphatidylethanolamine and phosphatidylcholine over phosphatidylinositol.

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2001-11-01
2020-01-25
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