1887

Abstract

The complete nucleotide sequences are reported of two strains of echovirus 7, the prototype Wallace strain (Eo7-Wallace) and a recent Malaysian strain isolated from the cerebrospinal fluid of a child with fatal encephalomyelitis (Eo7-UMMC strain). The molecular findings corroborate the serological placement of the UMMC strain as echovirus 7. Both Eo7-Wallace and Eo7-UMMC belong to the species human enterovirus B and are most closely related to echovirus 11. Eo7-UMMC has undergone significant genetic drift from the prototype strain in the 47 years that separate the isolation of the two viruses. Phylogenetic analysis revealed that Eo7-UMMC did not arise from recombination with another enterovirus serotype. The molecular basis for the severely neurovirulent phenotype of Eo7-UMMC remains unknown. However, it is shown that mutations in the nucleotide sequence of the 5′ untranslated region (UTR) of Eo7-UMMC result in changes to the putative structure of the 5′ UTR. It is possible that these changes contribute to the neurovirulence of Eo7-UMMC.

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2001-11-01
2019-09-18
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References

  1. Andersson, S. O., Bjorksten, B. & Burman, L. A. ( 1975; ). A comparative study of meningoencephalitis epidemics caused by echovirus type 7 and coxsackievirus type B5. Clinical and virological observations during two epidemics in northern Sweden. Scandinavian Journal of Infectious Diseases 7, 233-237.[CrossRef]
    [Google Scholar]
  2. Bienkowska-Szewczyk, K. & Ehrenfeld, E. ( 1988; ). An internal 5′-noncoding region required for translation of poliovirus RNA in vitro. Journal of Virology 62, 3068-3072.
    [Google Scholar]
  3. Chang, K. H., Auvinen, P., Hyypiä, T. & Stanway, G. ( 1989; ). The nucleotide sequence of coxsackievirus A9; implications for receptor binding and enterovirus classification. Journal of General Virology 70, 3269-3280.[CrossRef]
    [Google Scholar]
  4. Dougherty, R. M. ( 1964; ). Animal virus titration technique. In Techniques in Experimental Virology , pp. 169-224. Edited by R. J. C. Harris. London & New York:Academic Press..
  5. Felsenstein, J. (1993). PHYLIP (Phylogeny Inference Package) version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle, WA, USA.
  6. Higgins, D. G. & Sharp, P. M. ( 1989; ). Fast and sensitive multiple sequence alignments on a microcomputer. Computer Applications in the Biosciences 5, 151-153.
    [Google Scholar]
  7. Ho-Yen, D. O., Hardie, R., McClure, J., Cunningham, N. E. & Bell, E. J. ( 1989; ). Fatal outcome of echovirus 7 infection. Scandinavian Journal of Infectious Diseases 21, 459-461.[CrossRef]
    [Google Scholar]
  8. Iizuka, N., Kuge, S. & Nomoto, A. ( 1987; ). Complete nucleotide sequence of the genome of coxsackievirus B1. Virology 156, 64-73.[CrossRef]
    [Google Scholar]
  9. Inoue, T., Suzuki, T. & Sekiguchi, K. ( 1989; ). The complete nucleotide sequence of swine vesicular disease virus. Journal of General Virology 70, 919-934.[CrossRef]
    [Google Scholar]
  10. Jenkins, O., Booth, J. D., Minor, P. D. & Almond, J. W. ( 1987; ). The complete nucleotide sequence of coxsackievirus B4 and its comparison to other members of the Picornaviridae. Journal of General Virology 68, 1835-1848.[CrossRef]
    [Google Scholar]
  11. Johnson, V. H. & Semler, B. L. ( 1988; ). Defined recombinants of poliovirus and coxsackievirus: sequence-specific deletions and functional substitutions in the 5′-noncoding regions of viral RNAs. Virology 162, 47-57.[CrossRef]
    [Google Scholar]
  12. King, A. M. Q., Brown, F., Christian, P., Hovi, T., Hyypiä, T., Knowles, N. J., Lemon, S. J., Minor, P. D., Palmenberg, A. C., Skern, T. & Stanway, G. ( 2000; ). Family Picornaviridae. In Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses , pp. 657-678. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. San Diego:Academic Press.
  13. Kozak, M. ( 1986; ). Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44, 283-292.[CrossRef]
    [Google Scholar]
  14. Kuge, S. & Nomoto, A. ( 1987; ). Construction of viable deletion and insertion mutants of the Sabin strain of type 1 poliovirus: function of the 5′ noncoding sequence in viral replication. Journal of Virology 61, 1478-1487.
    [Google Scholar]
  15. Liu, C. C., Tseng, H. W., Wang, S. M., Wang, J. R. & Su, I. J. ( 2000; ). An outbreak of enterovirus 71 infection in Taiwan, 1998: epidemiologic and clinical manifestations. Journal of Clinical Virology 17, 23-30.[CrossRef]
    [Google Scholar]
  16. Lum, L. C. S., Wong, K. T., Lam, S. K., Chua, K. B., Goh, A. Y. T., Lim, W. L., Ong, B. B., Paul, G., AbuBakar, S. & Lambert, M. ( 1998a; ). Fatal enterovirus 71 encephalomyelitis. Journal of Pediatrics 133, 795-798.[CrossRef]
    [Google Scholar]
  17. Lum, L. C. S., Wong, K. T., Lam, S. K., Chua, K. B. & Goh, A. Y. T. ( 1998b; ). Neurogenic pulmonary oedema and enterovirus 71 encephalomyelitis. Lancet 352, 1391.
    [Google Scholar]
  18. Lum, L. C. S., Chua, K. B., McMinn, P. C., Goh, A. Y. T., Muridan, R., Sarji, S. A., Hooi, P. S., Chua, B. H. & Lam, S. K. (2001). Echovirus 7-associated encephalomyelitis. Journal of Clinical Virology (in press).
  19. Madhavan, H. N. & Sharma, K. B. ( 1969; ). Enteroviruses from cases of encephalitis in Pondicherry. Indian Journal of Medical Research 57, 1607-1610.
    [Google Scholar]
  20. Mathews, D. H., Sabina, J., Zuker, M. & Turner, D. H. ( 1999; ). Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure. Journal of Molecular Biology 288, 911-940.[CrossRef]
    [Google Scholar]
  21. Melnick, J. L. ( 1996; ). Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In Fields Virology , pp. 655-712. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia:Lippincott–Raven.
  22. Minor, P. D. ( 1992; ). The molecular biology of poliovaccines. Journal of General Virology 73, 3065-3077.[CrossRef]
    [Google Scholar]
  23. Norder, H., Bjerregaard, L. & Magnius, L. O. ( 2001; ). Homotypic echoviruses share aminoterminal VP1 sequence homology applicable for typing. Journal of Medical Virology 63, 35-44.[CrossRef]
    [Google Scholar]
  24. Oberste, M. S., Maher, K., Kilpatrick, D. R. & Pallansch, M. A. ( 1999a; ). Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification. Journal of Virology 73, 1941-1948.
    [Google Scholar]
  25. Oberste, M. S., Maher, K., Kilpatrick, D. R., Flemister, M. R., Brown, B. A. & Pallansch, M. A. ( 1999b; ). Typing of human enteroviruses by partial sequencing of VP1. Journal of Clinical Microbiology 37, 1288-1293.
    [Google Scholar]
  26. Page, R. D. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357-358.
    [Google Scholar]
  27. Pallansch, M. A., Kew, O. M., Semler, B. L., Omilianowski, D. R., Anderson, C. W., Wimmer, E. & Rueckert, R. R. ( 1984; ). Protein processing map of poliovirus. Journal of Virology 49, 873-880.
    [Google Scholar]
  28. Pelletier, J., Kaplan, G., Racaniello, V. R. & Sonenberg, N. ( 1988; ). Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5′ noncoding region. Molecular and Cellular Biology 8, 1103-1112.
    [Google Scholar]
  29. Pilipenko, E. V., Blinov, V. M., Romanova, L. I., Sinyakov, A. N., Maslova, S. V. & Agol, V. I. ( 1989; ). Conserved structural domains in the 5′-untranslated region of picornaviral genomes: an analysis of the segment controlling translation and neurovirulence. Virology 168, 201-209.[CrossRef]
    [Google Scholar]
  30. Pincus, S. E., Diamond, D. C., Emini, E. A. & Wimmer, E. ( 1986; ). Guanidine-selected mutants of poliovirus: mapping of point mutations to polypeptide 2C. Journal of Virology 57, 638-646.
    [Google Scholar]
  31. Pöyry, T., Kinnunen, L., Hyypiä, T., Brown, B., Horsnell, C., Hovi, T. & Stanway, G. ( 1996; ). Genetic and phylogenetic clustering of enteroviruses. Journal of General Virology 77, 1699-1717.[CrossRef]
    [Google Scholar]
  32. Racaniello, V. R. & Meriam, C. ( 1986; ). Poliovirus temperature-sensitive mutants containing a single nucleotide deletion in the 5′-noncoding region of the viral RNA. Virology 155, 498-507.[CrossRef]
    [Google Scholar]
  33. Ramos-Alvaraz, M. & Sabin, A. ( 1954; ). Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proceedings of the Society for Experimental Biology and Medicine 87, 655-661.[CrossRef]
    [Google Scholar]
  34. Seechurn, P., Knowles, N. J. & McCauley, J. W. ( 1990; ). The complete nucleotide sequence of a pathogenic swine vesicular disease virus. Virus Research 16, 255-274.[CrossRef]
    [Google Scholar]
  35. Skinner, M. A., Racaniello, V. R., Dunn, G., Cooper, J., Minor, P. D. & Almond, J. W. ( 1989; ). New model for the secondary structure of the 5′ non-coding RNA of poliovirus is supported by biochemical and genetic data that also show that RNA secondary structure is important in neurovirulence. Journal of Molecular Biology 207, 379-392.[CrossRef]
    [Google Scholar]
  36. Svitkin, Y. V., Maslova, S. V. & Agol, V. I. ( 1985; ). The genomes of attenuated and virulent poliovirus strains differ in their in vitro translation efficiencies. Virology 147, 243-252.[CrossRef]
    [Google Scholar]
  37. Tillett, D., Burns, B. P. & Neilan, B. A. ( 2000; ). Optimized rapid amplification of cDNA ends (RACE) for mapping bacterial mRNA transcripts. Biotechniques 28, 448-456.
    [Google Scholar]
  38. Trono, D., Andino, R. & Baltimore, D. ( 1988a; ). An RNA sequence of hundreds of nucleotides at the 5′ end of poliovirus RNA is involved in allowing viral protein synthesis. Journal of Virology 62, 2291-2299.
    [Google Scholar]
  39. Trono, D., Pelletier, J., Sonenberg, N. & Baltimore, D. ( 1988b; ). Translation in mammalian cells of a gene linked to the poliovirus 5′ noncoding region. Science 241, 445-448.[CrossRef]
    [Google Scholar]
  40. Wreghitt, T. G., Sutehall, G. M., King, A. & Gandy, G. M. ( 1989; ). Fatal echovirus 7 infection during an outbreak in a special care baby unit. Journal of Infection 19, 229-236.[CrossRef]
    [Google Scholar]
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