@article{mbs:/content/journal/jgv/10.1099/0022-1317-82-11-2621, author = "Banerjee, Rajeev and Dasgupta, Asim", title = "Interaction of picornavirus 2C polypeptide with the viral negative-strand RNA", journal= "Journal of General Virology", year = "2001", volume = "82", number = "11", pages = "2621-2627", doi = "https://doi.org/10.1099/0022-1317-82-11-2621", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-82-11-2621", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The picornavirus membrane-associated polypeptide 2C is believed to be required for viral RNA synthesis. Hepatitis A virus (HAV)- and human rhinovirus (HRV)-encoded recombinant 2C proteins have been expressed, purified and examined for their ability to interact with the terminal sequences of viral positive- and negative-strand RNAs. The results demonstrate that both the HAV- and the HRV-encoded 2C polypeptide specifically interact with the 3′-terminal sequences of the negative-strand RNA, but not with the complementary sequences at the 5′ terminus of the positive-strand RNA. This interaction was detected by both mobility gel shift and UV cross-linking assays. Furthermore, complex formation exhibited dose-dependency and competition assays confirmed specificity. These results are consistent with our previous observation using the poliovirus 2C protein. The implication of the picornavirus 2C protein binding to the 3′-terminal sequence of the negative-strand untranslated region in viral RNA synthesis is discussed.", }