1887

Abstract

The complete nucleotide sequence of the blackgram isolate of mungbean yellow mosaic virus, IMYMV-Bg, which infects legumes in India, was determined and compared at the amino acid level with those of other whitefly-transmitted geminiviruses. The genome organization of IMYMV-Bg was similar to that of the begomoviruses. A unique feature of the genome organization was the sequence divergence of the common region (CR) between DNA-A and DNA-B. In order to understand the mechanism of viral DNA replication, the replication initiator protein, Rep, of IMYMV-Bg was overexpressed in . The recombinant and refolded Rep bound to CR-sequences of IMYMV-Bg in a specific manner. In this study, evidence is presented for ATP-upregulated cleavage function and ATP-mediated conformational change of Rep. It is hypothesized that, although ATP is not required for cleavage, ATP-mediated conformational changes may result in better access of Rep to the DNA-cleavage site. Evidence is also presented for a site-specific topoisomerase function of Rep, which has not been demonstrated before. The Rep protein can be classified as a type-I topoisomerase because of its nicking activity and sensitivity towards camptothecin, a topoisomerase type-I inhibitor.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-10-2559
2001-10-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/10/0822559a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-10-2559&mimeType=html&fmt=ahah

References

  1. Arora, D. & Khanna, N. ( 1996; ). Method for increasing the yield of properly folded recombinant human gamma interferon from inclusion bodies. Journal of Biotechnology 52, 127-133.[CrossRef]
    [Google Scholar]
  2. Bisaro, D. M. ( 1996; ). Geminivirus DNA replication. In DNA Replication in Eukaryotic Cells , pp. 833-844. Edited by M. L. DePamphilis. Cold Spring Harbor, NY:Cold Spring Harbor Laboratory.
  3. Desbiez, C., David, C., Mettouchi, A., Laufs, J. & Gronenborn, B. ( 1995; ). Rep protein of tomato yellow leaf curl geminivirus has an ATPase activity required for viral DNA replication. Proceedings of the National Academy of Sciences, USA 92, 5640-5644.[CrossRef]
    [Google Scholar]
  4. Hanley-Bowdoin, L., Settlage, S. B., Orozco, B. M., Nagar, S. & Robertson, D. ( 1999; ). Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation. Critical Reviews in Plant Sciences 18, 71-106.[CrossRef]
    [Google Scholar]
  5. Harrison, B. D. & Robinson, D. J. ( 1999; ). Natural genomic and antigenic variation in whitefly-transmitted geminiviruses (begomoviruses). Annual Review of Phytopathology 37, 369-398.[CrossRef]
    [Google Scholar]
  6. Harrison, B. D., Muniyappa, V., Swanson, M. M., Roberts, I. M. & Robinson, D. J. ( 1991; ). Recognition and differentiation of seven whitefly-transmitted geminiviruses from India, and their relationships to African cassava mosaic and Thailand mung bean yellow mosaic viruses. Annals of Applied Biology 118, 299-308.[CrossRef]
    [Google Scholar]
  7. Hill, J. E., Strandberg, J. O., Hiebert, E. & Lazarowitz, S. G. ( 1998; ). Asymmetric infectivity of pseudorecombinants of cabbage leaf curl virus and squash leaf curl virus: implications for bipartite geminivirus evolution and movement. Virology 250, 283-292.[CrossRef]
    [Google Scholar]
  8. Honda, Y., Iwaki, M., Saito, Y., Thongmeearkom, P., Kittisak, K. & Demma, N. ( 1983; ). Mechanical transmission, purification and some properties of whitefly-borne mungbean yellow mosaic virus in Thailand. Plant Disease 67, 801-804.[CrossRef]
    [Google Scholar]
  9. Hop, D. V., Gaikwad, A., Yadav, B. S., Reddy, M. K., Sopory, S. & Mukherjee, S. K. ( 1999; ). Suppression of pea nuclear topoisomerase I enzyme activity by pea PCNA. Plant Journal 19, 153-162.[CrossRef]
    [Google Scholar]
  10. Laufs, J., Schumacher, S., Geisler, N., Jupin, I. & Gronenborn, B. ( 1995; ). Identification of the nicking tyrosine of geminivirus Rep protein. FEBS Letters 377, 258-262.[CrossRef]
    [Google Scholar]
  11. Lazarowitz, S. G. ( 1992; ). Geminiviruses: genome structure and gene function. Critical Reviews in Plant Sciences 11, 327-349.[CrossRef]
    [Google Scholar]
  12. Mandal, B., Varma, A. & Malathi, V. G. ( 1997; ). Systemic infection of Vigna mungo using the cloned DNAs of the blackgram isolate of mungbean yellow mosaic geminivirus through agroinoculation and transmission of the progeny virus by whiteflies. Journal of Phytopathology 145, 505-510.[CrossRef]
    [Google Scholar]
  13. Morinaga, T., Ikegami, M. & Miura, K. ( 1990; ). Physical mapping and molecular cloning of mung bean yellow mosaic virus DNA. Intervirology 31, 50-56.
    [Google Scholar]
  14. Mukhopadhyay, G. & Chattoraj, D. K. ( 1993; ). Conformation of the origin of P1 plasmid DNA replication. Initiator protein induced wrapping and intrinsic unstacking. Journal of Molecular Biology 231, 19-28.[CrossRef]
    [Google Scholar]
  15. Nariani, T. K. ( 1960; ). Yellow mosaic of mung (Phaseolus aureus L.). Indian Phytopathology 13, 24-29.
    [Google Scholar]
  16. Nene, Y. L. ( 1973; ). Viral disease of some warm weather pulse crops in India. Plant Disease Reporter 57, 463-467.
    [Google Scholar]
  17. Orozco, B. M., Miller, A. B., Settlage, S. B. & Hanley-Bowdoin, L. ( 1997; ). Functional domains of a geminivirus replication protein. Journal of Biological Chemistry 272, 9840-9846.[CrossRef]
    [Google Scholar]
  18. Padidam, M., Beachy, R. N. & Fauquet, C. M. ( 1995; ). Classification and identification of geminiviruses using sequence comparisons. Journal of General Virology 76, 249-263.[CrossRef]
    [Google Scholar]
  19. Rybicki, E. P. ( 1994; ). A phylogenetic and evolutionary justification for three genera of Geminiviridae. Archives of Virology 139, 49-77.[CrossRef]
    [Google Scholar]
  20. Stanley, J. ( 1991; ). The molecular determinants of geminivirus pathogenesis. Seminars in Virology 2, 139-149.
    [Google Scholar]
  21. Stanley, J. ( 1995; ). Analysis of African cassava mosaic virus recombinants suggests strand nicking occurs within the conserved nonanucleotide motif during the initiation of rolling circle DNA replication. Virology 206, 707-712.[CrossRef]
    [Google Scholar]
  22. Stewart, L., Redinbo, M. R., Qiu, X., Hol, W. G. & Champoux, J. J. ( 1998; ). A model for the mechanism of human topoisomerase I. Science 279, 1534-1541.[CrossRef]
    [Google Scholar]
  23. Swanson, M. M., Varma, A., Muniyappa, V. & Harrison, B. D. ( 1992; ). Comparative epitope profiles of the particle proteins of whitefly-transmitted geminiviruses from nine crop legumes in India. Annals of Applied Biology 120, 425-433.[CrossRef]
    [Google Scholar]
  24. Torres-Pacheco, I., Garzón-Tiznado, J. A., Herrera-Estrella, L. & Rivera-Bustamante, R. F. ( 1993; ). Complete nucleotide sequence of pepper huasteco virus: analysis and comparison with bipartite geminiviruses. Journal of General Virology 74, 2225-2231.[CrossRef]
    [Google Scholar]
  25. Vanitha Rani, R., Karthikeyan, A. S., Anuradha, S. & Veluthambi, K. ( 1996; ). Genome homologies among geminiviruses infecting vigna, cassava, acalypha, croton and vernonia. Current Science (Bangalore) 70, 63-69.
    [Google Scholar]
  26. Varma, A., Dhar, A. K. & Mandal, B. ( 1992; ). MYMV transmission and control in India. In Mungbean Yellow Mosaic Disease , pp. 8-27. Edited by S. K. Green & D. Kim. Taipei:Asian Vegetable Research and Development Centre.
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-10-2559
Loading
/content/journal/jgv/10.1099/0022-1317-82-10-2559
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error