1887

Abstract

The 3′ noncoding region (NCR) of the genomic picornaviral RNA is believed to contain major -acting signals required for negative-strand RNA synthesis. The 3′ NCR of foot-and-mouth disease virus (FMDV) was studied in the context of a full-length infectious clone in which the genetic element was deleted or exchanged for the equivalent region of a distantly related swine picornavirus, swine vesicular disease virus (SVDV). Deletion of the 3′ NCR, while maintaining the intact poly(A) tail as well as its replacement for the SVDV counterpart, abrogated virus replication in susceptible cells as determined by infectivity and Northern blot assays. Nevertheless, the presence of the SVDV sequence allowed the synthesis of low amounts of chimeric viral RNA at extended times post-transfection as compared to RNAs harbouring the 3′ NCR deletion. The failure to recover viable viruses or revertants after several passages on susceptible cells suggests that the presence of specific sequences contained within the FMDV 3′ NCR is essential to complete a full replication cycle and that FMDV and SVDV 3′ NCRs are not functionally interchangeable.

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2001-01-01
2020-10-31
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References

  1. Agol V. I., Paul A. V., Wimmer E.. 1999; Paradoxes of the replication of picornaviral genomes. Virus Research62:129–147
    [Google Scholar]
  2. Alexander L., Lu H. H., Wimmer E.. 1994; Polioviruses containing picornavirus type 1 and/or type 2 internal ribosomal entry site elements: genetic hybrids and the expression of a foreign gene. Proceedings of the National Academy of Sciences, USA91:1406–1410
    [Google Scholar]
  3. Bigeriego P., Rosas M. F., Zamora E., Martínez-Salas E., Sobrino F.. 1999; Heterotypic inhibition of foot-and-mouth disease virus infection by combinations of RNA transcripts corresponding to the 5′ and 3′ regions. Antiviral Research44:133–141
    [Google Scholar]
  4. Chatterjee N. K., Polatnick J., Bachrach H. L.. 1976; Cell-free translation of foot-and-mouth disease virus RNA into identifiable non-capsid and capsid proteins. Journal of General Virology32:383–394
    [Google Scholar]
  5. Clarke B. E., Brown A. L., Currey K. M., Newton S. E., Rowlands D. J., Carroll A. R.. 1987; Potential secondary and tertiary structure in the genomic RNA of foot-and-mouth disease virus. Nucleic Acids Research15:7067–7079
    [Google Scholar]
  6. Cui T., Sankar S., Porter A. G.. 1993; Binding of encephalomyocarditis virus RNA polymerase to the 3′-noncoding region of the viral RNA is specific and requires the 3′-poly(A) tail. Journal of Biological Chemistry268:26093–26098
    [Google Scholar]
  7. Falk M. M., Sobrino F., Beck E.. 1992; VPg gene amplification correlates with infective particle formation in foot-and-mouth disease virus. Journal of Virology66:2251–2260
    [Google Scholar]
  8. Gamarnik A. V., Andino R.. 1998; Switch from translation to RNA replication in a positive-stranded RNA virus. Genes & Development12:2293–2304
    [Google Scholar]
  9. Goodfellow I., Chaudhry Y., Richardson A., Meredith J., Almond J. W., Barclay W., Evans D. J.. 2000; Identification of a cis -acting replication element within the poliovirus coding region. Journal of Virology74:4590–4600
    [Google Scholar]
  10. Gutiérrez A., Martínez-Salas E., Pintado B., Sobrino F.. 1994; Specific inhibition of aphthovirus infection by RNAs transcribed from both 5′ and 3′ noncoding region. Journal of Virology68:7426–7432
    [Google Scholar]
  11. Harris K. S., Xiang W., Alexander L., Lane W. S., Paul A. V., Wimmer E.. 1994; Interaction of poliovirus polypeptide 3CDpro with the 5′ and 3′ termini of the poliovirus genome. Identification of viral and cellular co-factors needed for efficient binding. Journal of Biological Chemistry269:27004–27014
    [Google Scholar]
  12. Herold J., Andino R.. 2000a; Functional circularization of a positive-stranded RNA virus: a new model for the initiation of negative strand RNA synthesis in poliovirus replication. Europic 2000 (Zagare Bay, Gargano, Italy, 25–31 May 2000). Abstract G09
    [Google Scholar]
  13. Herold J., Andino R.. 2000b; Poliovirus requires a precise 5′ end for efficient positive-strand RNA synthesis. Journal of Virology74:6394–6400
    [Google Scholar]
  14. Ito T., Lai M. M. C.. 1999; An internal polypyrimidine-tract-binding protein-binding site in the hepatitis C virus RNA attenuates translation, which is relieved by the 3′-untranslated sequence. Virology254:288–296
    [Google Scholar]
  15. Ito T., Tahara S. M., Lai M. M. C.. 1998; The 3′-untranslated region of hepatitis C virus RNA enhances translation from an internal ribosomal entry site. Journal of Virology72:8789–8796
    [Google Scholar]
  16. Kitching R. P.. 1999; Foot-and-mouth disease: current world situation. Vaccine26:1772–1774
    [Google Scholar]
  17. Kühn R., Luz N., Beck E.. 1990; Functional analysis of the internal translation initiation site of foot-and-mouth disease virus. Journal of Virology64:4625–4631
    [Google Scholar]
  18. Kunkel T.. 1985; Rapid and efficient site specific mutagenesis without phenotypic selection. Proceedings of the National Academy of Sciences, USA82:488–492
    [Google Scholar]
  19. Lobert P. E., Escriou N., Ruelle J., Michiels T.. 1999; A coding RNA sequence acts as a replication signal in cardioviruses. Proceedings of the National Academy of Sciences, USA96:11560–11565
    [Google Scholar]
  20. López de Quinto S., Martínez-Salas E.. 1997; Conserved structural motifs located in distal loops of aphthovirus internal ribosome entry site domain 3 are required for internal initiation of translation. Journal of Virology71:4174–4175
    [Google Scholar]
  21. Lu H. H., Wimmer E.. 1996; Poliovirus chimeras replicating under the translational control of genetic elements of hepatitis C virus reveal unusual properties of the internal ribosomal entry site of hepatitis C virus. Proceedings of the National Academy of Sciences, USA93:1412–1417
    [Google Scholar]
  22. McInerney G. M., King A. M., Ross-Smith N., Belsham G. J.. 2000; Replication-competent foot-and-mouth disease virus RNAs lacking capsid coding sequences. Journal of General Virology81:1699–1702
    [Google Scholar]
  23. McKnight K. L., Lemon S. M.. 1998; The rhinovirus type 14 genome contains an internally located RNA structure that is required for viral replication. RNA4:1569–1584
    [Google Scholar]
  24. Mellits K. H., Meredith J. M., Rohll J. B., Evans D. J., Almond J. W.. 1998; Binding of a cellular factor to the 3′ untranslated region of the RNA genomes of entero- and rhinoviruses plays a role in virus replication. Journal of General Virology79:1715–1723
    [Google Scholar]
  25. Paul A. V., Rieder E., Kim D. W., Wimmer E.. 2000; Identification of an RNA hairpin in poliovirus RNA that serves as a specific template for the uridylylation of VPg in vitro. Europic 2000 (Zagare Bay, Gargano, Italy, 25–31 May 2000). Abstract E04
    [Google Scholar]
  26. Pierangeli A., Bucci M., Pagnotti P., Degener A. M., Perez Bercoff R.. 1995; Mutational analysis of the 3’-terminal extra-cistronic region of poliovirus RNA: secondary structure is not the only requirement for minus-strand RNA replication. FEBS Letters374:327–332
    [Google Scholar]
  27. Pilipenko E. V., Maslova S. V., Sinyakov A. N., Agol V. I.. 1992; Towards identification of cis -acting elements involved in the replication of enterovirus and rhinovirus RNAs: a proposal for the existence of tRNA-like terminal structures. Nucleic Acids Research20:1739–1745
    [Google Scholar]
  28. Richards O. C., Ehrenfeld E.. 1990; Poliovirus RNA replication . Current Topics in. Microbiology and Immunology161:89–119
    [Google Scholar]
  29. Rohll J. B., Percy N., Ley R., Evans D. J., Almond J. W., Barclay W. S.. 1994; The 5′-untranslated regions of picornavirus RNAs contain independent functional domains essential for RNA replication and translation. Journal of Virology68:4384–4391
    [Google Scholar]
  30. Rohll J. B., Moon D. H., Evans D. J., Almond J. W.. 1995; The 3′ untranslated region of picornavirus RNA: features required for efficient genome replication. Journal of Virology69:7835–7844
    [Google Scholar]
  31. Rose J. K., Buonocore L., Whitt M. A.. 1991; A new cationic liposome reagent mediating nearly quantitative transfection of animal cells. Biotechniques10:520–525
    [Google Scholar]
  32. Rueckert R. R.. 1990; Picornaviridae and their replication. In Fields Virology pp507–548 Edited by Fields B. N., Knipe D. M.. New York: Raven Press;
    [Google Scholar]
  33. Ryan M. D., Belsham G. J., King A. M.. 1989; Specificity of enzyme-substrate interactions in foot-and-mouth disease virus polyprotein processing. Virology173:35–45
    [Google Scholar]
  34. Sangar D. V., Rowlands D. J., Harris T. J. R., Brown F.. 1977; A protein covalently linked to foot-and-mouth disease virus RNA. Nature268:648–650
    [Google Scholar]
  35. Seechurn P., Knowles N. J., McCauley J. W.. 1990; The nucleotide sequence of a pathogenic swine vesicular disease virus. Virus Research16:255–274
    [Google Scholar]
  36. Sutmoller P.. 1992; Vesicular diseases, foreign animal diseases. In Committee Report on Foreign Animal Diseases of the United States Animal Health Association pp368–382 Richmond, VA, USA:
    [Google Scholar]
  37. Todd S., Semler B. L.. 1996; Structure-infectivity analysis of the human rhinovirus genomic RNA 3′ noncoding region. Nucleic Acids Research24:2133–2142
    [Google Scholar]
  38. Todd S., Nguyen J. H., Semler B. L.. 1995; RNA-protein interactions directed by the 3′ end of human rhinovirus genomic RNA. Journal of Virology69:3605–3614
    [Google Scholar]
  39. Todd S., Towner J. S., Brown D. M., Semler B. L.. 1997; Replication-competent picornavirus with complete genomic RNA 3′ noncoding region deletions. Journal of Virology71:8868–8874
    [Google Scholar]
  40. Waggoner S., Sarnow P.. 1998; Viral ribonucleoprotein complex formation and nucleolar-cytoplasmic relocalization of nucleolin in poliovirus-infected cells. Journal of Virology72:6699–6709
    [Google Scholar]
  41. Zibert A., Maas G., Strebe l. K., Falk M. M., Beck E.. 1990; Infectious foot-and-mouth disease virus derived from a cloned full-length cDNA. Journal of Virology64:2467–2473
    [Google Scholar]
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