1887

Abstract

In , the common strain of the bipartite geminivirus (csTGMV) induces extensive chlorosis whereas the yellow vein strain (yvTGMV) produces veinal chlorosis on systemically infected leaves. In , csTGMV produces leaf distortion and a severe chlorotic mosaic whereas yvTGMV produces only small chlorotic lesions on systemically infected leaves. Genetic recombination and site-directed mutagenesis studies using infectious clones of csTGMV and yvTGMV have identified a role in symptom production for the gene encoding the movement protein (MP). The MP amino acid at position 272, either valine (csTGMV) or isoleucine (yvTGMV), influenced symptoms in both hosts by inducing an intermediate phenotype when exchanged between the two strains. Exchange of an additional strain-specific MP amino acid at position 288, either glutamine (csTGMV) or lysine (yvTGMV), resulted in the change of symptom phenotype to that of the other strain. hybridization analysis in demonstrated that there was no qualitative difference in the tissue distribution of the two strains although csTGMV accumulated in higher amounts, suggesting that the efficiency of virus movement rather than distinct differences in tissue specificity of the strains is responsible for the symptom phenotypes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-1-45
2001-01-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/1/0820045a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-1-45&mimeType=html&fmt=ahah

References

  1. Bisaro D. M. 1996; Geminivirus DNA replication. In DNA Replication in Eukaryotic Cells pp 833–854 Edited by DePamphilis M. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  2. Bradley D., Carpenter R., Sommer H., Hartley N., Coen E. 1993; Complementary floral homeotic phenotypes result from opposite orientations of a transposon at the plena locus of Antirrhinum. Cell 72:85–95
    [Google Scholar]
  3. Duan Y.-P., Powell C. A., Purcifull D. E., Broglio P., Hiebert E. 1997; Phenotypic variation in transgenic tobacco expressing mutated geminivirus movement/pathogenicity (BC1) proteins. Molecular Plant–Microbe Interactions 10:1065–1074
    [Google Scholar]
  4. Gerlach D. 1984; In Botanische Mikrotechnik . , 3rd edn. pp 128–129 Stuttgart & New York: Georg Thieme Verlag;
  5. Haley A., Richardson K., Zhan X., Morris B. 1995; Mutagenesis of the BC1 and BV1 genes of African cassava mosaic virus identifies conserved amino acids that are essential for spread. Journal of General Virology 76:1291–1298
    [Google Scholar]
  6. Hamilton W. D. O., Bisaro D. M., Coutts R. H. A., Buck K. W. 1984; Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO Journal 3:2197–2205
    [Google Scholar]
  7. Hanley-Bowdoin L., Settlage S. B., Orozco B. M., Nagar S., Robertson D. 1999; Geminiviruses: models for replication, transcription, and cell cycle regulation. Critical Reviews in Plant Sciences 18:71–106
    [Google Scholar]
  8. Horns T., Jeske H. 1991; Localization of abutilon mosaic virus (AbMV) DNA within leaf tissue by in situ hybridization. Virology 181:580–588
    [Google Scholar]
  9. Ingham D. J., Pascal E., Lazarowitz S. G. 1995; Both bipartite geminivirus movement proteins define viral host range, but only BL1 determines viral pathogenicity. Virology 207:191–204
    [Google Scholar]
  10. Jackson D. 1992; In situ hybridization in plants. In Molecular Plant Pathology: A Practical Approach . vol 1 pp 163–174 Edited by Gurr S. J., McPherson M. J., Bowles D. J. Oxford: Oxford University Press;
  11. Jeske H., Werz G. 1978; The influence of light intensity on pigment composition and ultrastructure of plastids in leaves of diseased Abutilon sellowianum Reg. Phytopathologische Zeitschrift 91:1–13
    [Google Scholar]
  12. MacDowell S. W., Coutts R. H. A., Buck K. W. 1986; Molecular characterisation of subgenomic single-stranded and double-stranded DNA forms isolated from plants infected with tomato golden mosaic virus. Nucleic Acids Research 14:7967–7984
    [Google Scholar]
  13. Marsh J. L., Erfle M., Wykes E. J. 1984; The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481–485
    [Google Scholar]
  14. Nagar S., Pedersen T. J., Carrick K. M., Hanley-Bowdoin L., Robertson D. 1995; A geminivirus induces expression of a host DNA synthesis protein in terminally differentiated plant cells. Plant Cell 7:705–719
    [Google Scholar]
  15. Noueiry A. O., Lucas W. J., Gilbertson R. L. 1994; Two proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport. Cell 76:925–932
    [Google Scholar]
  16. Pascal E., Goodlove P. E., Wu L. C., Lazarowitz S. G. 1993; Transgenic tobacco plants expressing the geminivirus BL1 protein exhibit symptoms of viral disease. Plant Cell 5:795–807
    [Google Scholar]
  17. Pascal E., Sanderfoot A. A., Ward B. M., Medville R., Turgeon R., Lazarowitz S. G. 1994; The geminivirus BR1 movement protein binds single-stranded DNA and localizes to the cell nucleus. Plant Cell 6:995–1006
    [Google Scholar]
  18. Romeis B. 1989; Mikroskopische Technik . , 17th edn. pp 113–130 Edited by Böck P. Munich, Vienna & Baltimore: Urban und Schwarzenberg;
  19. Rushing A. E., Sunter G., Gardiner W. E., Dute R. R., Bisaro D. M. 1987; Ultrastructural aspects of tomato golden mosaic virus infection in tobacco. Phytopathology 77:1231–1236
    [Google Scholar]
  20. Sanderfoot A. A., Lazarowitz S. G. 1995; Cooperation in viral movement: the geminivirus BL1 movement protein interacts with BR1 and redirects it from the nucleus to the cell periphery. Plant Cell 7:1185–1194
    [Google Scholar]
  21. Sanderfoot A. A., Lazarowitz S. G. 1996; Getting it together in plant virus movement: cooperative interactions between bipartite geminivirus movement proteins. Trends in Cell Biology 6:353–358
    [Google Scholar]
  22. Sanderfoot A. A., Ingham D. J., Lazarowitz S. G. 1996; A viral movement protein as a nuclear shuttle: the geminivirus BR1 movement protein contains domains essential for interaction with BL1 and nuclear localization. Plant Physiology 110:23–33
    [Google Scholar]
  23. Van Regenmortel M. H. V., Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., McGeoch D. J., Maniloff J., Mayo M. A., Pringle C. R., Wickner R. B. (editors) 2000; Virus Taxonomy. Classification and Nomenclature of Viruses. Seventh Report of the International Committee on Taxonomy of Viruses San Diego: Academic Press;
    [Google Scholar]
  24. von Arnim A., Stanley J. 1992; Determinants of tomato golden mosaic virus symptom development located on DNA B. Virology 186:286–293
    [Google Scholar]
  25. Wang H. L., Gilbertson R. L., Lucas W. J. 1996; Spatial and temporal distribution of bean dwarf mosaic geminivirus in Phaseolus vulgaris and Nicotiana benthamiana . Phytopathology 86:1204–1214
    [Google Scholar]
  26. Ward B. M., Medville R., Lazarowitz S. G., Turgeon R. 1997; The geminivirus BL1 movement protein is associated with endoplasmic reticulum-derived tubules in developing phloem cells. Journal of Virology 71:3726–3733
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-82-1-45
Loading
/content/journal/jgv/10.1099/0022-1317-82-1-45
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error