1887

Abstract

NS3 protein sequences of recent African horsesickness virus (AHSV) field isolates, reference strains and current vaccine strains in southern Africa were determined and compared. The variation of AHSV NS3 was found to be as much as 36·3% across serotypes and 27·6% within serotypes. NS3 proteins of vaccine and field isolates of a specific serotype were found to differ between 2·3% and 9·7%. NS3 of field isolates within a serotype differed up to 11·1%. Our data indicate that AHSV NS3 is the second most variable AHSV protein, the most variable being the major outer capsid protein, VP2. The inferred phylogeny of AHSV NS3 corresponded well with the described NS3 phylogenetic clusters. The only exception was AHSV-8 NS3, which clustered into different groups than previously described. No obvious sequence markers could be correlated with virulence. Our results suggest that NS3 sequence variation data could be used to distinguish between field isolates and live attenuated vaccine strains of the same serotype.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-1-149
2001-01-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/1/0820149a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-1-149&mimeType=html&fmt=ahah

References

  1. Barnard, B. J. H. ( 1993; ). Circulation of African horse sickness virus in zebra (Equus burchelli) in the Kruger National Park, South Africa, as measured by the prevalence of type specific antibodies. Onderstepoort Journal of Veterinary Research 60, 111-117.
    [Google Scholar]
  2. Bonneau, K. R., Zhang, N., Zhu, J., Zhang, F., Li, Z., Zhang, K., Xiao, L., Xiang, W. & MacLachlan, N. J. ( 1999; ). Sequence comparison of the L2 and S10 genes of bluetongue viruses from the United States and the People’s Republic of China. Virus Research 61, 153-160.[CrossRef]
    [Google Scholar]
  3. Ciarlet, M., Liprandi, F., Conner, M. E. & Estes, M. K. ( 2000; ). Species specificity and interspecies relatedness of NSP4 genetic groups by comparative NSP4 sequence analyses of animal rotaviruses. Archives of Virology 145, 371-383.[CrossRef]
    [Google Scholar]
  4. Coetzer, J. A. & Erasmus, B. J. ( 1994; ). African horsesickness. In Infectious Disease of Livestock with Special Reference to Southern Africa, vol. 1 , pp. 460-475. Edited by J. A. Coetzer, C. R. Thomson& R. C. Tustin. Oxford:Oxford University Press.
  5. Howell, P. G. ( 1962; ). The isolation and identification of further antigenic types of African horsesickness virus. Onderstepoort Journal of Veterinary Research 29, 139-147.
    [Google Scholar]
  6. Hwang, G., Yang, Y., Chiou, J. & Li, J. K. ( 1992; ). Sequence conservation among the cognate nonstructural NS3/NS3A protein genes of six bluetongue viruses. Virus Research 23, 151-161.[CrossRef]
    [Google Scholar]
  7. Hyatt, A. D., Zhao, Y. & Roy, P. ( 1993; ). Release of bluetongue virus-like particles from insect cells is mediated by BTV nonstructural protein NS3/NS3A. Virology 193, 592-603.[CrossRef]
    [Google Scholar]
  8. Jensen, M. J. & Wilson, W. C. ( 1995; ). A model for the membrane topology of the NS3 protein as predicted from the sequence of segment 10 of epizootic haemorrhagic disease virus serotype 1. Archives of Virology 140, 799-805.[CrossRef]
    [Google Scholar]
  9. Kirkwood, C. D. & Palombo, E. A. ( 1997; ). Genetic characterization of the rotavirus nonstructural protein, NSP4. Virology 236, 258-265.[CrossRef]
    [Google Scholar]
  10. Laegreid, W., Skowronek, W. A., Stone-Marschat, M. & Burrage, T. ( 1993; ). Characterization of virulence variants of African horsesickness virus. Virology 195, 836-839.[CrossRef]
    [Google Scholar]
  11. Lubroth, J. ( 1988; ). African horsesickness and the epidemiology in Spain. Equine Practice 10, 26-33.
    [Google Scholar]
  12. McIntosh, B. M. ( 1956; ). Complement fixation with horsesickness virus. Onderstepoort Journal of Veterinary Research 27, 165-169.
    [Google Scholar]
  13. McLaughlin, S. & Aderem, A. ( 1995; ). The myristoyl-electrostatic switch: a modulator of reversible protein–membrane interaction. Trends in Biochemical Sciences 20, 272-275.[CrossRef]
    [Google Scholar]
  14. Martin, L. A. & Meyer, A. J. ( 1998; ). Phylogenetic analysis of African horse sickness virus segment 10: sequence variation, virulence characteristics and cell exit. Archives of Virology Supplement 14, 281-293.
    [Google Scholar]
  15. Mellor, P. S. ( 1993; ). African horsesickness: transmission and epidemiology. Veterinary Research 24, 199-212.
    [Google Scholar]
  16. O’Hara, R. S., Meyer, A. J., Burroughs, J. N., Pullen, L., Martin, L. A. & Mertens, P. P. ( 1998; ). Development of a mouse model system, coding assignments and identification of the genome segments controlling virulence of African horse sickness virus serotypes 3 and 8. Archives of Virology Supplement 14, 259-279.
    [Google Scholar]
  17. Pierce, C. M., Balasuriya, U. B. & Maclachlan, N. J. ( 1998; ). Phylogenetic analysis of the S10 gene of field and laboratory strains of bluetongue virus from the United States. Virus Research 55, 15-27.[CrossRef]
    [Google Scholar]
  18. Roy, P., Mertens, P. P. & Cascal, I. ( 1994; ). African horse sickness virus structure. Comparative Immunology Microbiology and Infectious Diseases 17, 243-273.[CrossRef]
    [Google Scholar]
  19. Sailleau, C., Moulay, S. & Zientara, S. ( 1997; ). Nucleotide sequence comparisons of the segment s10 of the nine African horsesickness virus serotypes. Archives of Virology 14, 965-978.
    [Google Scholar]
  20. Silverman, L. & Resh, M. D. ( 1992; ). Lysine residues form an integral component of a novel NH2-terminal membrane targeting motif for myristylated pp60v-src. Journal of Cellular Biology 119, 415-425.[CrossRef]
    [Google Scholar]
  21. Stoltz, M. A., van der Merwe, C. F., Coetzee, J. & Huismans, H. ( 1996; ). Subcellular localization of the nonstructural protein NS3 of African horsesickness virus. Onderstepoort Journal of Veterinary Research 63, 57-61.
    [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876-4882.[CrossRef]
    [Google Scholar]
  23. Tian, P., Ottaiano, A., Reilly, P. A., Udem, S. & Zamb, T. ( 2000; ). The authentic sequence of rotavirus SA11 nonstructural protein NSP4. Virus Research 66, 117-122.[CrossRef]
    [Google Scholar]
  24. Van Staden, V. & Huismans, H. ( 1991; ). A comparison of the genes which encode nonstructural protein NS3 of different orbiviruses. Journal of General Virology 72, 1073-1079.[CrossRef]
    [Google Scholar]
  25. Van Staden, V., Stoltz, M. A. & Huismans, H. ( 1995; ). Expression of nonstructural protein NS3 of African horsesickness virus (AHSV): evidence for a cytotoxic effect of NS3 in insect cells, and characterization of the gene products in AHSV infected Vero cells. Archives of Virology 140, 289-306.[CrossRef]
    [Google Scholar]
  26. Van Staden, V., Smit, C. C., Stoltz, M. A., Maree, F. F. & Huismans, H. ( 1998; ). Characterization of two African horse sickness virus nonstructural proteins, NS1 and NS3. Archives of Virology Supplement 14, 251-258.
    [Google Scholar]
  27. Wade-Evans, A. M. ( 1990; ). Complete sequence of genome segment 10, encoding the NS3 protein, of bluetongue virus, serotype 1 from South Africa. Nucleic Acids Research 18, 4920.[CrossRef]
    [Google Scholar]
  28. Zhang, M., Zeng, C. Q.-Y., Dong, Y., Ball, J. M., Saif, L. J., Morris, A. P. & Estes, K. M. ( 1998; ). Mutations in rotavirus nonstructural glycoprotein NSP4 are associated with altered virus virulence. Journal of Virology 72, 3666-3672.
    [Google Scholar]
  29. Zhou, W., Parent, L. J., Wills, J. W. & Resh, M. D. ( 1994; ). Identification of a membrane-binding domain within the amino terminal region of human immunodeficiency virus type 1 Gag protein which interacts with acidic phospholipids. Journal of Virology 68, 2556-2569.
    [Google Scholar]
  30. Zientara, S., Sailleau, C., Plateau, E., Moulay, S., Mertens, P. P. & Cruciere, C. ( 1998; ). Molecular epidemiology of African horse sickness virus based on analyses and comparisons of genome segments 7 and 10. Archives of Virology Supplement 14, 221-234.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-1-149
Loading
/content/journal/jgv/10.1099/0022-1317-82-1-149
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error