1887

Abstract

Cucumber vein yellowing virus (CVYV) is widespread in cucurbits in the Middle East. CVYV has filamentous particles and is transmitted by by the semi-persistent mode. It has not yet been assigned to a specific genus or family. Ultramicroscopic observations revealed numerous cylindrical cytoplasmic inclusions in melon and cucumber cells infected by CVYV isolates from Israel and Jordan. Depending on the section orientation, the inclusions appeared as pinwheels or as bundles. In addition, a 1·9 kb DNA fragment was amplified by RT–PCR from CVYV-infected plant extracts using primers designed to detect all potyvirids. Sequence comparisons with the amplified fragment indicated that CVYV is more closely related to than to any other virus in the family . These results suggest that CVYV can be considered as a tentative new member of the genus , family .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-9-2289
2000-09-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/9/0812289a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-9-2289&mimeType=html&fmt=ahah

References

  1. Al-Musa A. M., Qusus S. J., Mansour A. N. 1985; Cucumber vein yellowing virus on cucumber in Jordan. Plant Disease 69:361
    [Google Scholar]
  2. Brunt A., Crabtree K., Dallwitz M., Gibbs A., Watson L. 1996 Viruses of Plants. Description and Lists from the VIDE Database Wallingford, UK: CAB International;
  3. Cohen S., Nitzany F. E. 1960; A whitefly transmitted virus of cucurbits in Israel. Phytopathologia Mediterranea 1:44–46
    [Google Scholar]
  4. Delécolle B. 1978; Essais de rationalisation des méthodes de préparation d’échantillons végétaux pour la microscopie électronique: problème des précipités parasites. Cellular and Molecular Biology 23:431–436
    [Google Scholar]
  5. Edwardson J. R., Christie R. G. 1996; Cylindrical Inclusions . Bulletin 894 Gainesville, FL: University of Florida Agricultural Experiment Station;
    [Google Scholar]
  6. Felsenstein J. 1989; PHYLIP – Phylogeny Inference Package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  7. Gibbs A., Mackenzie A. 1997; A primer pair for amplifying part of the genome of all potyvirids by RT–PCR. Journal of Virological Methods 63:9–16
    [Google Scholar]
  8. Harpaz I., Cohen S. 1965; Semipersistent relationship between cucumber vein yellowing virus (CVYV) and its vector, the tobacco whitefly ( Bemisia tabaci Gennadius). Phytopathologische Zeitschrift 54:240–248
    [Google Scholar]
  9. Jacquet C., Delecolle B., Raccah B., Lecoq H., Dunez J., Ravelonandro M. 1998; Use of modified plum pox virus coat protein genes developed to limit heteroencapsidation-associated risks in transgenic plants. Journal of General Virology 79:1509–1517
    [Google Scholar]
  10. Jordan R. 1992; Potyviruses, monoclonal antibodies, and antigenic sites. Archives of Virology Supplementum 5:81–95
    [Google Scholar]
  11. Lecoq H., Wisler G., Pitrat M. 1998; Cucurbit viruses: the classics and the emerging. In Cucurbitaceae ’98. Evaluation and Enhancement of Cucurbit Germplasm pp 126–142 Edited by McCreight J. D. Alexandria, VA: ASHS Press;
    [Google Scholar]
  12. Mansour A., Al-Musa A. 1993; Cucumber vein yellowing virus; host range and virus vector relationships. Journal of Phytopathology 137:73–78
    [Google Scholar]
  13. Mansour A. N., Hadidi N. 1999; Cucumber vein yellowing virus: purification and serological studies. Dirasat Agricultural Sciences 26:8–14
    [Google Scholar]
  14. Page R. D. M. 1996; TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12:357–358
    [Google Scholar]
  15. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences, USA 85:2444–2448
    [Google Scholar]
  16. Pringle C. R. 1999; Virus Taxonomy –; 1999; The universal system of virus taxonomy, updated to include the new proposals ratified by the International Committee on Taxonomy of Viruses during 1998. Archives of Virology 144:421–429
    [Google Scholar]
  17. Richter J., Rabenstein F., Proll E., Vetten H. J. 1995; Use of cross-reactive antibodies to detect members of the Potyviridae . Journal of Phytopathology 143:459–464
    [Google Scholar]
  18. Sela I., Assouline I., Tanne E., Cohen S., Marco S. 1980; Isolation and characterization of a rod-shaped, whitefly-transmissible, DNA- containing plant virus. Phytopathology 70:226–228
    [Google Scholar]
  19. Shukla D. D., Ward C. W., Brunt A. A. 1994 The Potyviridae Wallingford, UK: CAB International;
  20. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680
    [Google Scholar]
  21. Yilmaz M. A., Ozaslan M., Ozaslan D. 1989; Cucumber vein yellowing virus in Cucurbitaceae in Turkey. Plant Disease 73:610
    [Google Scholar]
  22. Zouba A. A., Lopez M. V., Anger H. 1998; Squash yellow leaf curl virus: a new whitefly-transmitted poty-like virus. Plant Disease 82:475–478
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-9-2289
Loading
/content/journal/jgv/10.1099/0022-1317-81-9-2289
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error