1887

Abstract

A novel TT virus (TTV)-like DNA sequence was detected in the serum of a patient (PM) with acute non-A–E hepatitis. The full-length genome sequence, referred to here as PM virus (PMV), was obtained and its relationship to other full or near full-length TTV sequences examined. Although it shares a common genomic arrangement and short conserved regions, the majority of the genome is extremely divergent, displaying an average genetic distance of 0·60 from all other TTV sequences. By comparing PMV with TTV genomes representing the most divergent types so far described, six major groups can be distinguished. The level of genetic diversity seen between these genomes is higher than would be expected within a single virus species. Indeed, PMV could be considered the prototype of an independent taxonomic group within the family. A genoprevalence study of sera from blood donors and patients with acute hepatitis suggests that PMV is rare.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-9-2273
2000-09-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/9/0812273a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-9-2273&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25:3389–3402
    [Google Scholar]
  2. Boom R., Sol C. J. A., Salimans M. M. M., Jansen C. L., Wertheim-van Dillen P. M. E., van der Noordaa J. 1990; Rapid and simple method for purification of nucleic acids. Journal of Clinical Microbiology 28:495–503
    [Google Scholar]
  3. Coombs N. J., Gough A. C., Primrose J. N. 1999; Optimization of DNA and RNA extraction from archival formalin-fixed tissue. Nucleic Acids Research 27:12–14
    [Google Scholar]
  4. Erker J. C., Leary T. P., Desai S. M., Chalmers M. L., Mushahwar I. K. 1999; Analyses of TT virus full-length genomic sequences. Journal of General Virology 80:1743–1750
    [Google Scholar]
  5. Felsenstein J. 1993 PHYLIP (phylogeny inference package) v3.5c Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  6. Hijikata M., Takahashi K., Mishiro S. 1999; Complete circular DNA genome of a TT virus variant (isolate name SANBAN) and 44 partial ORF2 sequences implicating a great degree of diversity beyond genotypes. Virology 260:17–22
    [Google Scholar]
  7. Jaeger J. A., Turner D. H., Zuker M. 1989; Improved predictions of secondary structures for RNA. Proceedings of the National Academy of Sciences, USA 86:7706–7710
    [Google Scholar]
  8. Kozak M. 1986; Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:283–292
    [Google Scholar]
  9. Leary T. P., Erker J. C., Chalmers M. L., Desai S. M., Mushahwar I. K. 1999; Improved detection systems for TT virus reveal high prevalence in humans, non-human primates and farm animals. Journal of General Virology 80:2115–2120
    [Google Scholar]
  10. Miyata H., Tsunoda H., Kazi A., Yamada A., Khan M. A., Murakami J., Kamahora T., Shiraki K., Hino S. 1999; Identification of a novel GC-rich 113-nucleotide region to complete the circular, single-stranded DNA genome of TT virus, the first human circovirus. Journal of Virology 73:3582–3586
    [Google Scholar]
  11. Morozov I., Sirinarumitr T., Sorden S. D., Halbur P. G., Morgan M. K., Yoon K.-J., Paul P. S. 1998; Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. Journal of Clinical Microbiology 36:2535–2541
    [Google Scholar]
  12. Mulyanto M., Hijikata M., Matsushita M., Ingkokusmo G., Widjaya A., Sumarsidi D., Kanai K., Ohta Y., Mishiro S. 2000; TT virus (TTV) genotypes in native and non-native prostitutes of Irian Jaya, Indonesia: implication for non-occupational transmission. Archives of Virology 145:63–72
    [Google Scholar]
  13. Mushahwar I. K., Erker J. C., Muerhoff A. S., Leary T. P., Simons J. N., Birkenmeyer L. G., Chalmers M. L., Pilot-Matias T. J., Desai S. M. 1999; Molecular and biophysical characterization of TT virus: evidence for a new virus family infecting humans. Proceedings of the National Academy of Sciences, USA 96:3177–3182
    [Google Scholar]
  14. Nishizawa T., Okamoto H., Konishi K., Yoshizawa H., Miyakawa Y., Mayumi M. 1997; A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology. Biochemical and Biophysical Research Communications 241:92–97
    [Google Scholar]
  15. Nishizawa T., Okamoto H., Tsuda F., Aikawa T., Sugai Y., Konishi K., Akahane Y., Ukita M., Tanaka T., Miyakawa Y., Mayumi M. 1999; Quasispecies of TT virus (TTV) with sequence divergence in hypervariable regions of the capsid protein in chronic TTV infection. Journal of Virology 73:9604–9608
    [Google Scholar]
  16. Okamoto H., Akahane Y., Ukita M., Fukuda M., Tsuda F., Miyakawa Y., Mayumi M. 1998; Fecal excretion of a nonenveloped DNA virus (TTV) associated with posttransfusion non A-G hepatitis. Journal of Medical Virology 56:128–132
    [Google Scholar]
  17. Okamoto H., Takahashi M., Nishizawa T., Ukita M., Fukuda M., Tsuda F., Miyakawa Y., Mayumi M. 1999a; Marked heterogeneity and frequent mixed infection of TT virus demonstrated by PCR with primers from coding and noncoding regions. Virology 259:428–436
    [Google Scholar]
  18. Okamoto H., Nishizawa T., Ukita M., Takahashi M., Fukuda M., Iizuka H., Miyakawa Y., Mayumi M. 1999b; The entire nucleotide sequence of a TT virus isolate from the United States (TUS01): comparison with reported isolates and phylogenetic analysis. Virology 259:437–448
    [Google Scholar]
  19. Page R. D. M. 1996; Treeview: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12:357–358
    [Google Scholar]
  20. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences, USA 85:2444–2448
    [Google Scholar]
  21. Pringle C. L. 1999; Virus taxonomy at the XIth International Congress of Virology, Sydney, Australia, 1999. Archives of Virology 144:2065–2068
    [Google Scholar]
  22. Takahashi K., Ohta Y., Mishiro S. 1998a; Partial ∼2·4-kb sequences of TT virus (TTV) genome from eight Japanese isolates: diagnostic and phylogenetic implications. Hepatology Research 12:111–120
    [Google Scholar]
  23. Takahashi K., Hoshino H., Ohta Y., Yoshida N., Mishiro S. 1998b; Very high prevalence of TT virus (TTV) infection in general population of Japan revealed by a new set of PCR primers. Hepatology Research 12:233–239
    [Google Scholar]
  24. Takahashi K., Iwasa Y., Hijikata M., Mishiro S. 2000; Identification of a new human DNA virus (TTV-like mini virus: TLMV) intermediately related to TT virus and chicken anaemia virus. Archives of Virology (in press
    [Google Scholar]
  25. Tanaka Y., Mizokami M., Orito E., Ohno T., Nakano T., Kato T., Kato H., Mukaide M., Park Y.-M., Kim B.-S., Ueda R. 1998; New genotypes of TT virus (TTV) and a genotyping assay based on restriction fragment length polymorphism. FEBS Letters 437:201–206
    [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-9-2273
Loading
/content/journal/jgv/10.1099/0022-1317-81-9-2273
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error