1887

Abstract

Previous studies have shown that infection of human fibroblasts with human cytomegalovirus (HCMV) results in activation of cellular interferon-responsive gene expression. We demonstrate here that infection of human fibroblasts with herpes simplex virus type 1 (HSV-1) in the absence of protein synthesis also induces the expression of interferon-responsive genes. Five genes tested (encoding ISG54, IFI56, ISG15, 9-27 and MxA) were activated by infection with HSV-1, although the degree of response varied between the individual genes. HSV-1 was a less efficient inducer than HCMV. The effect was a consequence of binding of the virus particle to the cell surface or of the presence of virion components within the infected cell. Induction was mediated by a pathway other than the mechanism through which interferon-α mediates its effects on cellular gene expression.

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2000-09-01
2020-01-20
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References

  1. Ace, C. I., McKee, T. A., Ryan, J. M., Cameron, J. M. & Preston, C. M. ( 1989; ). Construction and characterization of a herpes simplex virus type 1 mutant unable to transinduce immediate-early gene expression. Journal of Virology 63, 2260-2269.
    [Google Scholar]
  2. Boyle, K. A., Pietropaolo, R. L. & Compton, T. ( 1999; ). Engagement of the cellular receptor for glycoprotein B of human cytomegalovirus activates the interferon-response pathway. Molecular and Cellular Biology 19, 3607-3613.
    [Google Scholar]
  3. Chebath, J., Merlin, G., Benech, P. & Revel, M. ( 1983; ). Interferon-induced 56,000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA. Nucleic Acids Research 11, 1213-1226.[CrossRef]
    [Google Scholar]
  4. Darnell, J. D., Kerr, I. M. & Stark, G. R. ( 1994; ). Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science 264, 1415-1421.[CrossRef]
    [Google Scholar]
  5. Der, S. D., Zhou, A., Williams, B. R. G. & Silverman, R. H. ( 1998; ). Identification of genes differentially regulated by interferon α, β and γ using oligonucleotide arrays. Proceedings of the National Academy of Sciences, USA 95, 15623-15628.[CrossRef]
    [Google Scholar]
  6. Guo, J., Peters, K. L. & Sen, G. C. ( 2000; ). Induction of the human protein P56 by interferon, double-stranded RNA, or virus infection. Virology 267, 209-219.[CrossRef]
    [Google Scholar]
  7. Hobbs, W. E. & DeLuca, N. A. ( 1999; ). Perturbation of cell cycle progression and cellular gene expression as a function of herpes simplex virus ICP0. Journal of Virology 73, 8245-8255.
    [Google Scholar]
  8. Homer, E. G., Rinaldi, A., Nicholl, M. J. & Preston, C. M. ( 1999; ). Activation of herpesvirus gene expression by the human cytomegalovirus protein pp71. Journal of Virology 73, 8512-8518.
    [Google Scholar]
  9. Kemp, L. M., Bricknell, P. M., LaThangue, N. B. & Latchman, D. S. ( 1986a; ). Transcriptional induction of cellular gene expression during lytic infection with herpes simplex virus. Bioscience Reports 6, 945-951.[CrossRef]
    [Google Scholar]
  10. Kemp, L. M., Preston, C. M., Preston, V. G. & Latchman, D. S. ( 1986b; ). Cellular gene induction during herpes simplex virus infection can occur without viral protein synthesis. Nucleic Acids Research 23, 9261-9270.
    [Google Scholar]
  11. Leib, D. A., Harrison, T. E., Laslo, K. M., Machalek, M. A., Moorman, N. J. & Virgin, H. W. ( 1999; ). Interferons regulate the phenotype of wild-type and mutant herpes simplex viruses in vivo. Journal of Experimental Medicine 189, 663-672.[CrossRef]
    [Google Scholar]
  12. McKendry, R., John, J., Flavell, D., Muller, M., Kerr, I. M. & Stark, G. R. ( 1991; ). High-frequency mutagenesis of human cells and characterization of a mutant unresponsive to both α and γ interferons. Proceedings of the National Academy of Sciences, USA 88, 11455-11459.[CrossRef]
    [Google Scholar]
  13. Marié, I., Durbin, J. E. & Levy, D. E. ( 1998; ). Differential viral induction of distinct interferon-a genes by positive feedback through interferon regulatory factor-7. EMBO Journal 17, 6660-6669.[CrossRef]
    [Google Scholar]
  14. Navarro, L., Mowen, K., Rodems, S., Weaver, B., Reich, N., Spector, D. & David, M. ( 1998; ). Cytomegalovirus activates interferon immediate-early response gene expression and an interferon regulatory factor 3-containing interferon-stimulated response element-binding complex. Molecular and Cellular Biology 18, 3796-3802.
    [Google Scholar]
  15. Nicholl, M. J. & Preston, C. M. ( 1996; ). Inhibition of herpes simplex virus type 1 immediate-early gene expression by alpha interferon is not VP16 specific. Journal of Virology 70, 6336-6339.
    [Google Scholar]
  16. Notarianni, E. L. & Preston, C. M. ( 1982; ). Activation of cellular stress protein genes by herpes simplex virus temperature-sensitive mutants which overproduce immediate early polypeptides. Virology 123, 113-122.[CrossRef]
    [Google Scholar]
  17. Pellegrini, S., John, J., Shearer, M., Kerr, I. M. & Stark, G. R. ( 1989; ). Use of a selectable marker regulated by alpha interferon to obtain mutations in the signalling pathway. Molecular and Cellular Biology 9, 4605-4612.
    [Google Scholar]
  18. Preston, V. G. ( 1990; ). Herpes simplex virus activates expression of a cellular gene by specific binding to the cell surface. Virology 176, 474-482.[CrossRef]
    [Google Scholar]
  19. Preston, C. M., Mabbs, R. & Nicholl, M. J. ( 1997; ). Construction and characterization of herpes simplex virus type 1 mutants with conditional defects in immediate early gene expression. Virology 229, 228-239.[CrossRef]
    [Google Scholar]
  20. Preston, C. M., Rinaldi, A. & Nicholl, M. J. ( 1998; ). Herpes simplex virus type 1 immediate early gene expression is stimulated by inhibition of protein synthesis. Journal of General Virology 79, 117-124.
    [Google Scholar]
  21. Stark, G. R., Kerr, I. M., Williams, B. R. G., Silverman, R. H. & Schreiber, R. D. ( 1998; ). How cells respond to interferons. Annual Review of Biochemistry 67, 227-264.[CrossRef]
    [Google Scholar]
  22. Szilágyi, J. F. & Cunningham, C. ( 1991; ). Identification and characterization of a novel non-infectious herpes simplex virus-related particle. Journal of General Virology 72, 661-668.[CrossRef]
    [Google Scholar]
  23. Wathelet, M. G., Berr, P. M. & Huez, G. A. ( 1992; ). Regulation of gene expression by cytokines and virus in human cells lacking the type-I interferon locus. European Journal of Biochemistry 206, 901-910.[CrossRef]
    [Google Scholar]
  24. Wathelet, M. G., Lin, C. H., Parekh, B. S., Ronco, L. V., Howley, P. M. & Maniatis, T. ( 1998; ). Virus infection induces the assembly of coordinately activated transcription factors on the IFN-β enhancer in vivo. Molecular Cell 1, 507-518.[CrossRef]
    [Google Scholar]
  25. Zhu, H., Cong, J.-P. & Shenk, T. ( 1997; ). Use of differential display analysis to assess the effect of human cytomegalovirus infection on the accumulation of cellular RNAs: induction of interferon-responsive RNAs. Proceedings of the National Academy of Sciences, USA 94, 13985-13990.[CrossRef]
    [Google Scholar]
  26. Zhu, H., Cong, J.-P., Mamtora, G., Gingeras, T. & Shenk, T. ( 1998; ). Cellular gene expression altered by human cytomegalovirus: global monitoring with oligonucleotide arrays. Proceedings of the National Academy of Sciences, USA 95, 14470-14475.[CrossRef]
    [Google Scholar]
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