1887

Abstract

Previous studies have shown that infection of human fibroblasts with human cytomegalovirus (HCMV) results in activation of cellular interferon-responsive gene expression. We demonstrate here that infection of human fibroblasts with herpes simplex virus type 1 (HSV-1) in the absence of protein synthesis also induces the expression of interferon-responsive genes. Five genes tested (encoding ISG54, IFI56, ISG15, 9-27 and MxA) were activated by infection with HSV-1, although the degree of response varied between the individual genes. HSV-1 was a less efficient inducer than HCMV. The effect was a consequence of binding of the virus particle to the cell surface or of the presence of virion components within the infected cell. Induction was mediated by a pathway other than the mechanism through which interferon-α mediates its effects on cellular gene expression.

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2000-09-01
2020-04-07
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References

  1. Ace C. I., McKee T. A., Ryan J. M., Cameron J. M., Preston C. M.. 1989; Construction and characterization of a herpes simplex virus type 1 mutant unable to transinduce immediate-early gene expression. Journal of Virology63:2260–2269
    [Google Scholar]
  2. Boyle K. A., Pietropaolo R. L., Compton T.. 1999; Engagement of the cellular receptor for glycoprotein B of human cytomegalovirus activates the interferon-response pathway. Molecular and Cellular Biology19:3607–3613
    [Google Scholar]
  3. Chebath J., Merlin G., Benech P., Revel M.. 1983; Interferon-induced 56,000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA. Nucleic Acids Research11:1213–1226
    [Google Scholar]
  4. Darnell J. D., Kerr I. M., Stark G. R.. 1994; Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science264:1415–1421
    [Google Scholar]
  5. Der S. D., Zhou A., Williams B. R. G., Silverman R. H.. (1998; Identification of genes differentially regulated by interferon α, β and γ using oligonucleotide arrays. Proceedings of the National Academy of Sciences, USA95:15623–15628
    [Google Scholar]
  6. Guo J., Peters K. L., Sen G. C.. 2000; Induction of the human protein P56 by interferon, double-stranded RNA, or virus infection. Virology267:209–219
    [Google Scholar]
  7. Hobbs W. E., DeLuca N. A.. 1999; Perturbation of cell cycle progression and cellular gene expression as a function of herpes simplex virus ICP0. Journal of Virology73:8245–8255
    [Google Scholar]
  8. Homer E. G., Rinaldi A., Nicholl M. J., Preston C. M.. 1999; Activation of herpesvirus gene expression by the human cytomegalovirus protein pp71. Journal of Virology73:8512–8518
    [Google Scholar]
  9. Kemp L. M., Bricknell P. M., LaThangue N. B., Latchman D. S.. 1986a; Transcriptional induction of cellular gene expression during lytic infection with herpes simplex virus. Bioscience Reports6:945–951
    [Google Scholar]
  10. Kemp L. M., Preston C. M., Preston V. G., Latchman D. S.. 1986b; Cellular gene induction during herpes simplex virus infection can occur without viral protein synthesis. Nucleic Acids Research23:9261–9270
    [Google Scholar]
  11. Leib D. A., Harrison T. E., Laslo K. M., Machalek M. A., Moorman N. J., Virgin H. W.. (1999; Interferons regulate the phenotype of wild-type and mutant herpes simplex viruses in vivo. Journal of Experimental Medicine189:663–672
    [Google Scholar]
  12. McKendry R., John J., Flavell D., Muller M., Kerr I. M., Stark G. R.. 1991; High-frequency mutagenesis of human cells and characterization of a mutant unresponsive to both α and γ interferons. Proceedings of the National Academy of Sciences, USA88:11455–11459
    [Google Scholar]
  13. Marié I., Durbin J. E., Levy D. E.. 1998; Differential viral induction of distinct interferon-a genes by positive feedback through interferon regulatory factor-7. EMBO Journal17:6660–6669
    [Google Scholar]
  14. Navarro L., Mowen K., Rodems S., Weaver B., Reich N., Spector D., David M.. 1998; Cytomegalovirus activates interferon immediate-early response gene expression and an interferon regulatory factor 3-containing interferon-stimulated response element-binding complex. Molecular and Cellular Biology18:3796–3802
    [Google Scholar]
  15. Nicholl M. J., Preston C. M.. 1996; Inhibition of herpes simplex virus type 1 immediate-early gene expression by alpha interferon is not VP16 specific. Journal of Virology70:6336–6339
    [Google Scholar]
  16. Notarianni E. L., Preston C. M.. 1982; Activation of cellular stress protein genes by herpes simplex virus temperature-sensitive mutants which overproduce immediate early polypeptides. Virology123:113–122
    [Google Scholar]
  17. Pellegrini S., John J., Shearer M., Kerr I. M., Stark G. R.. 1989; Use of a selectable marker regulated by alpha interferon to obtain mutations in the signalling pathway. Molecular and Cellular Biology9:4605–4612
    [Google Scholar]
  18. Preston V. G.. 1990; Herpes simplex virus activates expression of a cellular gene by specific binding to the cell surface. Virology176:474–482
    [Google Scholar]
  19. Preston C. M., Mabbs R., Nicholl M. J.. 1997; Construction and characterization of herpes simplex virus type 1 mutants with conditional defects in immediate early gene expression. Virology229:228–239
    [Google Scholar]
  20. Preston C. M., Rinaldi A., Nicholl M. J.. 1998; Herpes simplex virus type 1 immediate early gene expression is stimulated by inhibition of protein synthesis. Journal of General Virology79:117–124
    [Google Scholar]
  21. Stark G. R., Kerr I. M., Williams B. R. G., Silverman R. H., Schreiber R. D.. 1998; How cells respond to interferons. Annual Review of Biochemistry67:227–264
    [Google Scholar]
  22. Szilágyi J. F., Cunningham C.. 1991; Identification and characterization of a novel non-infectious herpes simplex virus-related particle. Journal of General Virology72:661–668
    [Google Scholar]
  23. Wathelet M. G., Berr P. M., Huez G. A.. 1992; Regulation of gene expression by cytokines and virus in human cells lacking the type-I interferon locus. European Journal of Biochemistry206:901–910
    [Google Scholar]
  24. Wathelet M. G., Lin C. H., Parekh B. S., Ronco L. V., Howley P. M., Maniatis T.. 1998; Virus infection induces the assembly of coordinately activated transcription factors on the IFN-β enhancer in vivo. Molecular Cell1:507–518
    [Google Scholar]
  25. Zhu H., Cong J.-P., Shenk T.. 1997; Use of differential display analysis to assess the effect of human cytomegalovirus infection on the accumulation of cellular RNAs: induction of interferon-responsive RNAs. Proceedings of the National Academy of Sciences, USA94:13985–13990
    [Google Scholar]
  26. Zhu H., Cong J.-P., Mamtora G., Gingeras T., Shenk T.. 1998; Cellular gene expression altered by human cytomegalovirus: global monitoring with oligonucleotide arrays. Proceedings of the National Academy of Sciences, USA95:14470–14475
    [Google Scholar]
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