1887

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Volume 81, Issue 9

Processing of GB virus B non-structural proteins in cultured cells requires both NS3 protease and NS4A cofactor Free

Abstract

The identification of antivirals and vaccines against hepatitis C virus (HCV) infection is hampered by the lack of convenient animal models. The need to develop surrogate models has recently drawn attention to GB virus B (GBV-B), which produces hepatitis in small primates. In a previous study , it was shown that GBV-B NS3 protease shares substrate specificity with the HCV enzyme, known to be crucial for virus replication. In this report, GBV-B NS3 activity on GBV-B precursor proteins has been analysed in a cell-based system. It is shown that mature protein products are obtained that are compatible with the cleavage sites proposed on the basis of sequence homology with HCV and that GBV-B NS4A protein is required as a cofactor for optimal enzymatic activity. Experiments supported by a structural model mapped the region of NS4A that interacts with NS3 and showed that the GBV-B cofactor cannot be substituted for by its HCV analogue.

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© Microbiology Society
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2000-09-01
2024-04-24
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Processing of GB virus B non-structural proteins in cultured cells requires both NS3 protease and NS4A cofactor
J. Gen. Virol. 81, 2183 (2000); https://doi.org/10.1099/0022-1317-81-9-2183
/content/journal/jgv/10.1099/0022-1317-81-9-2183
/content/journal/jgv/10.1099/0022-1317-81-9-2183
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