1887

Abstract

Rift Valley fever virus (RVFV) is an arbovirus of the family, causing recurrent disease outbreaks in Africa. Natural vertebrate hosts include cattle and humans. Several mosquito species belonging to the and generaact as vectors of this phlebovirus. To test whether pathogen-derived resistance against RVFV could be induced by expressing genomic sequences in mosquito cells, as has been shown for La Crosse and dengue 2 viruses, we generated various recombinant Semliki Forest viruses expressing the S segment (or its genes) in the genomic or antigenomic sense. Expression of the N but not the NSs gene interfered with the production of RVFV in mosquito cells and this phenomenon was RNA- but not protein-dependent. These results raise questions on the molecular mechanisms involved in virus resistance.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-9-2161
2000-09-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/9/0812161a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-9-2161&mimeType=html&fmt=ahah

References

  1. Angell, S. M. & Baulcombe, D. C. ( 1997; ). Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA. EMBO Journal 16, 3675-3684.[CrossRef]
    [Google Scholar]
  2. Baulcombe, D. ( 1999; ). Viruses and gene silencing in plants. Archives of Virology Supplementum 15, 189-201.
    [Google Scholar]
  3. Beaty, B. J., Bishop, D. H., Gay, M. & Fuller, F. ( 1983; ). Interference between bunyaviruses in Aedes triseriatus mosquitoes. Virology 127, 83-90.[CrossRef]
    [Google Scholar]
  4. Cogoni, C. & Macino, G. ( 1999; ). Homology-dependent gene silencing in plants and fungi: a number of variations on the same theme. Current Opinion in Microbiology 2, 657-662.[CrossRef]
    [Google Scholar]
  5. Dalmay, T., Hamilton, A., Mueller, E. & Baulcombe, D. C. ( 2000; ). Potato virus X amplicons in Arabidopsis mediate genetic and epigenetic gene silencing. Plant Cell 12, 369-379.[CrossRef]
    [Google Scholar]
  6. Elliott, R. M. & Wilkie, M. L. ( 1986; ). Persistent infection of Aedes albopictus C6/36 cells by Bunyamwera virus. Virology 150, 21-32.[CrossRef]
    [Google Scholar]
  7. English, J. J., Mueller, E. & Baulcombe, D. C. ( 1996; ). Suppression of virus accumulation in transgenic plants exhibiting silencing of nuclear genes. Plant Cell 8, 179-188.[CrossRef]
    [Google Scholar]
  8. Fire, A. ( 1999; ). RNA-triggered gene silencing. Trends in Genetics 15, 358-363.[CrossRef]
    [Google Scholar]
  9. Gaines, P. J., Olson, K. E., Higgs, S., Powers, A. M., Beaty, B. J. & Blair, C. D. ( 1996; ). Pathogen-derived resistance to dengue type 2 virus in mosquito cells by expression of the premembrane coding region of the viral genome. Journal of Virology 70, 2132-2137.
    [Google Scholar]
  10. Giorgi, C. ( 1996; ). Molecular biology of phlebovirus. In The Bunyaviridae, pp. 105-128. Edited by R. M. Elliott. New York: Plenum Press.
  11. Gonzalez-Scarano, F. & Nathanson, N. ( 1996; ). Bunyaviridae. In Fields Virology, pp. 1473-1504. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott–Raven.
  12. Hunter, C. P. ( 2000; ). Gene silencing: shrinking the black box of RNAi. Current Biology 10, R137-R140.[CrossRef]
    [Google Scholar]
  13. Jan, F. J., Fagoaga, C., Pang, S. Z. & Gonsalves, D. ( 2000; ). A minimum length of N gene sequence in transgenic plants is required for RNA-mediated tospovirus resistance. Journal of General Virology 81, 235-242.
    [Google Scholar]
  14. Karpf, A. R., Lenches, E., Strauss, E. G., Strauss, J. H. & Brown, D. T. ( 1997; ). Superinfection exclusion of alphaviruses in three mosquito cell lines persistently infected with Sindbis virus. Journal of Virology 71, 7119-7123.
    [Google Scholar]
  15. Kohl, A., di Bartolo, V. & Bouloy, M. ( 1999; ). The Rift Valley fever virus nonstructural protein NSs is phosphorylated at serine residues located in casein kinase II consensus motifs in the carboxy-terminus. Virology 263, 517-525.[CrossRef]
    [Google Scholar]
  16. Liljeström, P. & Garoff, H. ( 1991; ). A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Biotechnology 9, 1356-1361.[CrossRef]
    [Google Scholar]
  17. Liljeström, P. & Garoff, H. (1995). Expression of proteins using Semliki Forest virus vectors. In Current Protocols in Molecular Biology, pp. 16.20.1–16.20.16. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Wiley.
  18. Meegan, J. M. & Bailey, C. J. ( 1989; ). Rift Valley fever. In The Arboviruses: Epidemiology and Ecology, pp. 51-76. Edited by T. P. Monath. Boca Raton, FL: CRC Press.
  19. Müller, R., Saluzzo, J. F., Lopez, N., Dreier, T., Turell, M., Smith, J. & Bouloy, M. ( 1995; ). Characterization of clone 13, a naturally attenuated avirulent isolate of Rift Valley fever virus, which is altered in the small segment. American Journal of Tropical Medicine and Hygiene 53, 405-411.
    [Google Scholar]
  20. Olson, K. E., Higgs, S., Gaines, P. J., Powers, A. M., Davis, B. S., Kamrud, K. I., Carlson, J. O., Blair, C. D. & Beaty, B. J. ( 1996; ). Genetically engineered resistance to dengue-2 virus transmission in mosquitoes. Science 272, 884-886.[CrossRef]
    [Google Scholar]
  21. Peters, C. J. & Linthicum, K. J. ( 1994; ). Rift Valley fever. In Handbook of Zoonoses, section B, Viral, pp. 125-138. Edited by G. W. Beron & J. H. Steele. Boca Raton, FL: CRC Press.
  22. Powers, A. M., Olson, K. E., Higgs, S., Carlson, J. O. & Beaty, B. J. ( 1994; ). Intracellular immunization of mosquito cells to La Crosse virus using a recombinant Sindbis virus vector. Virus Research 32, 57-67.[CrossRef]
    [Google Scholar]
  23. Powers, A. M., Kamrud, K. I., Olson, K. E., Higgs, S., Carlson, J. O. & Beaty, B. J. ( 1996; ). Molecularly engineered resistance to California serogroup virus replication in mosquito cells and mosquitoes. Proceedings of the National Academy of Sciences, USA 93, 4187-4191.[CrossRef]
    [Google Scholar]
  24. Schmaljohn, C. S. ( 1996; ). Bunyaviridae: the viruses and their replication. In Fields Virology, pp. 1447-1471. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott–Raven.
  25. Schmaljohn, C. & Blair, C. D. ( 1977; ). Persistent infection of cultured mammalian cells by Japanese encephalitis virus. Journal of Virology 24, 580-589.
    [Google Scholar]
  26. Sijen, T., Wellink, J., Hiriart, J. B. & Kammen, A. V. ( 1996; ). RNA-mediated virus resistance: role of repeated transgenes and delineation of targeted regions. Plant Cell 8, 2277-2294.[CrossRef]
    [Google Scholar]
  27. Stollar, V. & Shenk, T. E. ( 1973; ). Homologous viral interference in Aedes albopictus cultures chronically infected with Sindbis virus. Journal of Virology 11, 592-595.
    [Google Scholar]
  28. Turell, M. J., Saluzzo, J. F., Tammariello, R. F. & Smith, J. F. ( 1990; ). Generation and transmission of Rift Valley fever viral reassortants by the mosquito Culex pipiens. Journal of General Virology 71, 2307-2312.[CrossRef]
    [Google Scholar]
  29. Vialat, P., Billecocq, A., Kohl, A. & Bouloy, M. ( 2000; ). The S segment of Rift Valley fever phlebovirus (Bunyaviridae) carries determinants for attenuation and virulence in mice. Journal of Virology 74, 1538-1543.[CrossRef]
    [Google Scholar]
  30. Waterhouse, P. M., Graham, M. W. & Wang, M. B. ( 1998; ). Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proceedings of the National Academy of Sciences, USA 95, 13959-13964.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-9-2161
Loading
/content/journal/jgv/10.1099/0022-1317-81-9-2161
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error