A single chimeric transgene derived from two distinct viruses confers multi-virus resistance in transgenic plants through homology-dependent gene silencing

References

1. Baulcombe D. C. 1996; Mechanisms of pathogen-derived resistance to viruses in transgenic plants. Plant Cell 8:1833–1844
   [Google Scholar]
2. Baulcombe D. C., English J. J. 1996; Ectopic pairing of homologous DNA and post-transcriptional gene silencing in transgenic plants. Current Opinion in Biotechnology 7:173–180
   [Google Scholar]
3. Beachy R. N. 1997; Mechanisms and applications of pathogen-derived resistance in transgenic plants. Current Opinion in Biotechnology 8:215–220
   [Google Scholar]
4. Crété P., Vaucheret H. 1999; Expression and sequence requirements for nitrite reductase co-suppression. Plant Molecular Biology 41:105–114
   [Google Scholar]
5. Dawson W. D. 1996; Gene silencing and virus resistance: a common mechanism. Trends in Plant Science 1:107–108
   [Google Scholar]
6. de Haan P., Wagemakers L., Peters D., Goldbach R. 1990; The S RNA segment of tomato spotted wilt virus has an ambisense character. Journal of General Virology 71:1001–1007
   [Google Scholar]
7. de Haan P., Kormelink R., Resende R. de O., van Poelwijk F., Peters D., Goldbach R. 1991; Tomato spotted wilt virus L RNA encodes a putative RNA polymerase. Journal of General Virology 72:2207–2216
   [Google Scholar]
8. Dehio C., Schell J. 1994; Identification of plant genetic loci involved in a posttranscriptional mechanism for meiotically reversible transgene silencing. Proceedings of the National Academy of Sciences, USA 91:5538–5542
   [Google Scholar]
9. Dougherty W. G., Carrington J. C. 1988; Expression and function of potyviral gene products. Annual Review of Phytopathology 26:123–143
   [Google Scholar]
10. Dougherty W. G., Parks T. D. 1995; Transgenes and gene suppression: telling us something new?Current Opinion in. Cell Biology 7:399–405
    [Google Scholar]
11. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
12. Fuchs M., Gonsalves D. 1995; Resistance of transgenic hybrid squash ZW-20 expressing the coat protein genes of zucchini yellow mosaic virus and watermelon mosaic virus 2 to mixed infections by both potyviruses. Bio/Technology 13:1466–1473
    [Google Scholar]
13. Fuchs M., McFerson J. R., Tricoli D. M., McMaster J. R., Deng R. Z., Boeshore M. L., Reynolds J. F., Russell P. F., Quemada H. D., Gonsalves D. 1997; Cantaloupe line CZW-30 containing coat protein genes of cucumber mosaic virus, zucchini yellow mosaic virus, and watermelon mosaic virus-2 is resistant to these three viruses in the field. Molecular Breeding 3:279–290
    [Google Scholar]
14. Fuchs M., Tricoli D. M., Carney K. J., Schesser M., McFerson J. R., Gonsalves D. 1998; Comparative virus resistance and fruit yield of transgenic squash with single and multiple coat protein genes. Plant Disease 82:1350–1356
    [Google Scholar]
15. German T. L., Ullman D. E., Moyer J. W. 1992; Tospoviruses (i): diagnosis, molecular biology, phylogeny, and vector relationships. Annual Review of Phytopathology 30:315–348
    [Google Scholar]
16. Green S. K., Deng T. C. 1985; Turnip mosaic virus strains in cruciferous hosts in Taiwan. Plant Disease 69:28–31
    [Google Scholar]
17. Horsch R. B., Fry J. E., Hoffmann N. L., Eichholtz D., Rogers S. G., Fraley R. T. 1985; A simple and general method for transferring genes into plants. Science 227:1229–1231
    [Google Scholar]
18. Jacobs J. J. M. R., Sanders M., Bots M., Andriessen M., van Eldik G. J., Litière K., Van Montagu M., Cornelissen M. 1999; Sequences throughout the basic β-1,3-glucanase mRNA coding region are targets for homology dependent post-transcriptional gene silencing. Plant Journal 20:143–152
    [Google Scholar]
19. Jan F.-J., Pang S.-Z., Fagoaga F., Gonsalves D. 1999; Turnip mosaic potyvirus resistance in Nicotiana benthamiana derived by post-transcriptional gene silencing. Transgenic Research 8:203–213
    [Google Scholar]
20. Jan F.-J., Fagoaga C., Pang S.-Z., Gonsalves D. 2000; A minimum length of N gene sequence in transgenic plants is required for RNA-mediated tospovirus resistance. Journal of General Virology 81:235–242
    [Google Scholar]
21. Kaniewski W., Lawson C., Sammons B., Haley L., Hart J., Delannay X., Tumer N. E. 1990; Field resistance of transgenic Russet Burbank potato to effects of infection by potato virus X and potato virus Y. Bio/Technology 8:750–754
    [Google Scholar]
22. Kaniewski W., Ilardi V., Tomassoli L., Mitsky T., Layton J., Barba M. 1999; Extreme resistance to cucumber mosaic virus (CMV) in transgenic tomato expressing one or two viral coat proteins. Molecular Breeding 5:111–119
    [Google Scholar]
23. Kormelink R., de Haan P., Peters D., Goldbach R. 1992a; Viral RNA synthesis in tomato spotted wilt virus-infected Nicotiana rustica plants. Journal of General Virology 73:687–693
    [Google Scholar]
24. Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R. 1992b; The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments. Journal of General Virology 73:2795–2804
    [Google Scholar]
25. Kormelink R., Storms M., van Lent J., Peters D., Goldbach R. 1994; Expression and subcellular location of the NS_M protein of tomato spotted wilt virus (TSWV), a putative viral movement protein. Virology 200:56–65
    [Google Scholar]
26. Lawson C., Kaniewski W., Haley L., Rozman R., Newell C., Sanders P., Tumer N. E. 1990; Engineering resistance to mixed virus infection in a commercial potato cultivar: resistance to potato virus X and potato virus Y in transgenic Russet Burbank. Bio/Technology 8:127–134
    [Google Scholar]
27. Ling K. S., Provvidenti R., Gonsalves D. 1995; Detection of turnip mosaic virus isolates using an antiserum to coat protein breakdown products. Plant Disease 79:809–812
    [Google Scholar]
28. Maiss E., Ivanova L., Breyel E., Adam G. 1991; Cloning and sequencing of the S RNA from a Bulgarian isolate of tomato spotted wilt virus. Journal of General Virology 72:461–464
    [Google Scholar]
29. Marano M. R., Baulcombe D. 1998; Pathogen-derived resistance targeted against the negative-strand RNA of tobacco mosaic virus: RNA strand-specific gene silencing?. Plant Journal 13:537–546
    [Google Scholar]
30. Napoli C., Lemieux C., Jorgensen R. 1990; Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2:279–290
    [Google Scholar]
31. Pang S.-Z., Nagpala P., Wang M., Slightom J. L., Gonsalves D. 1992; Resistance to heterologous isolates of tomato spotted wilt virus in transgenic tobacco expressing its nucleocapsid protein gene. Phytopathology 82:1223–1229
    [Google Scholar]
32. Pang S.-Z., Jan F.-J., Carney K., Stout J., Tricoli D. M., Quemada H. D., Gonsalves D. 1996; Post-transcriptional transgene silencing and consequent tospovirus resistance in transgenic lettuce are affected by transgene dosage and plant development. Plant Journal 9:899–909
    [Google Scholar]
33. Pang S.-Z., Jan F.-J., Gonsalves D. 1997; Nontarget DNA sequences reduce the transgene length necessary for RNA-mediated tospovirus resistance in transgenic plants. Proceedings of the National Academy of Sciences, USA 94:8261–8266
    [Google Scholar]
34. Prins M., Goldbach R. 1996; RNA-mediated virus resistance in transgenic plants. Archives of Virology 141:2259–2276
    [Google Scholar]
35. Prins M., de Haan P., Luyten R., van Veller M., van Grinsven M. Q. J. M., Goldbach R. 1995; Broad resistance to tospoviruses in transgenic tobacco plants expressing three tospoviral nucleoprotein gene sequences. Molecular Plant–Microbe Interactions 8:85–91
    [Google Scholar]
36. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
37. Seymour G. B., Fray R. G., Hill P., Tucker G. A. 1993; Down-regulation of two non-homologous tomato genes with a single chimaeric sense gene construct. Plant Molecular Biology 23:1–9
    [Google Scholar]
38. Shukla D. D., Ward C. W., Brunt A. A. 1994 The Potyviridae Wallingford, UK: CAB International;
    [Google Scholar]
39. Stam M., de Bruin R., Kenter S., van der Hoorn R. A. L., van Blokland R., Mol J. N. M., Kooter J. M. 1997; Post-transcriptional silencing of chalcone synthase in Petunia by inverted transgene repeats. Plant Journal 12:63–82
    [Google Scholar]
40. Tomlinson J. A. 1987; Epidemiology and control of virus diseases of vegetables. Annals of Applied Biology 110:661–681
    [Google Scholar]
41. Tricoli D. M., Carney K. J., Russell P. F., McMaster J. R., Groff D. W., Hadden K. C., Himmel P. T., Hubbard J. P., Boeshore M. L., Quemada H. D. 1995; Field evaluation of transgenic squash containing single or multiple virus coat protein gene constructs for resistance to cucumber mosaic virus, watermelon mosaic virus 2, and zucchini yellow mosaic virus. Bio/Technology 13:1458–1465
    [Google Scholar]
42. Van Blokland R., van der Geest N., Mol J. N. M., Kooter J. M. 1994; Transgene-mediated suppression of chalcone synthase expression in Petunia hybrida results from an increase in RNA turnover. Plant Journal 6:861–877
    [Google Scholar]
43. van den Boogaart T., Lomonossoff G. P., Davies J. W. 1998; Can we explain RNA-mediated virus resistance by homology-dependent gene silencing?Molecular Plant–Microbe Interactions. 11717–723
44. Wang M., Gonsalves D. 1990; ELISA detection of various tomato spotted wilt virus isolates using specific antisera to structural proteins of the virus. Plant Disease 74:154–158
    [Google Scholar]