1887

Abstract

Channel catfish virus (CCV), a member of the herpesvirus family, causes a severe haemorrhagic disease in juvenile channel catfish. In this report, we confirm that CCV gene expression is temporally regulated into immediate-early (IE), early and late phases, similar to that of other herpesviruses. The transcriptional regulation of the 14 genes within the direct repeat region of the CCV genome was determined by Northern hybridization analysis of RNA isolated from infected cells in the presence or absence of metabolic inhibitors. Two CCV genes within the direct repeat, ORFs 1 and 3, expressed IE transcripts. Early RNAs were encoded by ORFs 2–9 and 11–14. ORFs 4, 7 and 10–13 expressed late transcripts after the onset of viral DNA replication. A time-course study conducted without metabolic inhibitors confirmed that CCV direct repeat transcription is temporally regulated. The characterization of CCV transcription during cytolytic infection will provide a foundation for the analysis of CCV gene expression in tissues of acutely and latently infected catfish.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-8-2005
2000-08-01
2020-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/8/0812005a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-8-2005&mimeType=html&fmt=ahah

References

  1. Baxi M. K., Efstathiou S., Lawrence G., Whalley J. M., Slater J. D., Field H. J.. 1995; The detection of latency-associated transcripts of equine herpesvirus 1 in ganglionic neurons. Journal of General Virology76:3113–3118
    [Google Scholar]
  2. Buck C.. 1990; Channel catfish virus – a review. ATCC Quarterly Newsletter3:1–2
    [Google Scholar]
  3. Chomczynski P., Sacchi N.. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction. Analytical Biochemistry162:156–159
    [Google Scholar]
  4. Davison A. J.. 1992; Channel catfish virus: a new type of herpesvirus. Virology186:9–14
    [Google Scholar]
  5. Davison A. J.. 1998; The genome of salmonid herpesvirus 1. Journal of Virology72:1974–1982
    [Google Scholar]
  6. Dixon R. A. F., Farber F. E.. 1980; Channel catfish virus: physiochemical properties of the viral genome and identification of viral polypeptides. Virology103:267–278
    [Google Scholar]
  7. Everett R. D.. 1987; The regulation of transcription of viral and cellular genes by herpesvirus immediate-early gene products (review). Anticancer Research7:589–604
    [Google Scholar]
  8. Gray W. L., Williams R. J., Griffin B.. 1998; Detection of channel catfish virus DNA in acutely infected channel catfish, Ictalurus punctatus (Rafinesque), using the polymerase chain reaction. Journal of Fish Diseases22:111–116
    [Google Scholar]
  9. Gray W. L., Williams R. J., Jordan R. L., Griffin B.. 1999; Detection of channel catfish virus DNA in latently infected catfish. Journal of General Virology80:1817–1822
    [Google Scholar]
  10. Hanson L. A., Thune R. L.. 1993; Characterization of thymidine kinase encoded by channel catfish virus. Journal of Aquatic Animal Health5:199–204
    [Google Scholar]
  11. Huang S. M., Hanson L. A.. 1998; Temporal gene regulation of the channel catfish virus (ictalurid herpesvirus 1). Journal of Virology72:1910–1917
    [Google Scholar]
  12. Mitchell W. J., Lirette R. P., Fraser N. W.. 1990; Mapping of low abundance latency-associated RNA in the trigeminal ganglia of mice latently infected with herpes simplex virus type 1. Journal of General Virology71:125–132
    [Google Scholar]
  13. Pearson W. R., Lipman D. J.. 1988; Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences USA:85:2444–2448
    [Google Scholar]
  14. Plumb J. A.. 1977; Channel catfish virus disease. U.S. Department of Interior pamphlet
    [Google Scholar]
  15. Priola S. A., Stevens J. G.. 1991; The 5′ and 3′ limits of transcription in the pseudorabies virus latency associated transcription unit. Virology182:852–856
    [Google Scholar]
  16. Silverstein P. S., Bird R. C., Van Santen V. L., Nusbaum K. E.. 1995; Immediate-early transcription from the channel catfish virus genome: characterization of two immediate-early transcripts. Journal of Virology69:3161–3166
    [Google Scholar]
  17. Silverstein P. S., Van Santen V. L., Nusbaum K. E., Bird R. C.. 1998; Expression kinetics and mapping of the thymidine kinase transcript and an immediate-early transcript from channel catfish virus. Journal of Virology72:3900–3906
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-8-2005
Loading
/content/journal/jgv/10.1099/0022-1317-81-8-2005
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error