1887

Abstract

M nucleopolyhedrovirus (AcMNPV) is the prototypical member of the genus of the , a family of large, double-stranded DNA viruses that are highly diverse. Nucleocapsid morphogenesis of AcMNPV and others in the genus takes place within the nuclei of infected host cells. Previously, we showed that filamentous actin (F-actin) is essential for this process to occur in AcMNPV-infected cells, an unprecedented finding for a DNA virus that replicates within the nucleus. Because of the fundamental importance of this requirement to our understanding of virus–host interactions, and because of the diversity of viruses included within the genus, we were compelled to determine whether the replication of other nucleopolyhedroviruses was also F-actin dependent. We report here that progeny virus production of six other lepidopteran nucleopolyhedroviruses, representing both phylogenetic groups I and II within the genus, is also F-actin dependent. The six viruses studied ( MNPV, NPV, MNPV, MNPV, MNPV and SNPV) were unable to produce progeny in the presence of either cytochalasin D or latrunculin A, two actin-binding agents that interfere with F-actin-dependent processes but differ in their modes of action. F-actin-dependent progeny morphogenesis, therefore, appears to be a characteristic common among viruses in this genus that have lepidopteran hosts.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-7-1881
2000-07-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/7/0811881a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-7-1881&mimeType=html&fmt=ahah

References

  1. Ahrens, C. H., Russell, R. L. Q., Funk, C. J., Evans, J. T., Harwood, S. H. & Rohrmann, G. F. ( 1997; ). The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome. Virology 229, 381-399.[CrossRef]
    [Google Scholar]
  2. Brown, S. S. & Spudich, J. A. ( 1981; ). Mechanism of action of cytochalasin: evidence that it binds to actin filament ends. Journal of Cell Biology 88, 487-491.[CrossRef]
    [Google Scholar]
  3. Bulach, D. M., Kumar, C. A., Zaia, A., Liang, B. & Tribe, D. E. ( 1999; ). Group II nucleopolyhedrovirus subgroups revealed by phylogenetic analysis of polyhedrin and DNA polymerase gene sequences. Journal of Invertebrate Pathology 73, 59-73.[CrossRef]
    [Google Scholar]
  4. Charlton, C. A. & Volkman, L. E. ( 1991; ). Sequential rearrangement and nuclear polymerization of actin in baculovirus-infected Spodoptera frugiperda cells. Journal of Virology 65, 1219-1227.
    [Google Scholar]
  5. Coué, M., Brenner, S. L., Spector, I. & Korn, E. D. ( 1987; ). Inhibition of actin polymerization by latrunculin A. FEBS Letters 213, 316-318.[CrossRef]
    [Google Scholar]
  6. Cudmore, S., Reckmann, I. & Way, M. ( 1997; ). Viral manipulations of the actin cytoskeleton. Trends in Microbiology 5, 142-148.[CrossRef]
    [Google Scholar]
  7. Engelhard, E. K., Kam-Morgan, L. N. W., Washburn, J. O. & Volkman, L. E. ( 1994; ). The insect tracheal system: a conduit for the systemic spread of Autographa californica M nuclear polyhedrosis virus. Proceedings of the National Academy of Sciences, USA 91, 3224-3227.[CrossRef]
    [Google Scholar]
  8. Gonsior, S. M., Platz, S., Buchmeier, S., Scheer, U. & Jockusch, B. M. ( 1999; ). Conformational difference between nuclear and cytoplasmic actin as detected by monoclonal antibody. Journal of Cell Science 112, 797-809.
    [Google Scholar]
  9. Hess, R. T., Goldsmith, P. A. & Volkman, L. E. ( 1989; ). Effect of cytochalasin D on cell morphology and AcMNPV replication in a Spodoptera frugiperda cell line. Journal of Invertebrate Pathology 53, 169-182.[CrossRef]
    [Google Scholar]
  10. Hom, L. G. & Volkman, L. E. ( 1998; ). Preventing proteolytic artifacts in the baculovirus expression system. BioTechniques 24, 18-19.
    [Google Scholar]
  11. Hu, Z. (1998). Characterization of the Buzura suppressaria single-nucleocapsid nucleopolyhedrovirus genome: a (phylo)genetic study. Dissertation, Wageningen University, Wageningen, The Netherlands.
  12. Keddie, B. A., Aponte, G. W. & Volkman, L. E. ( 1989; ). The pathway of infection of Autographa californica nuclear polyhedrosis virus in an insect host. Science 243, 1728-1730.[CrossRef]
    [Google Scholar]
  13. Kuzio, J., Pearson, M. N., Harwood, S. H., Funk, C. J., Evans, J. T., Slavicek, J. M. & Rohrman, G. F. ( 1999; ). Sequence and analysis of the genome of a baculovirus pathogenic for Lymantria dispar. Virology 253, 17-34.[CrossRef]
    [Google Scholar]
  14. Lanier, L. M. & Volkman, L. E. ( 1998; ). Actin binding and nucleation by Autographa californica M nucleopolyhedrovirus. Virology 243, 167-177.[CrossRef]
    [Google Scholar]
  15. Lessard, J. L. ( 1988; ). Two monoclonal antibodies to actin: one muscle selective and one generally reactive. Cell Motility and Cytoskeleton 10, 349-362.[CrossRef]
    [Google Scholar]
  16. Lynn, D. E. & Shapiro, M. ( 1998; ). New cell lines from Heliothis virescens: characterization and susceptibility to baculoviruses. Journal of Invertebrate Pathology 72, 276-280.[CrossRef]
    [Google Scholar]
  17. McIntosh, A. H., Andrews, P. A. & Ignoffo, C. M. ( 1981; ). Establishment of two continuous cell lines of Heliothis virescens (F.) (Lepidoptera: Noctuidae). In Vitro 17, 649-650.[CrossRef]
    [Google Scholar]
  18. Maeda, S. ( 1989; ). Gene transfer vectors of a baculovirus, Bombyx mori nuclear polyhedrosis virus, and their use for expression of foreign genes in insect cells. In Invertebrate Cell System Applications, pp. 167-181. Edited by J. Mitsuhashi. Boca Raton, FL: CRC Press.
  19. Miller, L. K. (1997). The Baculoviruses. New York: Plenum.
  20. Ohkawa, T. & Volkman, L. E. ( 1999; ). Nuclear F-actin is required for AcMNPV nucleocapsid morphogenesis. Virology 264, 1-4.[CrossRef]
    [Google Scholar]
  21. Russell, R. L. Q., Funk, C. J. & Rohrmann, G. F. ( 1997; ). Association of a baculovirus-encoded protein with the capsid basal region. Virology 227, 142-152.[CrossRef]
    [Google Scholar]
  22. Slavicek, J. S., Mercer, M. J., Kelly, M. E. & Hayes-Plazolles, N. ( 1996; ). Isolation of a baculovirus variant that exhibits enhanced polyhedra production stability during serial passage in cell culture. Journal of Invertebrate Pathology 67, 153-160.[CrossRef]
    [Google Scholar]
  23. Spector, I., Shochet, N. R., Blasberger, D. & Kashman, Y. ( 1989; ). Latrunculins–novel marine macrolides that disrupt microfilament organization and affect cell growth. I. Comparison with cytochlasin D. Cell Motility and the Cytoskeleton 13, 127-144.[CrossRef]
    [Google Scholar]
  24. Talhouk, S. N. & Volkman, L. E. ( 1991; ). Autographa californica M nuclear polyhedrosis virus and cytochalasin D: antagonists in the regulation of protein synthesis. Virology 182, 626-634.[CrossRef]
    [Google Scholar]
  25. Volkman, L. E. ( 1983; ). Occluded and budded Autographa californica nuclear polyhedrosis virus: immunological relatedness of structural proteins. Journal of Virology 46, 221-229.
    [Google Scholar]
  26. Volkman, L. E. ( 1988; ). Autographa californica MNPV nucleocapsid assembly: inhibition by cytochalasin D. Virology 163, 547-553.[CrossRef]
    [Google Scholar]
  27. Volkman, L. E. & Goldsmith, P. A. ( 1982; ). Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro. Applied and Environmental Microbiology 44, 227-233.
    [Google Scholar]
  28. Volkman, L. E., Goldsmith, P. A. & Hess, R. T. ( 1987; ). Evidence for microfilament involvement in budded Autographa californica nuclear polyhedrovirus virus production. Virology 156, 32-39.[CrossRef]
    [Google Scholar]
  29. Zanotto, P. M. A., Kessing, B. D. & Maruniak, J. E. ( 1993; ). Phylogenetic interrelationships among baculoviruses: evolutionary rates and host associations. Journal of Invertebrate Pathology 62, 147-164.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-7-1881
Loading
/content/journal/jgv/10.1099/0022-1317-81-7-1881
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error