1887

Abstract

Two types of porcine circovirus (PCV) have been isolated and are referred to as PCV1 and PCV2. PCV1 represents an apathogenic virus, whereas PCV2 is associated with post-weaning multisystemic wasting syndrome. The two PCVs are related, since they display about 70% identity based on nucleotide sequences. In order to discriminate between common and type-specific antigens, an immunocytological approach was used following transfections with cloned circovirus DNAs, as well as recombinant proteins expressed by either baculovirus or plasmid vectors. The ORF1-encoded proteins in the two viruses were shown to be antigenically related, whereas the ORF2 proteins were recognized differentially by polyclonal anti-PCV2 antibodies. Furthermore, PEPSCAN analysis performed on overlapping fragments of the genes encoding part of ORF1 and the entire ORF2 and ORF3 led to the identification of five dominant immunoreactive areas, one located on ORF1 and four on ORF2. However, only some ORF2 peptides proved to be immunorelevant epitopes for virus type discrimination. The potential use of ORF2-derived antigens as diagnostic tools is demonstrated.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-7-1815
2000-07-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/7/0811815a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-7-1815&mimeType=html&fmt=ahah

References

  1. Allan, G. M., McNeilly, F., Cassidy, J. P., Reilly, G. A., Adair, B., Ellis, W. A. & McNulty, M. S. ( 1995; ). Pathogenesis of porcine circovirus; experimental infections of colostrum deprived piglets and examination of pig foetal material. Veterinary Microbiology 44, 49-64.[CrossRef]
    [Google Scholar]
  2. Allan, G., Meehan, B., Todd, D., Kennedy, S., McNeilly, F., Ellis, J., Clark, E. G., Harding, J., Espuna, E., Botner, A. & Charreyre, C. ( 1998a; ). Novel porcine circoviruses from pigs with wasting disease syndromes. Veterinary Record 142, 467-468.
    [Google Scholar]
  3. Allan, G. M., McNeilly, F., Kennedy, S., Daft, B., Clark, E. G., Ellis, J. A., Haines, D. M., Meehan, B. M. & Adair, B. M. ( 1998b; ). Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe. Journal of Veterinary Diagnostic Investigation 10, 3-10.[CrossRef]
    [Google Scholar]
  4. Allan, G. M., Kennedy, S., McNeilly, F., Foster, J. C., Ellis, J. A., Krakowka, S. J., Meehan, B. M. & Adair, B. M. ( 1999a; ). Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. Journal of Comparative Pathology 121, 1-11.[CrossRef]
    [Google Scholar]
  5. Allan, G. M., McNeilly, F., Meehan, B. M., Kennedy, S., Mackie, D. P., Ellis, J. A., Clark, E. G., Espuna, E., Saubi, N., Riera, P., Botner, A. & Charreyre, C. E. ( 1999b; ). Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Veterinary Microbiology 66, 115-123.[CrossRef]
    [Google Scholar]
  6. Balasch, M., Segalés, J., Rosell, C., Domingo, M., Mankertz, A., Urniza, A. & Plana-Durán, J. ( 1999; ). Experimental inoculation of conventional pigs with tissue homogenates from pigs with post-weaning multisystemic wasting syndrome. Journal of Comparative Pathology 121, 139-148.[CrossRef]
    [Google Scholar]
  7. Bassami, M. R., Berryman, D., Wilcox, G. E. & Raidal, S. R. ( 1998; ). Psittacine beak and feather disease virus nucleotide sequence analysis and its relationship to porcine circovirus, plant circoviruses, and chicken anaemia virus. Virology 249, 453-459.[CrossRef]
    [Google Scholar]
  8. Dulac, G. C. & Afshar, A. ( 1989; ). Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Canadian Journal of Veterinary Research 53, 431-433.
    [Google Scholar]
  9. Edwards, S. & Sands, J. J. ( 1994; ). Evidence of circovirus infection in British pigs. Veterinary Record 134, 680-681.[CrossRef]
    [Google Scholar]
  10. Ellis, J., Hassard, L., Clark, E., Harding, J., Allan, G., Willson, P., Strokappe, J., Martin, K., McNeilly, F., Meehan, B., Todd, D. & Haines, D. ( 1998; ). Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Canadian Veterinary Journal 39, 44-51.
    [Google Scholar]
  11. Ellis, J., Krakowka, S., Lairmore, M., Haines, D., Bratanich, A., Clark, E., Allan, G., Konoby, C., Hassard, L., Meehan, B., Martin, K., Harding, J., Kennedy, S. & McNeilly, F. ( 1999; ). Reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. Journal of Veterinary Diagnostic Investigation 11, 3-14.[CrossRef]
    [Google Scholar]
  12. Hines, R. K. & Lukert, P. D. ( 1995; ). Porcine circovirus: a serological survey of swine in the United States. Swine Health and Production 3, 71-73.
    [Google Scholar]
  13. Kennedy, S., Allan, G., McNeilly, F., Adair, B. M., Hughes, A. & Spillane, P. ( 1998; ). Porcine circovirus infection in Northern Ireland. Veterinary Record 142, 495-496.
    [Google Scholar]
  14. Kiupel, M., Stevenson, G. W., Mittal, S. K., Clark, E. G. & Haines, D. M. ( 1998; ). Circovirus-like viral associated disease in weaned pigs in Indiana. Veterinary Pathology 35, 303-307.[CrossRef]
    [Google Scholar]
  15. Le Cann, P., Albina, E., Madec, F., Cariolet, R. & Jestin, A. ( 1997; ). Piglet wasting disease. Veterinary Record 141, 660.
    [Google Scholar]
  16. Lukert, P., de Boer, G. F., Dale, J. L., Keese, P., McNulty, M. S., Randles, J. W. & Tischer, I. ( 1995; ). Family Circoviridae. In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses, pp. 166-168. Edited by F. A. Murphy, C. M. Fauquet, D. H. L. Bishop, S. A. Ghabrial, A. W. Jarvis, G. P. Martelli, M. A. Mayo & M. D. Summers. Vienna & New York: Springer-Verlag.
  17. Madec, F., Eveno, E., Morvan, P., Hamon, L., Blanchard, P., Cariolet, R., Amenna, N., Morvan, H., Truong, C., Mahé, D., Albina, E. & Jestin, A. (2000). Post-weaning multisystemic wasting syndrome (PMWS) in pigs in France: clinical observations from follow-up studies on affected farms. Livestock Production Science (in press).
  18. Mankertz, A., Mankertz, J., Wolf, K. & Buhk, H.-J. ( 1998a; ). Identification of a protein essential for replication of porcine circovirus. Journal of General Virology 79, 381-384.
    [Google Scholar]
  19. Mankertz, J., Buhk, H.-J., Blaess, G. & Mankertz, A. ( 1998b; ). Transcription analysis of porcine circovirus (PCV). Virus Genes 16, 267-276.[CrossRef]
    [Google Scholar]
  20. Mankertz, A., Domingo, M., Folch, J. M., Le Cann, P., Jestin, A., Segalés, J., Chmielewicz, B., Plana-Durán, J. & Soike, D. ( 2000; ). Characterization of PCV-2 isolates from Spain, Germany and France. Virus Research 66, 65-77.[CrossRef]
    [Google Scholar]
  21. Meehan, B. M., Creelan, J. L., McNulty, M. S. & Todd, D. ( 1997; ). Sequence of porcine circovirus DNA: affinities with plant circoviruses. Journal of General Virology 78, 221-227.
    [Google Scholar]
  22. Meehan, B. M., McNeilly, F., Todd, D., Kennedy, S., Jewhurst, V. A., Ellis, J. A., Hassard, L. E., Clark, E. G., Haines, D. M. & Allan, G. M. ( 1998; ). Characterization of novel circovirus DNAs associated with wasting syndromes in pigs. Journal of General Virology 79, 2171-2179.
    [Google Scholar]
  23. Morozov, I., Sirinarumitr, T., Sorden, S. D., Halbur, P. G., Morgan, M. K., Yoon, K. J. & Paul, P. S. ( 1998; ). Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. Journal of Clinical Microbiology 36, 2535-2541.
    [Google Scholar]
  24. Nayar, G. P., Hamel, A. & Lin, L. ( 1997; ). Detection and characterization of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. Canadian Veterinary Journal 38, 385-386.
    [Google Scholar]
  25. Niagro, F. D., Forsthoefel, A. N., Lawther, R. P., Kamalanathan, L., Ritchie, B. W., Latimer, K. S. & Lukert, P. D. ( 1998; ). Beak and feather disease virus and porcine circovirus genomes: intermediates between the geminiviruses and plant circoviruses. Archives of Virology 143, 1723-1744.[CrossRef]
    [Google Scholar]
  26. Pringle, C. R. ( 1999; ). Virus taxonomy at the XIth International Congress of Virology, Sydney, Australia, 1999. Archives of Virology 144, 2065-2070.[CrossRef]
    [Google Scholar]
  27. Segalés, J., Sitjar, M., Domingo, M., Dee, S., Del Pozo, M., Noval, R., Sacristan, C., De las Heras, A., Ferro, A. & Latimer, K. S. ( 1997; ). First report of post-weaning multisystemic wasting syndrome in pigs in Spain. Veterinary Record 141, 600-601.
    [Google Scholar]
  28. Tischer, I., Gelderblom, H., Vettermann, W. & Koch, M. A. ( 1982; ). A very small porcine virus with circular single-stranded DNA. Nature 295, 64-66.[CrossRef]
    [Google Scholar]
  29. Tischer, I., Mields, W., Wolff, D., Vagt, M. & Griem, W. ( 1986; ). Studies on epidemiology and pathogenicity of porcine circovirus. Archives of Virology 91, 271-276.[CrossRef]
    [Google Scholar]
  30. Tischer, I., Peters, D., Rasch, R. & Pociuli, S. ( 1987; ). Replication of porcine circovirus: induction by glucosamine and cell cycle dependence. Archives of Virology 96, 39-57.[CrossRef]
    [Google Scholar]
  31. Tischer, I., Bode, L., Peters, D., Pociuli, S. & Germann, B. ( 1995; ). Distribution of antibodies to porcine circovirus in swine populations of different breeding farms. Archives of Virology 140, 737-743.[CrossRef]
    [Google Scholar]
  32. Todd, D., Creelan, J. L., Mackie, D. P., Rixon, F. & McNulty, M. S. ( 1990; ). Purification and biochemical characterization of chicken anaemia agent. Journal of General Virology 71, 819-823.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-7-1815
Loading
/content/journal/jgv/10.1099/0022-1317-81-7-1815
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error