1887

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Volume 81, Issue 7

Dengue virus type 1 DNA vaccine induces protective immune responses in rhesus macaques Free

Abstract

A candidate DNA vaccine expressing dengue virus type 1 pre-membrane and envelope proteins was used to immunize rhesus macaques. Monkeys were immunized intramuscularly (i.m.) or intradermally (i.d.) by three or four 1 mg doses of vaccine, respectively. Monkeys that were inoculated i.m. seroconverted more quickly and had higher antibody levels than those that were inoculated i.d. The sera exhibited virus-neutralizing activity, which declined over time. Four of the eight i.m.-inoculated monkeys were protected completely from developing viraemia when challenged 4 months after the last dose with homologous dengue virus. The other four monkeys had reduced viraemia compared with the control immunized monkeys. The i.d.-inoculated monkeys showed no reduction in viraemia when challenged with the virus. All vaccinated monkeys showed an anamnestic antibody response, indicating that they had established immunological memory. Vaccine-induced antibody had an avidity index similar to that of antibody induced by virus infection; however, no clear correlation was apparent between antibody avidity and virus neutralization titres.

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2000-07-01
2024-04-25
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Dengue virus type 1 DNA vaccine induces protective immune responses in rhesus macaques
J. Gen. Virol. 81, 1659 (2000); https://doi.org/10.1099/0022-1317-81-7-1659
/content/journal/jgv/10.1099/0022-1317-81-7-1659
/content/journal/jgv/10.1099/0022-1317-81-7-1659
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