@article{mbs:/content/journal/jgv/10.1099/0022-1317-81-5-1347, author = "Liang, Guo-Dong and Li, Lei and Zhou, Guo-Lin and Fu, Shi-Hong and Li, Qi-Ping and Li, Fu-Sheng and He, Hai-Huai and Jin, Qi and He, Ying and Chen, Bo-Quan and Hou, Yun-De", title = "Isolation and complete nucleotide sequence of a Chinese Sindbis-like virus", journal= "Journal of General Virology", year = "2000", volume = "81", number = "5", pages = "1347-1351", doi = "https://doi.org/10.1099/0022-1317-81-5-1347", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-5-1347", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Infection with alphaviruses is common in the Chinese population. Here we report the isolation of a Sindbis-like virus from a pool of Anopheles mosquitoes collected in Xinjiang, China during an arbovirus survey. This virus, designated XJ-160, rapidly produced cytopathic effects on mosquito and hamster cells. In addition, it was lethal to neonatal mice if inoculated intracerebrally. Serologically, XJ-160 reacted with and was neutralized by an anti-Sindbis antibody. Anti-XJ-160 antibodies were found in several cohorts of Chinese subjects. The complete 11626-base nucleotide sequence of XJ-160 was determined. XJ-160 has diverged significantly from the prototype Sindbis virus, with an 18% difference in nucleotide sequence and an 8ยท6% difference in amino acids; there are 11 deletions and 2 insertions, involving 99 nucleotides in total. XJ-160 is most closely linked to Kyzylagach virus isolated in Azerbaijan. Both belong to the African/European genetic lineage of Sindbis virus, albeit more distantly related to other members.", }