1887

Abstract

Plasmids that expressed the nucleocapsid, haemagglutinin and fusion proteins of measles virus (MV) were used to immunize cotton rats () against intranasal MV infection. After immunization with all three plasmids, T cell responses and MV-specific antibodies were induced. A reduction in virus titre was observed in lung tissue from animals immunized with plasmids expressing the viral glycoproteins. Histologically, however, a moderate peribronchitis was observed after immunization with the plasmid expressing the fusion protein whereas, after immunization with plasmids expressing haemagglutinin or both glycoproteins, only mild or focal peribronchitis was seen. Immunization with the nucleocapsid did not reduce virus titres, probably because of the failure to induce neutralizing antibodies. A disadvantage of plasmid immunization was its inefficacy in the presence of MV-specific ‘maternal’ antibodies. This indicates that genetic immunization has to be improved to be a useful alternative vaccine against measles.

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2000-05-01
2019-10-20
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References

  1. Bankamp, B., Brinckmann, U. G., Reich, A., Niewiesk, S., ter Meulen, V. & Liebert, U. G. ( 1991; ). Measles virus nucleocapsid protein protects rats from encephalitis. Journal of Virology 65, 1695-1700.
    [Google Scholar]
  2. Boyle, J. S., Silva, A., Brady, J. L. & Lew, A. M. ( 1997; ). DNA immunization: induction of higher avidity antibody and effect of route on T cell cytotoxicity. Proceedings of the National Academy of Sciences, USA 94, 14626-14631.[CrossRef]
    [Google Scholar]
  3. Bruton, O. C. ( 1953; ). Agammaglobulinemia. Pediatrics 9, 722-728.
    [Google Scholar]
  4. Cardoso, A. I., Sixt, N., Vallier, A., Fayolle, J., Buckland, R. & Wild, T. F. ( 1998; ). Measles virus DNA vaccination: antibody isotype is determined by the method of immunization and by the nature of both the antigen and the coimmunized antigen. Journal of Virology 72, 2516-2518.
    [Google Scholar]
  5. Fennelly, G. J., Flynn, J. L., ter Meulen, V., Liebert, U. G. & Bloom, B. R. ( 1995; ). Recombinant bacille Calmette–Guérin priming against measles. Journal of Infectious Diseases 172, 698-705.[CrossRef]
    [Google Scholar]
  6. Fennelly, G. J., Khan, S. A., Abadi, M. A., Wild, T. F. & Bloom, B. R. ( 1999; ). Mucosal DNA vaccine immunization against measles with a highly attenuated Shigella flexneri vector. Journal of Immunology 162, 1603-1610.
    [Google Scholar]
  7. Finke, D. & Liebert, U. G. ( 1994; ). CD4+ T cells are essential in overcoming experimental murine measles encephalitis. Immunology 83, 184-189.
    [Google Scholar]
  8. Fooks, A. R., Schadeck, E., Liebert, U. G., Dowsett, A. B., Rima, B. K., Steward, M., Stephenson, J. R. & Wilkinson, G. W. G. ( 1995; ). High-level expression of the measles virus nucleocapsid protein by using a replication-deficient adenovirus vector: induction of an MHC-1-restricted CTL response and protection in a murine model. Virology 210, 456-465.[CrossRef]
    [Google Scholar]
  9. Fooks, A. R., Jeevarajah, D., Warnes, A., Wilkinson, G. W. G. & Clegg, J. C. S. ( 1996; ). Immunization of mice with plasmid DNA expressing the measles virus nucleoprotein gene. Viral Immunology 9, 65-71.[CrossRef]
    [Google Scholar]
  10. Good, R. A. & Zak, S. J. ( 1956; ). Disturbances in gamma globulin synthesis as ‘experiments of nature’. Pediatrics 18, 109-149.
    [Google Scholar]
  11. Griffin, D. E. ( 1995; ). Immune responses during measles virus infection. In Measles Virus, pp. 117-134. Edited by M. Billeter & V. ter Meulen. Berlin: Springer.
  12. Hassett, D. E., Zhang, J. & Whitton, J. L. ( 1997; ). Neonatal DNA immunization with a plasmid encoding an internal viral protein is effective in the presence of maternal antibodies and protects against subsequent viral challenge. Journal of Virology 71, 7881-7888.
    [Google Scholar]
  13. Huber, M., Cattaneo, R., Spielhofer, P., Örvell, C., Norrby, E., Messerli, M., Perriard, J. C. & Billeter, M. A. ( 1991; ). Measles virus phosphoprotein retains the nucleocapsid protein in the cytoplasm. Virology 185, 299-308.[CrossRef]
    [Google Scholar]
  14. Katz, M. ( 1995; ). Clinical spectrum of measles. In Measles Virus, pp. 1-12. Edited by M. Billeter & V. ter Meulen. Berlin: Springer.
  15. Koprowski, H. & Weiner, D. B. (editors) (1998). DNA Vaccination/Genetic Vaccination. Current Topics in Microbiology and Immunology Series, vol. 226. Berlin: Springer.
  16. Krieg, A. M., Yi, A. K., Schorr, J. & Davis, H. L. ( 1998; ). The role of CpG dinucleotides in DNA vaccines. Trends in Microbiology 6, 23-27.[CrossRef]
    [Google Scholar]
  17. Le Potier, M.-F., Monteil, M., Houdayer, C. & Eloit, M. ( 1997; ). Study of the delivery of the gD gene of pseudorabies virus to one-day-old piglets by adenovirus or plasmid DNA as ways to by-pass the inhibition of immune responses by colostral antibodies. Veterinary Microbiology 55, 75-80.[CrossRef]
    [Google Scholar]
  18. Martinez, X., Brandt, C., Saddallah, F., Tougne, C., Barrios, C., Wild, F., Dougan, G., Lambert, P. H. & Siegrist, C. A. ( 1997; ). DNA immunization circumvents deficient induction of T helper type 1 and cytotoxic T lymphocyte responses in neonates and during early life. Proceedings of the National Academy of Sciences, USA 94, 8726-8731.[CrossRef]
    [Google Scholar]
  19. Monteil, M., Le Potier, M.-F., Guillotin, J., Cariolet, R., Houdayer, C. & Eloit, M. ( 1996; ). Genetic immunization of seronegative one-day-old piglets against pseudorabies induces neutralizing antibodies but not protection and is ineffective in piglets from immune dams. Veterinary Research 27, 443-452.
    [Google Scholar]
  20. Nahmias, A. J., Griffith, D., Salsbury, C. & Yoshida, K. ( 1967; ). Thymic aplasia with lymphopenia, plasma cells, and normal immunoglobulins. Relation to measles virus infection. JAMA 201, 729-734.[CrossRef]
    [Google Scholar]
  21. Niewiesk, S., Eisenhuth, I., Fooks, A., Clegg, J. C. S., Schnorr, J.-J., Schneider-Schaulies, S. & ter Meulen, V. ( 1997; ). Measles virus-induced immune suppression in the cotton rat (Sigmodon hispidus) model depends on viral glycoproteins. Journal of Virology 71, 7214-7219.
    [Google Scholar]
  22. Ohishi, K., Inui, K., Yamanouchi, K. & Barrett, T. ( 1999; ). Cell-mediated immune responses in cattle vaccinated with a vaccinia virus recombinant expressing the nucleocapsid protein of rinderpest virus. Journal of General Virology 80, 1627-1634.
    [Google Scholar]
  23. Schlender, J., Schnorr, J.-J., Spielhofer, P., Cathomen, T., Cattaneo, R., Billeter, M. A., ter Meulen, V. & Schneider-Schaulies, S. ( 1996; ). Interaction of measles virus glycoproteins with the surface of uninfected peripheral blood lymphocytes induces immunosuppression in vitro. Proceedings of the National Academy of Sciences, USA 93, 13194-13199.[CrossRef]
    [Google Scholar]
  24. van Binnendijk, R. S., Poelen, M. C. M., van Amerongen, G., de Vries, P. & Osterhaus, A. D. M. E. ( 1997; ). Protective immunity in macaques vaccinated with live attenuated, recombinant, and subunit measles vaccines in the presence of passively acquired antibodies. Journal of Infectious Diseases 175, 524-532.[CrossRef]
    [Google Scholar]
  25. Wang, Y., Xiang, Z., Pasquini, S. & Ertl, H. C. J. ( 1998; ). Effect of passive immunization or maternally transferred immunity on the antibody response to a genetic vaccine to rabies virus. Journal of Virology 72, 1790-1796.
    [Google Scholar]
  26. Wild, T. F. ( 1999; ). Measles vaccines, new developments and immunization strategies. Vaccine 17, 1726-1729.[CrossRef]
    [Google Scholar]
  27. Wyde, P. R., Ambrose, M. W., Voss, T. G., Meyer, H. L. & Gilbert, B. E. ( 1992; ). Measles virus replication in lungs of hispid cotton rats after intranasal inoculation. Proceedings of the Society for Experimental Biology and Medicine 201, 80-87.[CrossRef]
    [Google Scholar]
  28. Wyde, P. R., Moore-Poveda, D. K., Daley, N. J. & Oshitani, H. ( 1999; ). Replication of clinical measles virus strains in hispid cotton rats. Proceedings of the Society for Experimental Biology and Medicine 221, 53-62.[CrossRef]
    [Google Scholar]
  29. Yamanouchi, K., Inui, K., Sugimoto, M., Asano, K., Nishimaki, F., Kitching, R. P., Takamatsu, H. & Barrett, T. ( 1993; ). Immunisation of cattle with a recombinant vaccinia vector expressing the haemagglutinin gene of rinderpest virus. Veterinary Record 132, 152-156.[CrossRef]
    [Google Scholar]
  30. Yang, K., Mustafa, F., Valsamakis, A., Santoro, J. C., Griffin, D. E. & Robinson, H. L. ( 1997; ). Early studies on DNA-based immunizations for measles virus. Vaccine 15, 888-891.[CrossRef]
    [Google Scholar]
  31. Zhu, Y. D., Fennelly, G., Miller, C., Tarara, R., Saxe, I., Bloom, B. & McChesney, M. ( 1997; ). Recombinant bacille Calmette–Guérin expressing the measles virus nucleoprotein protects infant rhesus macaques from measles virus pneumonia. Journal of Infectious Diseases 176, 1445-1453.[CrossRef]
    [Google Scholar]
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