RT Journal Article SR Electronic(1) A1 Nanan, Ralph A1 Rauch, Andrea A1 Kämpgen, Eckhart A1 Niewiesk, Stefan A1 Kreth, Hans Wolfgang YR 2000 T1 A novel sensitive approach for frequency analysis of measles virus-specific memory T-lymphocytes in healthy adults with a childhood history of natural measles JF Journal of General Virology, VO 81 IS 5 SP 1313 OP 1319 DO https://doi.org/10.1099/0022-1317-81-5-1313 PB Microbiology Society, SN 1465-2099, AB Measles virus (MV), a single-stranded negative-sense RNA virus, is an important pathogen causing almost 1 million deaths annually. Acute MV infection induces immunity against disease throughout life. The immunological factors which are responsible for protection against measles are still poorly understood. However, T-cell-mediated immune responses seem to play a central role. The emergence of new single-cell methods for quantification of antigen-specific T-cells directly ex vivo has prompted us to measure frequencies of MV-specific memory T-cells. As an indicator for T-cell activation IFN-γ production was measured. PBMC were analysed by intracellular staining and ELISPOT assay after stimulation with MV-infected autologous B-lymphoblastoid cell lines or dendritic cells. T-cell responses were exclusively seen with PBMC from MV-seropositive healthy adults with a history of natural measles in childhood. The median frequency of MV-specific T-cells was 0·35% for CD3+CD4+ and 0·24% for the CD3+CD8+ T-cell subset. These frequencies are comparable with T-cell numbers reported by other investigators for persistent virus infections such as Epstein–Barr virus, cytomegalovirus or human immunodeficiency virus. Hence, this study illustrates that MV-specific CD4+ and CD8+ T-cells are readily detectable long after the acute infection, and thus are probably contributing to long-term immunity. Furthermore, this new approach allows efficient analysis of T-cell responses from small samples of blood and could therefore be a useful tool to further elucidate the role of cell-mediated immunity in measles as well as in other viral infections., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-5-1313