1887

Abstract

We report the molecular characterization, with subtyping of both K1 and K14.1/K15 genomic regions, of seven new human herpesvirus-8 (HHV-8) strains from Russian patients with classical Kaposi’s sarcoma. Phylogenetic studies, based on the complete K1 gene/protein analysis, indicate that six of these strains belong to the A subtype, with one belonging to the A4 group and exhibiting a unique deletion of 19 amino acids in the VR2 region at position 186–204. PCR-based studies of the K14.1/K15 genomic region indicate that four of the new strains were of the M subtype while three belonged to the P subtype. Our study indicates an important genetic diversity of the HHV-8 strains currently present in Russia, including a new peculiar strain possessing a unique deletion in the VR2 segment, and confirms the absence of correlation between the K1 and K14.1/K15 molecular subtypes, as M and P genotypes can be observed in the A K1 subtype.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-5-1217
2000-05-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/5/0811217a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-5-1217&mimeType=html&fmt=ahah

References

  1. Boshoff, C. & Weiss, R. A. ( 1998; ). Kaposi’s sarcoma-associated herpesvirus. Advances in Cancer Research 75, 57-86.
    [Google Scholar]
  2. Chang, Y., Cesarman, E., Pessin, M. S., Lee, F., Culpepper, J., Knowles, D. M. & Moore, P. S. ( 1994; ). Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi’s sarcoma. Science 266, 1865-1869.[CrossRef]
    [Google Scholar]
  3. Cook, P. M., Whitby, D., Calabro, M. L., Luppi, M., Kakoola, D. N., Hjalgrim, H., Ariyoshi, K., Ensoli, B., Davison, A. J. & Schulz, T. F. ( 1999; ). Variability and evolution of Kaposi’s sarcoma-associated herpesvirus in Europe and Africa. International Collaborative Group. AIDS 13, 1165-1176.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. (1993). PHYLIP version 3.5c, University of Washington, Seattle, USA.
  5. Fouchard, N., Lacoste, V., Couppie, P., Develoux, M., Mauclère, P., Michel, P., Herve, V., Pradinaud, R., Bestetti, G., Huerre, M., Tekaia, F., De Thé, G. & Gessain, A. ( 2000; ). Detection and genetic polymorphism of human herpesvirus type 8 in endemic or epidemic Kaposi’s sarcoma from west and central Africa, and south America. International Journal of Cancer 85, 166-170.[CrossRef]
    [Google Scholar]
  6. Kasolo, F. C., Monze, M., Obel, N., Anderson, R. A., French, C. & Gompels, U. A. ( 1998; ). Sequence analyses of human herpesvirus-8 strains from both African human immunodeficiency virus-negative and -positive childhood endemic Kaposi’s sarcoma show a close relationship with strains identified in febrile children and high variation in the K1 glycoprotein. Journal of General Virology 79, 3055-3065.
    [Google Scholar]
  7. Meng, Y. X., Spira, T. J., Bhat, G. J., Birch, C. J., Druce, J. D., Edlin, B. R., Edwards, R., Gunthel, C., Newton, R., Stamey, F. R., Wood, C. & Pellett, P. E. ( 1999; ). Individuals from North America, Australasia, and Africa are infected with four different genotypes of human herpesvirus 8. Virology 261, 106-119.[CrossRef]
    [Google Scholar]
  8. Poole, L. J., Zong, J. C., Ciufo, D. M., Alcendor, D. J., Cannon, J. S., Ambinder, R., Orenstein, J. M., Reitz, M. S. & Hayward, G. S. ( 1999; ). Comparison of genetic variability at multiple loci across the genomes of the major subtypes of Kaposi’s sarcoma-associated herpesvirus reveals evidence for recombination and for two distinct types of open reading frame K15 alleles at the right-hand end. Journal of Virology 73, 6646-6660.
    [Google Scholar]
  9. Russo, J. J., Bohenzky, R. A., Chien, M. C., Chen, J., Yan, M., Maddalena, D., Parry, J. P., Peruzzi, D., Edelman, I. S., Chang, Y. & Moore, P. S. ( 1996; ). Nucleotide sequence of the Kaposi sarcoma-associated herpesvirus (HHV8). Proceedings of the National Academy of Sciences, USA 93, 14862-14867.[CrossRef]
    [Google Scholar]
  10. Schulz, T. F. ( 1998; ). Kaposi’s sarcoma-associated herpesvirus (human herpesvirus-8). Journal of General Virology 79, 1573-1591.
    [Google Scholar]
  11. Zong, J. C., Ciufo, D. M., Alcendor, D. J., Wan, X., Nicholas, J., Browning, P. J., Rady, P. L., Tyring, S. K., Orenstein, J. M., Rabkin, C. S., Su, I. J., Powell, K. F., Croxson, M., Foreman, K. E., Nickoloff, B. J., Alkan, S. & Hayward, G. S. ( 1999; ). High-level variability in the ORF-K1 membrane protein gene at the left end of the Kaposi’s sarcoma-associated herpesvirus genome defines four major virus subtypes and multiple variants or clades in different human populations. Journal of Virology 73, 4156-4170.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-5-1217
Loading
/content/journal/jgv/10.1099/0022-1317-81-5-1217
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error