1887

Abstract

nucleopolyhedrovirus (NPV) is the major pathogen of the New Zealand endemic pasture pest, spp. To characterize this potential biological control agent, the genome of a virus isolated from was purified and cloned. The complete genome was cloned as HI or dIII restriction fragments, which were mapped by Southern hybridization and restriction analysis. To verify the physical map, the junctions between all dIII fragments were confirmed by sequencing. The viral genome was estimated to be 128 kbp. Sequence data generated at the termini of cloned restriction fragments were compared to sequence databases to identify putative gene homologues. Seventeen putative ORFs, which were homologous to other baculoviral sequences, were identified. These putative ORFs were located on the NPV physical map and their distribution was compared to genetic maps of NPVs isolated from , and . Although the virus from was significantly different from these other NPVs, a core region of the viral genome was conserved.

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2000-04-01
2019-10-23
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