When expressed in transgenic tobacco plants, transgene mRNA that includes the 3′ untranslated region (3′ UTR) of served as template for synthesis of complementary (−)-strand RNA following an infection by , or , but not when infected with . Deletion of the 3′ UTR from the transgene abolished the synthesis of (−)-strand transcripts. Similar results were obtained in transgenic tobacco plants expressing mRNA that includes the RNA3 3′ UTR of when infected with . These results show that the viral RNA-dependent RNA polymerase of several potyviruses and have the ability to recognize heterologous 3′ UTRs when included in transgene mRNAs, and to use them as transcription promoters.


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