%0 Journal Article %A Tunbäck, Petra %A Liljeqvist, Jan-Ake %A Löwhagen, Gun-Britt %A Bergström, Tomas %T Glycoprotein G of herpes simplex virus type 1: identification of type-specific epitopes by human antibodies %D 2000 %J Journal of General Virology, %V 81 %N 4 %P 1033-1040 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-81-4-1033 %I Microbiology Society, %X Serological diagnosis of herpes simplex virus (HSV) infections requires assays based on antigens that expose type-specific determinants. This study was designed to outline the B-cell epitopes of the type-specific glycoprotein G-1 (gG-1) of HSV type 1 (HSV-1), by investigating the reactivity of human anti-gG-1 antibodies, purified from 21 HSV-1-isolation-proven patient sera, to cellulose-bound synthetic peptides spanning the entire gG-1 sequence. The epitope mapping demonstrated that these antibodies bound preferentially to antigenic determinants that localized to regions with a high degree of amino acid similarity to the corresponding glycoprotein in HSV-2, gG-2. In spite of this, the purified anti-gG-1 antibodies were found to be non-reactive to native gG-2 antigen, as well as to overlapping gG-2 peptides, thus supporting the role of gG-1 as a prototype HSV-1 type-specific antigen. One immunodominant region, delimited by amino acids 112–127, reacted with all purified anti-gG-1 antibodies and may be of interest for the further development of a peptide-based HSV-1 type-specific seroassay. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-4-1033