1887

Abstract

Serological diagnosis of herpes simplex virus (HSV) infections requires assays based on antigens that expose type-specific determinants. This study was designed to outline the B-cell epitopes of the type-specific glycoprotein G-1 (gG-1) of HSV type 1 (HSV-1), by investigating the reactivity of human anti-gG-1 antibodies, purified from 21 HSV-1-isolation-proven patient sera, to cellulose-bound synthetic peptides spanning the entire gG-1 sequence. The epitope mapping demonstrated that these antibodies bound preferentially to antigenic determinants that localized to regions with a high degree of amino acid similarity to the corresponding glycoprotein in HSV-2, gG-2. In spite of this, the purified anti-gG-1 antibodies were found to be non-reactive to native gG-2 antigen, as well as to overlapping gG-2 peptides, thus supporting the role of gG-1 as a prototype HSV-1 type-specific antigen. One immunodominant region, delimited by amino acids 112–127, reacted with all purified anti-gG-1 antibodies and may be of interest for the further development of a peptide-based HSV-1 type-specific seroassay.

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2000-04-01
2019-12-05
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References

  1. Ackermann, G., Ackermann, F., Eggers, H. J., Wieland, U. & Kühn, J. E. ( 1998; ). Mapping of linear antigenic determinants on glycoprotein C of herpes simplex virus type 1 and type 2 recognized by human serum immunoglobulin G antibodies. Journal of Medical Virology 55, 281-287.[CrossRef]
    [Google Scholar]
  2. Ashley, R. L. ( 1993; ). Laboratory techniques in the diagnosis of herpes simplex virus infection. Genitourinary Medicine 69, 174-183.
    [Google Scholar]
  3. Ashley, R., Cent, A., Maggs, V., Nahmias, A. & Corey, L. ( 1991; ). Inability of enzyme immunoassays to discriminate between infections with herpes simplex virus type 1 and 2. Annals of Internal Medicine 115, 520-526.[CrossRef]
    [Google Scholar]
  4. Ashley, R. L., Wu, L., Pickering, J. W., Tu, M.-C. & Schnorenberg, L. ( 1998; ). Premarket evaluation of a commercial glycoprotein G-based enzyme immunoassay for herpes simplex virus type-specific antibodies. Journal of Clinical Microbiology 36, 294-295.
    [Google Scholar]
  5. Bergström, T. & Trybala, E. ( 1996; ). Antigenic differences between HSV-1 and HSV-2 glycoproteins and their importance for type-specific serology. Intervirology 39, 176-184.
    [Google Scholar]
  6. Bergström, T., Sjögren-Jansson, E., Jeansson, S. & Lycke, E. ( 1992; ). Mapping neuroinvasiveness of the herpes simplex virus type 1 encephalitis-inducing strain 2762 by the use of monoclonal antibodies. Molecular and Cellular Probes 6, 41-49.[CrossRef]
    [Google Scholar]
  7. Corey, L. ( 1994; ). The current trend in genital herpes. Progress in prevention. Sexually Transmitted Diseases 21, S38-S44.
    [Google Scholar]
  8. Corey, L. ( 1998; ). Raising the consciousness for identifying and controlling viral STDs: fears and frustrations. Sexually Transmitted Diseases 25, 58-69.[CrossRef]
    [Google Scholar]
  9. Dolter, K. E., Goins, W. F., Levine, M. & Glorioso, J. C. ( 1992; ). Genetic analysis of type-specific antigenic determinants of herpes simplex virus glycoprotein C. Journal of Virology 66, 4864-4873.
    [Google Scholar]
  10. Elion, G. B. ( 1982; ). Mechanism of action and selectivity of acyclovir. American Journal of Medicine 73, 7-13.[CrossRef]
    [Google Scholar]
  11. Field, P. R., Ho, D. W. T., Irving, W. L., Isaacs, D. & Cunningham, A. L. ( 1993; ). The reliability of serological tests for the diagnosis of genital herpes: a critique. Pathology 25, 175-179.[CrossRef]
    [Google Scholar]
  12. Forsgren, M., Skoog, E., Jeansson, S., Olofsson, S. & Giesecke, J. ( 1994; ). Prevalence of antibodies to herpes simplex virus in pregnant women in Stockholm in 1969, 1983 and 1989: implications for STD epidemiology. International Journal of STD & AIDS 5, 113-116.
    [Google Scholar]
  13. Frank, R. ( 1992; ). Spot-synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support. Tetrahedron 48, 9217-9232.[CrossRef]
    [Google Scholar]
  14. Gao, B. & Esnouf, M. P. ( 1996; ). Multiple interactive residues of recognition: elucidation of discontinuous epitopes with linear peptides. Journal of Immunology 157, 183-188.
    [Google Scholar]
  15. Grabowska, A., Jameson, C., Laing, P., Jeansson, S., Sjögren-Jansson, E., Taylor, J., Cunningham, A. & Irving, W. L. ( 1999; ). Identification of type-specific domains within glycoprotein G of herpes simplex virus type 2 (HSV-2) recognized by the majority of patients infected with HSV-2, but not by those infected with HSV-1. Journal of General Virology 80, 1789-1798.
    [Google Scholar]
  16. Gunalp, A. ( 1965; ). Growth and cytopathic effect of rubella virus in a line of green monkey kidney cells. Proceedings of the Society for Experimental Biology and Medicine 118, 85-90.[CrossRef]
    [Google Scholar]
  17. Ho, D. W. T., Field, P. R., Sjögren-Jansson, E., Jeansson, S. & Cunningham, A. L. ( 1992; ). Indirect ELISA for the detection of HSV-2 specific IgG and IgM antibodies with glycoprotein G (gG-2). Journal of Virological Methods 36, 249-264.[CrossRef]
    [Google Scholar]
  18. Jeansson, S., Forsgren, M. & Svennerholm, B. ( 1983; ). Evaluation of solubilized herpes simplex virus membrane antigen by enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 18, 1160-1166.
    [Google Scholar]
  19. Kramer, A., Schuster, A., Reineke, U., Malin, R., Volkmer-Engert, R., Landgraf, C. & Schneider-Mergener, J. ( 1994; ). Combinatorial cellulose-bound peptide libraries: screening tools for the identification of peptides that bind ligands with predefined specificity. Methods 6, 388-395.[CrossRef]
    [Google Scholar]
  20. Laver, W. G., Air, G. M., Webster, R. G. & Smith-Gill, S. J. ( 1990; ). Epitopes on protein antigens: misconceptions and realities. Cell 61, 553-556.[CrossRef]
    [Google Scholar]
  21. Lee, F. K., Coleman, R. M., Pereira, L., Bailey, P. D., Tatsuno, M. & Nahmias, A. J. ( 1985; ). Detection of herpes simplex virus type 2-specific antibody with glycoprotein G. Journal of Clinical Microbiology 22, 641-644.
    [Google Scholar]
  22. Lee, F. K., Pereira, L., Griffin, C., Reid, E. & Nahmias, A. ( 1986; ). A novel glycoprotein for detection of herpes simplex virus type 1-specific antibodies. Journal of Virological Methods 14, 111-118.[CrossRef]
    [Google Scholar]
  23. Leinikki, P., Lehtinen, M., Hyöty, H., Parkkonen, P., Kantanen, M. L. & Hakulinen, J. ( 1993; ). Synthetic peptides as diagnostic tools in virology. Advances in Virus Research 42, 149-186.
    [Google Scholar]
  24. Liljeqvist, J.-Å., Trybala, E., Svennerholm, B., Jeansson, S., Sjögren-Jansson, E. & Bergström, T. ( 1998; ). Localization of type-specific epitopes of herpes simplex virus type 2 glycoprotein G recognized by human and mouse antibodies. Journal of General Virology 79, 1215-1224.
    [Google Scholar]
  25. McGeoch, D. J., Dolan, A., Donald, S. & Rixon, F. J. ( 1985; ). Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. Journal of Molecular Biology 181, 1-13.[CrossRef]
    [Google Scholar]
  26. McGeoch, D. J., Moss, H. W. M., McNab, D. & Frame, M. C. ( 1987; ). DNA sequence and genetic content of the HindIII l region in the short unique component of the herpes simplex virus type 2 genome: identification of the gene encoding glycoprotein G, and evolutionary comparisons. Journal of General Virology 68, 19-38.[CrossRef]
    [Google Scholar]
  27. Marsden, H. S., MacAulay, K., Murray, J. & Smith, I. W. ( 1998; ). Identification of an immunodominant sequential epitope in glycoprotein G of herpes simplex virus type 2 that is useful for serotype-specific diagnosis. Journal of Medical Virology 56, 79-84.[CrossRef]
    [Google Scholar]
  28. Mertz, G. J. ( 1990; ). Genital herpes simplex virus infections. Medical Clinics of North America 74, 1433-1454.
    [Google Scholar]
  29. Nahmias, A. J., Lee, F. K. & Beckman-Nahmias, S. ( 1990; ). Sero-epidemiological and -sociological patterns of herpes simplex virus infection in the world. Scandinavian Journal of Infectious Diseases Supplementum 69, 19-36.
    [Google Scholar]
  30. Nilheden, E., Jeansson, S. & Vahlne, A. ( 1983; ). Typing of herpes simplex virus by an enzyme-linked immunosorbent assay with monoclonal antibodies. Journal of Clinical Microbiology 17, 677-680.
    [Google Scholar]
  31. Olofsson, S., Lundström, M., Marsden, H., Jeansson, S. & Vahlne, A. ( 1986; ). Characterization of a herpes simplex virus type 2-specified glycoprotein with affinity for N-acetylgalactosamine-specific lectins and its identification as g92K or gG. Journal of General Virology 67, 737-744.[CrossRef]
    [Google Scholar]
  32. Pereira, F. A. ( 1996; ). Herpes simplex: evolving concepts. Journal of the American Academy of Dermatology 35, 503-520.[CrossRef]
    [Google Scholar]
  33. Reineke, U., Sabat, R., Kramer, A., Stigler, R.-D., Seifert, M., Michel, T., Volk, H.-D. & Schneider-Mergener, J. ( 1996; ). Mapping protein–protein contact sites using cellulose-bound peptide scans. Molecular Diversity 1, 141-148.[CrossRef]
    [Google Scholar]
  34. Rekabdar, E., Tunbäck, P., Liljeqvist, J.-Å. & Bergström, T. ( 1999; ). Variability of the glycoprotein G gene in clinical isolates of herpes simplex virus type 1. Clinical and Diagnostic Laboratory Immunology 6, 826-831.
    [Google Scholar]
  35. Sanchez-Martinez, D., Schmid, D. S., Whittington, W., Brown, D., Reeves, W. C., Chatterjee, S., Whitley, R. J. & Pellett, P. E. ( 1991; ). Evaluation of a test based on baculovirus-expressed glycoprotein G for detection of herpes simplex virus type-specific antibodies. Journal of Infectious Diseases 164, 1196-1199.[CrossRef]
    [Google Scholar]
  36. Svennerholm, B., Olofsson, S., Jeansson, S., Vahlne, A. & Lycke, E. ( 1984; ). Herpes simplex virus type-selective enzyme-linked immunosorbent assay with Helix pomatia lectin-purified antigens. Journal of Clinical Microbiology 19, 235-239.
    [Google Scholar]
  37. Towbin, H., Staehelin, T. & Gordon, J. ( 1979; ). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76, 4350-4354.[CrossRef]
    [Google Scholar]
  38. Van Regenmortel, M. H. V. ( 1995; ). Transcending the structuralist paradigm in immunology – affinity and biological activity rather than purely structural considerations should guide the design of synthetic peptide epitopes. Biomedical Peptides, Proteins & Nucleic Acids 1, 109-116.
    [Google Scholar]
  39. Van Regenmortel, M. H. V. ( 1996; ). Mapping epitope structure and activity: from one-dimensional prediction to four-dimensional description of antigenic specificity. Methods 9, 465-472.[CrossRef]
    [Google Scholar]
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