RT Journal Article SR Electronic(1) A1 van der Ende, M. E. A1 Guillon, C. A1 Boers, P. H. M. A1 Gruters, R. A. A1 Racz, P. A1 Tenner-Racz, K. A1 Osterhaus, A. D. M. E. A1 Schutten, M.YR 2000 T1 Broadening of coreceptor usage by human immunodeficiency virus type 2 does not correlate with increased pathogenicity in an in vivo model JF Journal of General Virology, VO 81 IS 2 SP 507 OP 513 DO https://doi.org/10.1099/0022-1317-81-2-507 PB Microbiology Society, SN 1465-2099, AB The pathogenic properties of four primary human immunodeficiency virus type 2 (HIV-2) isolates and two primary HIV-2 biological clones were studied in an in vivo human-to-mouse chimeric model. The cell-associated viral load and the ability to reduce the severity of the induced graft-versus-host disease symptoms, the CD4/CD8 ratio and the level of repopulation of the mouse tissues by the graft, were determined. All HIV-2 strains, irrespective of their in vitro biological phenotype, replicated to high titres and significantly reduced graft-versus-host disease symptoms as well as the CD4/CD8 ratios. Reduction of graft repopulation caused by infection with the respective HIV-2 strains showed that the in vitro replication rate, syncytium-inducing capacity and ability to infect human macrophages did influence the in vivo pathogenic potential whereas broadening of coreceptor usage did not., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-2-507