@article{mbs:/content/journal/jgv/10.1099/0022-1317-81-2-507, author = "van der Ende, M. E. and Guillon, C. and Boers, P. H. M. and Gruters, R. A. and Racz, P. and Tenner-Racz, K. and Osterhaus, A. D. M. E. and Schutten, M.", title = "Broadening of coreceptor usage by human immunodeficiency virus type 2 does not correlate with increased pathogenicity in an in vivo model", journal= "Journal of General Virology", year = "2000", volume = "81", number = "2", pages = "507-513", doi = "https://doi.org/10.1099/0022-1317-81-2-507", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-2-507", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The pathogenic properties of four primary human immunodeficiency virus type 2 (HIV-2) isolates and two primary HIV-2 biological clones were studied in an in vivo human-to-mouse chimeric model. The cell-associated viral load and the ability to reduce the severity of the induced graft-versus-host disease symptoms, the CD4/CD8 ratio and the level of repopulation of the mouse tissues by the graft, were determined. All HIV-2 strains, irrespective of their in vitro biological phenotype, replicated to high titres and significantly reduced graft-versus-host disease symptoms as well as the CD4/CD8 ratios. Reduction of graft repopulation caused by infection with the respective HIV-2 strains showed that the in vitro replication rate, syncytium-inducing capacity and ability to infect human macrophages did influence the in vivo pathogenic potential whereas broadening of coreceptor usage did not.", }