1887

Abstract

The pathogenic properties of four primary human immunodeficiency virus type 2 (HIV-2) isolates and two primary HIV-2 biological clones were studied in an human-to-mouse chimeric model. The cell-associated viral load and the ability to reduce the severity of the induced graft-versus-host disease symptoms, the CD4/CD8 ratio and the level of repopulation of the mouse tissues by the graft, were determined. All HIV-2 strains, irrespective of their biological phenotype, replicated to high titres and significantly reduced graft-versus-host disease symptoms as well as the CD4/CD8 ratios. Reduction of graft repopulation caused by infection with the respective HIV-2 strains showed that the replication rate, syncytium-inducing capacity and ability to infect human macrophages did influence the pathogenic potential whereas broadening of coreceptor usage did not.

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2000-02-01
2019-10-19
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References

  1. Albert, J., Naucler, A., Bottiger, B., Broliden, P. A., Albino, P., Ouattara, S. A., Bjorkegren, C., Valentin, A., Biberfeld, G. & Fenyo, E. M. ( 1990; ). Replicative capacity of HIV-2, like HIV-1, correlates with severity of immunodeficiency. AIDS 4, 291–295.[CrossRef]
    [Google Scholar]
  2. Asjö, B., Albert, J., Karlsson, A., Morfeld-Manson, L., Biberfeld, G., Lidman, K. & Fenyo, E. M. (1986). Replicative properties of human immunodeficiency virus from patients with varying severity of HIV infection. Lancet ii, 660–662.
  3. Barre-Sinoussi, F., Chermann, J. C., Rey, R., Nugeyre, M. T., Chamaret, S., Gruest, J., Dauget, C., Rozenbaum, W. & Montagnier, L. ( 1983; ). Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome. Science 220, 868-871.[CrossRef]
    [Google Scholar]
  4. Berger, E. A., Doms, R. W., Fenyo, E. M., Korber, B. T., Littman, D. R., Moore, J. P., Sattentau, Q. J., Schuitemaker, H., Sodroski, J. & Weiss, R. A. ( 1998; ). A new classification for HIV-1. Nature 391, 240.[CrossRef]
    [Google Scholar]
  5. Cheng-Mayer, C., Seto, D., Tateno, M. & Levy, J. A. ( 1988; ). Biologic features of HIV-1 that correlate with virulence in the host. Science 240, 80-82.[CrossRef]
    [Google Scholar]
  6. Clavel, F., Guetard, D., Brun-Vezinet, F., Chamaret, S., Rey, M. A., Santos-Ferreira, M. O., Laurent, A., Dauget, C., Katlama, C., Rouzioux, C., Klatzmann, D., Champalimaud, J. L. & Montagnier, L. ( 1986; ). Isolation of a new retrovirus from West African patients with AIDS. Science 233, 343-346.[CrossRef]
    [Google Scholar]
  7. Dean, M., Carrington, M., Winkler, C., Huttley, G. A., Smith, M. W., Allikmets, R., Goedert, J. J., Buchbinder, S. P., Vittinghoff, E., Gomperts, E., Donfield, S., Vlahov, D., Kaslow, R., Saah, A., Rinaldo, C., Detels, R. & O’Brien, S. J. ( 1996; ). Genetic restriction of HIV-1 infection and progression to AIDS by a deletion allele of the CKR5 structural gene. Hemophilia Growth and Development Study, Multicenter AIDS Cohort Study, Multicenter Hemophilia Cohort Study, San Francisco City Cohort, ALIVE Study Science 273, 1856-1862.[CrossRef]
    [Google Scholar]
  8. Embretson, J., Zupanic, M., Ribas, J. L., Burke, A., Racz, P., Tenner-Racz, K. & Haase, A. T. ( 1993; ). Massive covert infection of helper T lymphocytes and macrophages by HIV during the incubation period of AIDS. Nature 362, 359-362.[CrossRef]
    [Google Scholar]
  9. Fenyo, E. M., Morfeld-Manson, L., Chiodi, F., Lind, B., Von Gegerfelt, A., Albert, J., Olausson, E. & Asjo, B. ( 1988; ). Distinct replicative and cytopathic characteristics of human immunodeficiency virus isolates. Journal of Virology 62, 4414-4419.
    [Google Scholar]
  10. Fox, C. H., Tenner-Racz, K., Racz, P., Firpo, A., Pizzo, P. A. & Fauci, A. S. ( 1991; ). Lymphoid germinal centers are reservoirs of human immunodeficiency virus type 1 RNA. Journal of Infectious Diseases 164, 1051-1057.[CrossRef]
    [Google Scholar]
  11. Groenink, M., Fouchier, R. A. M., De Goede, R. E. Y., De Wolf, F., Cuypers, H. T. M., Gruters, R. A., Huisman, H. G. & Tersmette, M. ( 1991; ). Phenotypical heterogeneity in a panel of infectious molecular HIV-1 clones derived from a single individual. Journal of Virology 65, 1968-1975.
    [Google Scholar]
  12. Gruters, R. A., Terpstra, F. G., De Jong, R., Van Noesel, C. J. M., Van Lier, R. A. W. & Miedema, F. ( 1990; ). Selective loss of T-cell functions in different stages of HIV infection. European Journal of Immunology 20, 1039-1044.[CrossRef]
    [Google Scholar]
  13. Gruters, R. A., Otto, S. A., Al, B. J. M., Verhoeven, A. J., Verweij, C. L., Van Lier, R. A. W. & Miedema, F. ( 1991; ). Non-mitogenic T-cell activation signals are sufficient for induction of HIV transcription. European Journal of Immunology 21, 167-172.[CrossRef]
    [Google Scholar]
  14. Guillon, C., van der Ende, M. E., Boers, P. H. M., Gruters, R. A., Schutten, M. & Osterhaus, A. D. M. E. ( 1998; ). Coreceptor usage of human immunodeficiency virus type 2 primary isolates and biological clones is broad and does not correlate with their syncytium-inducing capacities. Journal of Virology 72, 6260-6263.
    [Google Scholar]
  15. Huppes, W., De Geus, B., Zurcher, C. & van Bekkum, D. W. ( 1992; ). Acute human vs. mouse graft vs. host disease in normal and immunodeficient mice. European Journal of Immunology 22, 197-206.[CrossRef]
    [Google Scholar]
  16. Huppes, W., Paulonis, J., Dijk, H., Van Rooijen, N. & van Bekkum, D. W. ( 1993; ). The role of natural antibodies and ABO (H) blood groups in transplantation of human lymphoid cells into mice. European Journal of Immunology 23, 26-32.[CrossRef]
    [Google Scholar]
  17. Levy, J. A., Cheng-Mayer, C., Dina, D. & Luciw, P. A. ( 1986; ). Aids retrovirus (ARV-2) clone replicates in transfected human and animal fibroblasts. Science 232, 998-1001.[CrossRef]
    [Google Scholar]
  18. McKnight, A., Dittmar, M. T., Moniz-Periera, J., Ariyoshi, K., Reeves, J. D., Hibbitts, S., Whitby, D., Aarons, E., Proudfoot, A. E., Whittle, H. & Clapham, P. R. ( 1998; ). A broad range of chemokine receptors are used by primary isolates of human immunodeficiency virus type 2 as coreceptors with CD4. Journal of Virology 72, 4065-4071.
    [Google Scholar]
  19. Marlink, R., Kanki, P., Thior, I., Travers, K., Eisen, G., Siby, T., Traore, I., Hsieh, C. C., Dia, M. C. & Gueye, E. H. ( 1994; ). Reduced rate of disease development after HIV-2 infection as compared to HIV-1. Science 265, 1587-1590.[CrossRef]
    [Google Scholar]
  20. Meyaard, L., Schuitemaker, H. & Miedema, F. ( 1993; ). T-cell dysfunction in HIV infection: anergy due to defective antigen presenting cell function? Immunology Today 14, 161-164.[CrossRef]
    [Google Scholar]
  21. Norton, A. J., Jordan, S. & Yeomans, P. ( 1994; ). Brief, high-temperature heat denaturation (pressure cooking): a simple and effective method of antigen retrieval for routinely processed tissues. Journal of Pathology 173, 371-379.[CrossRef]
    [Google Scholar]
  22. Sabri, F., Chiodi, F. & Fenyo, E. M. ( 1996; ). Lack of correlation between V3 amino acid sequence and syncytium-inducing capacity of some HIV type 1 isolates. AIDS Research and Human Retroviruses 12, 855-858.[CrossRef]
    [Google Scholar]
  23. Samson, M., Libert, F., Doranz, B. J., Rucker, J., Liesnard, C., Farber, C. M., Saragosti, S., Lapoumeroulie, C., Cognaux, J., Forceille, C., Muyldermans, G., Verhofstede, C., Burtonboy, G., Georges, M., Imai, T., Rana, S., Yi, Y., Smyth, R. J., Collman, R. G., Doms, R. W., Vassart, G. & Parmentier, M. ( 1996; ). Resistance to HIV-1 infection in Caucasian individuals bearing mutant alleles of the CCR-5 chemokine receptor gene. Nature 382, 722-725.[CrossRef]
    [Google Scholar]
  24. Schuitemaker, H., Kootstra, N. A., De Goede, R. E. Y., De Wolf, F., Miedema, F. & Tersmette, M. ( 1991; ). Monocytotropic human immunodeficiency virus 1 (HIV-1) variants detectable in all stages of HIV infection lack T-cell line tropism and syncytium-inducing ability in primary T-cell culture. Journal of Virology 65, 356-363.
    [Google Scholar]
  25. Schuitemaker, H., Koot, M., Kootstra, N. A., Dercksen, M. W., De Goede, R. E. Y., Van Steenwijk, R. P., Lange, J. M. A., Eeftink Schattenkerk, J. K. M., Miedema, F. & Tersmette, M. ( 1992a; ). Biological phenotype of human immunodeficiency virus type 1 clones at different stages of infection: progression of disease is associated with a shift from monocytotropic to T-cell-tropic virus populations. Journal of Virology 66, 1354-1360.
    [Google Scholar]
  26. Schuitemaker, H., Kootstra, N. A., Groenink, M., De Goede, R. E. Y., Miedema, F. & Tersmette, M. ( 1992b; ). Differential tropism of clinical HIV-1 isolates for primary monocytes and promonocytic-cell lines. AIDS Research and Human Retroviruses 8, 1679-1682.[CrossRef]
    [Google Scholar]
  27. Schutten, M., Tenner-Racz, K., Racz, P., van Bekkum, D. W. & Osterhaus, A. D. M. E. ( 1996; ). Human antibodies that neutralize primary human immunodeficiency virus type 1 in vitro do not provide protection in an in vivo model. Journal of General Virology 77, 1667-1675.[CrossRef]
    [Google Scholar]
  28. Tenner-Racz, K., Stellbrink, H. J., van Lunzen, J., Schneider, C., Jacobs, J. P., Raschdorff, B., Grosschupff, G., Steinman, R. M. & Racz, P. ( 1998; ). The unenlarged lymph nodes of HIV-1-infected, asymptomatic patients with high CD4 T cell counts are sites for virus replication and CD4 T cell proliferation. The impact of highly active antiretroviral therapy. Journal of Experimental Medicine 187, 949-959.[CrossRef]
    [Google Scholar]
  29. Tersmette, M., Lange, J. M. A., De Goede, R. E. Y., De Wolf, F., Eeftink Schattenkerk, J. K. M., Schellekens, P. Th. A., Coutinho, R. A., Huisman, J. G., Goudsmit, J. & Miedema, F. (1989). Association between biological properties of human immunodeficiency virus variants and risk for AIDS and AIDS mortality Lanceti, 983–985.
  30. Tersmette, M. & Schuitemaker, H. ( 1993; ). Virulent HIV strains? AIDS 7, 1123-1125.[CrossRef]
    [Google Scholar]
  31. van Baalen, C. A., Pontesilli, O., Huisman, R. C., Geretti, A. M., Klein, M. R., De Wolf, F., Miedema, F., Gruters, R. A. & Osterhaus, A. D. M. E. ( 1997; ). Human immunodeficiency virus type 1 Rev- and Tat-specific cytotoxic T lymphocyte frequencies inversely correlate with rapid progression to AIDS Journal of General Virology 78, 1913-1918.
    [Google Scholar]
  32. van Baalen, C. A., Schutten, M., Huisman, R. C., Boers, P. H. M., Gruters, R. A. & Osterhaus, A. D. M. E. ( 1998; ). Kinetics of antiviral activity by human immunodeficiency virus type 1-specific cytotoxic T lymphocytes (CTL) and rapid selection of CTL escape virus in vitro Journal of Virology 72, 6851-6857.
    [Google Scholar]
  33. van der Ende, M. E., Schutten, M., Ly, T. D., Gruters, R. A. & Osterhaus, A. D. M. E. ( 1996; ). HIV-2 infection in 12 European residents: virus characteristics and disease progression AIDS 10, 1649-1655.[CrossRef]
    [Google Scholar]
  34. Whittle, H., Morris, J., Todd, J., Corrah, T., Sabally, S., Bangali, J., Ngom, P. T., Rolfe, M. & Wilkins, A. ( 1994; ). HIV-2-infected patients survive longer than HIV-1-infected patients AIDS 8, 1617-1620.[CrossRef]
    [Google Scholar]
  35. Zinkernagel, R. M. & Hengartner, H. ( 1994; ). T-cell-mediated immunopathology versus direct cytolysis by virus: implications for HIV and AIDS. Immunology Today 15, 262-268.[CrossRef]
    [Google Scholar]
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